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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 226 for “"agarose"”.
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Manufacturing of agarose-based chromatographic adsorbents with controlled pore and particle size
… two processing parameters, the ionic strength of agarose solution and quenching temperature, on the structure of the resulting particles was investigated. Three characterization methods, Atomic Force and cryo-Scanning Electron microscopy, as well as mechanical testing of single particles where …
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The effect of dynamic shear force on chondrocyte biosynthesis in agarose gels
… explants as well as in hydrogels such as agarose and self-assembling peptides. Also, recent research has revealed that biosynthesis in cartilage explants can be increased by dynamic tissue shear loading as well. Thus, the purpose of this study was to investigate whether dynamic tissue …
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Evaluation of Prototype Cell Delivery Catheters Using Agarose Gel and Cell Culture Experiments
… bore and 2.0 mm large bore) were evaluated using agarose gel and cell culture experiments. We initially delivered pheochromocytoma (PC 12) cells through a 25-gauge syringe needle to optimize cell growth. We observed in the agarose gel experiments that when the inner catheter was filled and then …
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The Role of the Voltage Gradient in the Agarose Gel Electrophoresis of DNA
… equations for predicting DNA mobility during agarose gel electrophoresis (AGE) from voltage gradient are developed from the data of McDonnel (36) for electrophoresis in a 1.6% agarose gel. These equations represented the data well for DNA between 2 and 10 kilobase pairs (KBp) in length. A …
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Neural differentiation of pluripotent stem cells and applications to micropatterned agarose hydrogel for nerve guidance
… cells (mESCs), and (2) to employ micropatterned agarose hydrogels for nerve guidance. A protocol utilizing embryoid body (EB) formation, under both high and low glucose conditions, was devised for RA-induced neural lineage differentiation and RA/shh-induced oligodendrocyte differentiation. EBs …
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Surface-enhanced Raman scattering of metal nanoparticle assembly in agarose and highly ordered metal nanorod arrays
This thesis examines metal nanoparticle/agarose (MNPA) gel composites and highly ordered metal nanorod arrays, fabricated by guided nucleation during oblique angle deposition (OAD), as surface-enhanced Raman scattering (SERS) substrates. The effectiveness of MNPA has been effectively demonstrated …
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Chondrocytes in agarose culture : development of a mechanically funcional matrix, biosynthetic response to compression and molecular model of the modulus
Thesis (Ph. D.)--Massachusetts Institute of Technology, Whitaker College of Health Sciences and Technology, 1992.
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Hindered transport in composite hydrogels
… permeability through synthetic, two-fiber, agarose-dextran hydrogels at very small or very high Pe, respectively. By comparing diffusion and convection in the synthetic hydrogel with corresponding measurements in isolated rat GBM, further insight regarding the structure responsible for …
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Developing osteoarthritis treatments through cartilage tissue engineering and molecular imaging
… and assess the value of potential therapies. Agarose and self-assembling peptides have been used to make hydrogels for in vitro culture of bovine bone marrow stromal cells (BMSCs) which can differentiate into chondrocytes, undergo chondrogenesis, and produce cartilage tissue. So far, …
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Permeability studies in biomimetic glycosaminoglycan-hydrogel membranes
… interactions were studied using charged, fibrous agarose-glycosaminoglycan (GAG) membranes. The work was motivated by current research into the role of the glomerular capillary wall (GCW) in ultrafiltering blood plasma, which is the first step in the processing of blood by the kidney. The GCW is …
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Bacterial adhesion and detachment from model mineralized biofilms
… far, the research focus has been to create an agarose hydrogel with similar elastic moduli to real biofilms in order to perform systematic studies of calcium carbonate precipitation within the gel. Agarose is a polymer made of chains of alternating galactose and anhydrous galactose, whose …
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Elucidating the Role of Plakoglobin in Embryonic Stem Cell Culture
… stem cells were cultured in three-dimensional agarose microgels generated by droplet microfluidics. The three-dimensional matrix of the microgels supported the naïve pluripotency network and upregulated Plakoglobin. A cell-adhesion protein located in both adherens junctions and desmosomes. It …
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Micropatterned cell arrays for detecting DNA damage
… of the nuclear DNA in single cells embedded in agarose. In this assay, the size and shape of the extended 'comet tail' can be correlated to breaks in the DNA. This assay was first developed in the mid 1980s for non adherent cells such as lymphocytes; however, it has been plagued by technical …
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Isolation of a human milk sialyloligosaccharide by affinity chromatography with wheat germ agglutinin (WGA)
… labeled and applied to a highly substituted WGA-agarose column (20 mg/ml). Only a single component from the complete sialyloligosaccharide fraction was retarded in the WGA-agarose column. The WGA-bound fraction when applied to paper chromatography migrated with identical mobility as the …
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Tanned gelatin: some biophysical and biochemical properties of a new gel exclusion agent and its application to the chromatography of proteins and viruses
… their exclusion limits and rigidity. Agar and agarose have been found satisfactory for the separation of larger molecules including some viruses, but difficulties may arise at low gel concentrations due to the low mechanical strength of the granules. The dextran and polyacrylamide gels have in …
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Electrophoretic Mobilities of Topoisomers of ΦX174 DNA
… acid structure by zone electrophoresis in agarose gels has become an increasingly important method in studies requiring the separation and identification of species differing only slightly in molecular weight or conformation. We have demonstrated the existence of several circular DNA forms …
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Progression of chondrocyte signaling responses to mechanical stimulation in 3-D gel culture
… dynamic compression of chondrocytes in 3-D agarose culture. At early days in culture, blocking [alpha]v[beta]3 integrin abolished dynamic compression stimulation of proteoglycan synthesis, independent of effects in free-swell culture, while blocking [alpha]5[beta]1 integrins abolished the …
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Fabrication of Micropatterned Hydrogels for Cell Culture
… hydrogels and hybrid hydrogels by sources. Agarose, Collagen and Calcium alginate are the most popular natural hydrogels. Polyethylene (glycol) and its derivative Polyethylene (glycol) Diacrylate (PEGDA) are indispensable synthetic hydrogels. In this thesis, hydrogels are studied for their …
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INTERACTION OF CIBACRON BLUE 3G-A AND RELATED DYES WITH NUCLEOTIDE-REQUIRING ENZYMES
Cibacron Blue 3G-A substituted on an agarose or dextran matrix has become widely used as an absorbant for enzyme purification. Its mode of interaction with the proteins which bind to it is not entirely clear. One popular theory for proteins' attraction for the dye is that it is a specific …
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