Global ETD Search

Structure and function of PhyA from Desulfovibrio magneticus and use of its product in affinity pull-down experiments

Cleland, Colyn P., University of Lethbridge. Faculty of Arts and Science2019

lethbridge

Proteins and instability elements involved in phosphoenolpyruvate carboxykinase mRNA turnover

Hajarnis, Sachin Shrikant, author, Curthoys, Norman, advisor, Bamburg, James, committee member, Mykles, Donald, committee member, Laybourn, Paul, committee member, Wilusz, Jeffrey, committee memberColorado State University. Libraries2006

… RNA-gel shift assays, immunoblocking assays, affinity pull down assays, western blot technique and MALDI-TOF/TOF analyses. The identified proteins bind to AU- and CU-rich sequences and secondary structures found within the PEPCK 3'-UTR. A tetracycline regulated promoter system was utilized in …

colostate Repository record for Proteins and instability elements involved in phosphoenolpyruvate carboxykinase mRNA turnover (opens in a new tab)

Purification of protein phosphatases and 14-3-3-binding proteins and their regulation by the cAMP–PKA pathway

Reetoo, NamrataUniversity of Dundee2012

… activated and so started by doing a microcystin-affinity purification. Microcystin is a potent inhibitor of most PPP phosphatases and has been adapted to purify protein phosphatases from cellular extracts. I used the cAMP agonist forskolin to trigger activation of the cAMP–PKA pathway and used …

dundee Repository record for Purification of protein phosphatases and 14-3-3-binding proteins and their regulation by the cAMP–PKA pathway (opens in a new tab)