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Showing 1 to 20 of 34 for “"aggrecan"”.
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Probing nanomechanics of aggrecan and the aggrecan-rich pericellular matrix of chondrocytes in cartilage
… of type II collagen and the proteoglycan, aggrecan, which in combination provide the tensile, shear, and compressive stiffness of the tissue. While the collagen network mainly provides resistance to tensile and shear deformation, aggrecan enmeshed within this network contributes …
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Aggrecan fragments as biomarkers in osteoarthritis
… on proteolytic degradation of the proteoglycan aggrecan with the overall aim to study the potential of aggrecan fragments as biomarkers in knee OA. Methodology/Principal findings: Using neoepitope specific antibodies in Western blots, aggrecan fragments were identified in knee cartilage and …
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Molecular studies of aggrecan : experiments and simulations
… disc, the large self-assembling proteoglycans, aggrecan, are essential for the disc in resisting multiaxial compressive loads. Aggrecan degradation, a combinational result of abnormal and/or reduced cellular synthesis and proteolytic cleavage, leads to a reduction in the disc functionality. The …
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Έκφραση και ρόλος της aggrecan και perlecan στο πρώιμο έμβρυο
… πρωτεογλυκάνες, εκ των οποίων οι perlecan και aggrecan είναι από τις πρώτες που εκφράζονται στο πρώιμο έμβρυο. Μελετήσαμε τη χωρο-χρονική κατανομή των perlecan και aggrecan με RT-PCR, ανοσοφθορισμό και ανοσοκατακρήμνιση από το στάδιο Χ (μορίδιο) έως το στάδιο ΗΗ17 (29-32 σωμίτες) στο πρώιμο …
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Ultrastructure and nanomechanical properties of aggrecan from native cartilage and engineered tissue
Electrostatic interactions associated with aggrecan, one of the major components of the cartilage extracellular matrix, are responsible for ~50% of the equilibrium compressive elastic modulus of the tissue. The bottle-brush-shaped aggrecan consists of a core protein to which ~100 sulfated …
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Nanomechanics of cartilage extracellular matrix macromolecules
… properties between opposing cartilage aggrecan macromolecules were probed. In addition, nanoscale dynamic oscillatory mechanical properties of cartilage and its type II collagen network was measured. Aggrecan shear nanomechanics was assessed via microcontact printing and lateral force …
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Nanomechanics of cartilage at the matrix and molecular levels
… less than the dimension of single collagen or aggrecan molecules. The magnitude lE*l and the phase angle p of the dynamic complex indentation modulus were first measured in the frequency range, f ~ 0.2-130 Hz. For fresh, normal cartilage, the experimental frequency and length-scale dependence …
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Developing osteoarthritis treatments through cartilage tissue engineering and molecular imaging
… force microscopy (AFM) can be used to image aggrecan, one of the main components of cartilage. Imaging studies were carried out using fetal bovine epiphyseal aggrecan to optimize previous extraction and sample preparation procedures, as well as an AFM imaging protocol, for samples containing …
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Cartilage mechanobiology and transcriptional effects of combined mechanical compression and IGF-1 stimulation on bovine cartilage explants
… gene expression levels to previously measured aggrecan biosynthesis levels, aggrecan synthesis is shown to be transcriptionally regulated by IGF- 1, whereas inhibition of aggrecan synthesis by compression is not transcriptionally regulated.
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Effects of sodium hyaluronate and triamcinolone acetonide on proteoglycan metabolism in equine articular chondrocytes treated with interleukin-1
… content, and mRNA levels of collagen type II, aggrecan, and COX-2. The high concentration of HA (2.0 mg/mL) increased GAG synthesis while the high concentration of TA (0.6 mg/mL) decreased loss of GAG into the media. Both the high concentration of HA and TA increased the total GAG content …
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In vitro model of injury/cytokine-induced cartilage catabolism modulated by dynamic compression, growth factors, and glucocorticoids
… joint injury. Strain-dependent modulation of aggrecan biosynthesis and degradation, aggrecanase cleavage of aggrecan, chondrocyte gene expression profiles and changes in cell viability (apoptosis) were observed. Results imply that appropriate biomechanical stimuli can be beneficial during …
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Forensic taphonomy: investigating the post mortem biochemical properties of cartilage and fungal succession as potential forensic tools
… cell vitality assays. Results demonstrate that aggrecan, a major structural proteoglycan, produces high (230kDa) and low (38kDa) molecular weight cross-reactive polypeptides (CRPs) within cartilage extracellular matrix. The 230kDa CRP degrades in a reproducible manner irrespective of the …
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CONNECTIVE TISSUE GROWTH FACTOR IS REQUIRED FOR CHONDROGENESIS
… markers (sox9, sox6, l-sox5, type II collagen, aggrecan, decorin and link protein). Real-Time PCR, Western blot and Immunofluorescence reveal that CCN2 is required for the early stages of chondrogenesis, including expression of sox6 and aggrecan, but not type II collagen. Furthermore, focal …
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Large Artery Stiffness: Genes and Pathways
… encoded proteins was also explored. Neither the aggrecan nor the fibulin-1 SNPs showed significant associations with ex vivo PWV in the donor aortas. The exonic aggrecan tagSNP rs2882676 displayed differential transcript abundance between homozygous allele carriers but this did not translate at …
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Self-assembling peptide hydrogels promote in vitro chondrogenesis of bone marrow-derived stromal cells : effects of peptide sequence, cell donor age, and method of growth factor delivery
… than young chondrocytes. Measurement of aggrecan core-protein and chondroitin-sulfate length by atomic-force microscopy revealed BMSCs produce longer core protein and chondroitin-sulfate, and fewer catabolic-cleavage products than chondrocytes. Therefore, BMSC-produced aggrecan appears to …
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Mechanobiology of the nucleus pulposus: The effect of hydrostatic pressure and glucose deprivation on nucleus pulposus cell sub‐populations
… brachyury. NC cells expressed significantly more aggrecan and GAG than MNP cells over the 7 day culture period. MNP cells responded to low pressure with an increase in total GAG while high pressure resulted in a significant decrease in aggrecan gene expression. NC cells showed no response in …
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Modeling and measurement of intermolecular interaction forces between cartilage ECM macromolecules
… that make up its extracellular matrix (ECM). Aggrecan is the most abundant proteoglycan in articular cartilage. It is composed of a core protein with highly charged, densely packed glycosaminoglycan (GAG) side chains, which are responsible for [approximately] 50% of the equilibrium compressive …
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Molekulare Zusammensetzung der extrazellulären Matrix im Patellar- und Quadrizepssehnenansatz frühkindlicher Patellae
… in den Anheftungszonen nachweisen, während Aggrecan in rund zwei drittel der Fälle in der kindlichen Enthesis auftritt. Histomorphologisch stellt sich die Enthesis an beiden Enden der Patella als Faserknorpel mit chondroiden, rundlichen Zellen dar, die in Reihe oder einzeln angeordnet sind. …
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Knee injuries and their consequences – the impact of impact
… metalloproteinase-induced cleavage of cartilage aggrecan. Moreover, findings from this model suggest that the aggrecan degradation may differ between cytokine-stimulated cartilage explants compared with cartilage explants mechanically injured and (or) co-incubated with joint capsule. …
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The human meniscus: transplantation, characterisation and tissue engineering
… cells with a CAM rich in type I collagen and aggrecan and lower quantities of type II collagen, while monolayer cultured meniscus cells have considerable amounts of type I and II collagen but almost no aggrecan in their CAM. Additional investigations led to the description of a new cell-type …
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