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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"affinity capture"”.
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Plasticity of the Nuclear Pore Complex Revealed With Proteomics
… I have combined multiple methodologies: affinity capture, metabolic labeling and mass spectrometry. Moreover, to identify the required affinity capture conditions I have developed a high-throughput screen, which has successfully worked with numerous protein complexes, in addition to the …
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Metal-protein interactions for proteomics applications
… the Tao lab introduced Polymer-based Metal Affinity Capture with Ti(IV) (PolyMAC-Ti) for phosphopeptide enrichment in solution phase as opposed to commonly used solid phase extraction methods. In chapter 2 we discuss further extension of PolyMAC technology to PolyMAC-Zr and the use of …
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Study and development of label-free optical biosensors for biomedical applications
… in microplate, microfluidic, and spot-based affinity capture assays. Chapter 3 shows the work on integration of PC biosensor with actuate-to-open valve microfluidic chip for pL-volume combinatorial mixing and screening application. In Chapter 4, the development and characterization of SERS …
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Redox Regulation of Type Iα PKA in the Heart
… not co-purified with WT or KI RIα after cAMP-affinity capture. LC-MS/MS showed that the PKA substrates, alpha-enolase and ChChd3 did co-purify, but the AKAPs associated with these (myomegalin and sphingosine kinase interacting protein, respectively) were not co-captured. Trx recycled disulfide …
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APPROACHES TO IDENTIFY SURFACE PROTEINS OF ANAPLASMA PHAGOCYTOPHILUM DENSE-CORED ORGANISMS AS ADHESINS TO HUMAN P-SELECTIN GLYCOPROTEIN LIGAND-1
… surface proteins as PSGL-1 adhesins, several affinity capture approaches were tested. We used commercially available recombinant human PSGL-1 (rhPSGL-1) to try and capture adhesins by crosslinking and affinity purification. We were unsuccessful, but nevertheless gained insight into the binding …
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Chemical and biological modification of porous silicon photonic crystals.
… the surface chemistry allows specific capture of live cells and incubation with stimulated macrophages in tissue culture results in optical detection of released gelatinase enzymes. The generality of the surface chemistry allows for a range of other biological applications to be …
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Development of Tyrosine Kinase Peptide Biosensors and Methods for Detection
… These modules included a biotin-tag for affinity capture, a photo-cleavable amino acid to allow release of the substrate from the delivery module and the cell penetrating peptides TAT. A live cell kinase assay utilizing this biosensor was developed to monitor intercellular Syk activity in …
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Design of Nuclear-Targeting Peptides for Macromolecule Delivery via Machine Learning
… and internalized peptides recovered via affinity capture. De novo sequencing was achieved by Orbitrap tandem mass spectrometry, and several unique, unnatural sequences were identified that could effectively deliver the antisense oligomer to the nucleus. In summary, machine learning and …
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DEVELOPMENT AND OPTIMIZATION OF ON-PROBE AFFINTY CAPTURE (OPAC) MALDI MASS SPECTROMETRY FOR THE FRACTIONATION AND ANALYSIS OF COMPLEX PROTEIN MIXTURES
… incorporated on the MALDI target. On-Probe Affinity Capture (OPAC) MALDI is a method that uses the RF pulsed plasma modified target surfaces for the protein purification, separation and identification all on the same single probe and one of the highest advantage of this method is the number …
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Identification of centrosomal proteomes using COMPACT, a novel tool for purification of centrosomes
… known as COMPACT (Centrosome Purification by Affinity Capture), involves a single-step affinity purification of centrosomes by a short peptide corresponding to a 33 amino acid C-terminal fragment of the centrosomal protein CCDC61. I have shown that the method is able to isolate centrosomes …
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Identification and Characterization of Novel Melioidosis Vaccine Candidates
… and then lysed to facilitate the affinity capture of biotinylated surface antigens. As expected, analysis of the antigens by SDS-PAGE indicated that only a subset of the B. pseudomallei proteome had been isolated, and that some of these antigens were differentially expressed. Using …
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MATRIX-ASSISTED LASER DESORPTION/IONIZATION (MALDI) TARGET MODIFICATION FOR ENHANCED PROTEOMICS ANALYSIS AND PLASMA POLYMER CHARACTERIZATION BY MALDI MASS SPECTROMETRY
… targets having reduced surface-protein binding affinity with a goal of increasing peptide/protein MALDI ion signals and lowering the limits of detection (LODs) for proteins and peptides. The second section discusses a bioselective MALDI target, produced via radio frequency (rf) plasma deposited …
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Microscale biosensors for HIV detection and viral load determination
… an antigen-based approach which leverages immuno-affinity recognition for whole virus particle detection. In this method, the novel component of our sensing system is an ion-filled liposome which, upon stimulation (in this case, by heating), releases ions into low-conductivity media in a …