Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 8 of 8 for “"aequorin"”.
-
Engineering, overexpression, purification and analytical applications of recombinant bioluminescent reporters.
… bacterial expression of the in vivo biotinylated aequorin photoprotein. The plasmid contained a biotin-acceptor coding sequence fused in-frame with the apoaequorin gene as well as the birA gene, encoding biotin protein ligase (BPL), responsible for post-translational biotinylation. The use of the …
-
An investigation into the role of cytosolic free Ca2+ in Salicylic acid mediation of disease resistance in Arabidopsis
… expressing the Ca2+-sensitive photoprotein aequorin, we previously reported a rapid and transient increase in [Ca2+]c upon application of exogenous SA. We now investigated the characteristics of the SA-induced [Ca2+]c increase and report that the majority of the response is derived from …
-
The involvement of ER calcium in abiotic stress tolerance
… imaging of Indo-1 and cytoplasmically-expressed aequorin luminescence, to detect transient changes in [Ca2+]cyt in response to a salt stimulus. There were no significant differences in [Ca2+]cyt measured by confocal ratio imaging between ER-CBP transgenic plants and control Arabidopsis plants in …
-
Einfluss der Antiarrhythmika Flecainid und Amiodaron auf die kontraktile Funktion und die Kraft-Frequenz-Beziehung des Herzens
… der intrazelluläre Kalziumstoffwechsel mit der Aequorin Methode untersucht. Ergebnisse: Der negativ inotrope Effekt von Flec ist direkt kalziumabhängig. Die KFB wird durch Flec deutlich verschlechtert. Flec hat am terminal insuffizienten und am nicht insuffizienten Myokard einen negativen Effekt …
-
The Role of Arabidopsis thaliana Cyclic Nucleotide-Gated Channels 2, 6, and 19 in Extracellular ATP Signalling
… channels in eATP signalling. By employing (apo)aequorin as a Ca2+ reporter, CNGC2 was found to be involved in Arabidopsis root’s [Ca2+]cyt responses to both eATP and eADP. Transcriptional analysis showed that the expression of CNGC6 and CNGC19 was upregulated by eATP in a DORN1/P2K1- and …
-
The Role of Arabidopsis thaliana Annexins 1, 2 and 4 in Extracellular ATP Signalling
… tested in $\it{A.}$ $\it{thaliana}$. Using (apo)aequorin, AtANN1 and ANNEXIN 2 (AtANN2) have been found to be involved in the root′s [Ca$^{2+}$]$_{cyt}$ response to both eATP and eADP whereas ANNEXIN 4 (AtANN4) might be acting as a negative regulator of AtANN1 (Chapters 3 and 4). Application of …
-
Study of the etiology of neurolathyrism, a human neurodegenerative disease with nutritional causes
… cells, using the luminescent protein aequorin (AEQ), specifically targeted towards one of these cellular compartments. A 24 h treatment of N2a neuroblastoma cells with sub-toxic concentrations of L-β- ODAP (but not L-glutamate) appeared to increase acute mitochondrial, endoplasmic and …
-
Phosphate starvation alters calcium signalling in roots of Arabidopsis thaliana
Low bioavailability of phosphate (P) due to low concentration and high immobility in soils is a key limiting factor in crop production. Application of excess amounts of P fertilizer is costly and by no means sustainable, as world-wide P resources are finite and running out. To facilitate the …