Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 38 for “"adhesion proteins"”.
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Investigating bacterial adhesion proteins as tools for controlling bacteria-surface interactions
Adhesins are cell-surface proteins that initiate target binding and surface colonization by bacteria on the path to infection and biofilm formation. A deeper understanding of the interactions between adhesins and their ligands may lead to the development of strategies for controlling infection and …
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STRUCTURAL AND FUNCTIONAL CHARACTERISATION OF MUCUS ADHESION PROTEINS OF LACTOBACILLUS REUTERI
… are thought to be key mediators in the mucus adhesion of commensal Lactobacillus species. However, knowledge on the structural or functional basis of adhesin interaction with mucin glycoproteins, the main component of mucus, is limited. This work describes the biochemical and structural …
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Characterization of a Novel Prostate Tumor-Associated Antigen
… showed that MAb TURP-27 and MAb HNK-1 bound glycoproteins of 180, 140, 120, 100, 90 and 69 kDa. Studies have shown that the HNK-1 carbohydrate epitope may be involved in cell adhesion and that it is a component of several characterized adhesion proteins. TURP-27 was found to bind at least three of …
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Investigation of Neural and Epithelial Cadherin Monomer Properties Using a Molecular Dynamics Approach
Neural and epithelial cadherin are adhesion proteins intensely studied by our lab. Although the sequence, structure, and conformation are similar for the two cadherins, there are several physical properties that differ: dimerization affinity, dimerization kinetics, intermediate structure, and …
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The Role of the YXXΦ Motif in CD82 Trafficking and Function
… suppressor. Tetraspanins are membrane spanning proteins that play critical roles in diverse biological and pathological processes, e.g., the regulation of cancer metastasis. CD82 is ubiquitously expressed in various types of tissues, but its expression becomes down-regulated or lost in a …
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Regulation of cadherin adhesion at intercellular junctions
Adhesion proteins maintain cell-cell interactions, which are critical for tissue formation and the hierarchical organization of all multicellular organisms, and among them, cadherins are the major transmembrane cell-cell adhesion proteins in all vertebrate tissues. Regulation of cadherin mediated …
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CD151 Reinforces Vascular Stability by Balancing Endothelial Cell Adhesion and Cytoskeletal Tension
… of endothelial cell-matrix and cell-cell adhesions, CD151 is needed for the optimal functions of various cell adhesion proteins such as integrin, cadherin, and CD44. The loss of CD151 elevates the cellular intrinsic contraction by upregulating RhoA signaling and downregulating of Rac1 …
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Analysis of the effects of BrdU on DLKP human lung cancer cells by two-dimensional difference gel electrophoresis and mass spectrometry
… in increased expression of the cytoskeletal proteins Keratin 8 and 18 and the cell adhesion proteins a2 and b1 integrin. This study investigated protein expression changes in differentiating DLKP cells following exposure to 10yM BrdU. DLKP cells were grown in culture flasks and harvested …
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Modulation of touch sensitivity in Caenorhabditis elegans
… signaling pathways, the integrin/focal adhesion signaling and insulin signaling, that modulate anterior touch sensitivity. Additional genes and pathways are also needed for optimal touch sensitivity in the TRNs, including the RAS/MAPK pathway, Rho-GTPases, cytoskeleton genes, and 43 …
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Towards a microfluidic disease detection deviced based on cellular adhesion differences
… developing microfluidic devices that exploit the adhesion differences between cell states and cell types to rapidly count cells of different types without the need for labels. There are two avenues in which to explore cell adhesion differences with these devices, the first is a net electrostatic …
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Design of multi-functional hydrogel for cell therapies
… stiffness, permeability, density of cell adhesion molecules, and mineralization capacity. The objective of this research is to decouple or tune the intricate dependencies between these hydrogel properties, in order to better understand and regulate cellular activities in a 3D matrix. The …
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A role for focal adhesion kinase in the contraction of gastric fundus smooth muscle evoked by cholinergic neurotransmission
… cytoskeleton to the extracellular matrix. Focal adhesion kinase (FAK) regulates the recruitment and assembly of focal adhesion proteins. It has been established that integrin proteins and FAK play important roles in cell adhesion, tension generation, and mechanotransduction. Integrins and FAK are …
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Investigating the mechanotransduction by two-photon fluorescence microscopy measurement of intracellular free energy of binding
… stress or relayed directly to the cell through adhesion complexes, is transmitted and translated into biological signals. This process is known as mechanotransduction. Extensive studies have been carried out on the signaling pathways involved in mechanotransduction. However, the mechanism(s) of …
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Cell culture platforms with cell-cell adhesive ligands for biomedical applications
… have been engineered to present several adhesion molecules that mimic cell-extracellular matrix interactions, many materials do not consider the effects of cell-cell interactions. One such molecule is N-cadherin, a cell-cell adhesion protein which is expressed on both MSCs and neurons. …
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Transcriptional Governance of Hair Follicle Stem Cell Quiescence and Niche Maintenance in Long-Term Tissue Regeneration
… proliferate. In turn, their expression of cell adhesion proteins remained low. As new hairs grew, wild-type (WT) HFSCs that had returned to quiescence and restored their repertoire of adhesion-associated proteins were able to anchor their bulge niche and the older hairs in place. However, FOXC1- …
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Cells, surfaces and adhesion
This thesis concerns cell adhesion to polymer surfaces with an experimental emphasis on hydrogels. The thesis begins with a review of the literature and a synthesis of recent evidence to describe the process of cell adhesion in a given situation. The importance of understanding integrin-adhesion …
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Proteomics based analysis of interactions between CEA cell adhesion molecule 1 (CEACAM1) and intracellular proteins in transfected tumor cells
CEA Cell Adhesion Molecule 1 (CEACAM1), a type 1 transmembrane protein belonging to the CEA gene family, is alternatively spliced to produce four major isoforms with a long (CEACAM1-L) or a short cytoplasmic domain (CEACAM1-S). Immunoprecipitations using the murine adenocarcinoma MC38 cell line …
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Integrative Genomics Reveals a Role for GNA13 in Lymphomagenesis
… alpha binding protein GNA13 and associated focal adhesion proteins. I aim to describe how loss-of-function mutations in GNA13 can be oncogenic in the context of germinal center B cell biology. Using in vitro techniques including liquid chromatography-mass spectrometry and knockdown and …
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Dynamics of Alpha5 Integrin, Paxillin, and Alpha-Actinin During Formation and Disassembly of Adhesions in Migratory Cells
… cell migration by fusing the genes encoding proteins involved in cell migration to the gene for a green fluorescent protein, GFP. I ascertained that the novel fusion protein expressed by cells transfected with alpha5-GFP is correctly localized, is surface-expressed in a heterodimeric form, …
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Patterned substrates for cell culture with electrohydrodynamic jet printing
… printing is introduced as a method to pattern adhesion proteins on hydrogel substrates for cell culture. First, a new technique to fabricate polyacrylamide substrates was developed and optimized. Hydrogels were formulated with acrylic acid and activated with N-hydroxysuccinimide. Protein …
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