Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 15 of 15 for “"adenylyltransferase"”.
-
Structure and Mechanism of a Eukaryotic FMN Adenylyltransferase
Flavin mononucleotide adenylyltransferase (FMNAT) catalyzes the formation of the essential flavocoenzyme FAD and plays an important role in flavocoenzyme homeostasis regulation. By sequence comparison, bacterial and eukaryotic FMNAT enzymes belong to two different protein superfamilies and …
-
Elaboration of 4′-Phosphopantetheine Adenylyltransferase inhibitors and Benzoxaboroles as antimalarial agents
… library of the bacterial 4′-Phosphopantetheine adenylyltransferase (PPAT) inhibitors obtained from AstraZeneca (AZ) containing two chemical series, viz. Ugi and cyclohexyl pyrimidine series, was subjected to the medium-throughput screening at the University of Cape Town (UCT)'s Holistic Drug …
-
THE EFFECTS OF NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE 2 (NMNAT2) ON MOUSE NERVE AND BLADDER DEVELOPMENT
Programmed cell death is one important component of neurodevelopment that, until recently, was thought to be a passive process based upon the lack of trophic support. There is now data that supports the compartmentalized neuron loss theory where each compartment of a nerve cell has a distinct …
-
A fragment-based approach towards understanding molecular recognition of 4’-phosphopantetheine adenylyltransferase from Mycobacterium abscessus
… approach targeting 4’-phosphopantetheine adenylyltransferase (PPAT) from Mycobacterium abscessus (Mabs). A fragment screen of the Abell laboratory 960-member library was conducted by Dr Sherine Thomas (Department of Biochemistry, University of Cambridge) and identified 15 hits validated by …
-
Biochemical and Cellular Characterization of NMN Adenylyltransferase 2: A Brain Specific Isoform of an Essential NAD Synthesizing Enzyme
NMN adenylyltransferase 2 (Nmnat2) is one of three vertebrate enzymes that catalyze the synthesis of NAD from NMN and ATP. Nmnat1, -2 and -3 are each expressed from a separate gene and are distinguished from one another by their expression, subcellular localization and enzyme kinetics. Nmnat …
-
Biochemical and Structures Studies of tRNA Modificaton and Repair Enzymes
… been shown to have kinase, phosphatase and adenylyltransferase activity. Therefore Pnkp/Hen1 might be involved in RNA repair. I cloned and expressed both proteins with a pETDuet vector. Pnkp and bHen1 form a heterotetramer in gel filtration column. Purfied Pnkp/bHEN1 efficiently repaired …
-
Biochemical and Structural Studies of RNA Modification and Repair
… has been shown to have kinase, phosphatase, and adenylyltransferase activities, which are hallmarks of RNA repair. However, Pnkp alone is not sufficient for ligation. Pnkp/Hen1 was shown to form a tetramer during purification in size exclusion gel filtration. I carried out biochemical assays to …
-
The Archean origin of assimilatory sulfate metabolisms provides novel insight into redox conditions of early Earth environments
… pathway exists, which starts with sulfate adenylyltransferase (sat/ATP sulfurylase) catalyzing a reaction of adenosine triphosphate (ATP) and sulfate (SO42-) resulting in adenosine 5’-phosphosulfate (APS), and incorporation of more reduced sulfur into biomolecules. This sat/ATP sulfurylase …
-
Properties of NadV / NatV Proteins in a Pyridine Nucleotide (NAD+) Scavenging System Encoded by Vibriophage KVP40
… domains, where the N-terminal region is a NMN adenylyltransferase (NMN ATase) converting NMN to NAD+, and the C-terminal region is a nudix hydrolase. NadR / PnuC and Sir2 are predicted to be NAm / NMN membrane transporters and to degrade NAD+, repectively. The purpose of this project was to …
-
Structural and Kinetic Characterization of Protein Ampylation by VopS Fic Domain
… by known AMPylators including Fic domain and adenylyltransferase domain proteins. Further, we showed that N6pATP can be used for the detection and purification of endogenous AMPylated proteins. Preliminary studies were performed on another effector protein of unknown function, VopQ from Vibrio …
-
Studies on pyridine nucleotide-dependent processes in Haemophilus influenzae
… these pathogens. Haemophilus influenzae ATP:NMN adenylyltransferase was partially purified 15-fold with a 27% yield using dye affinity chromatography. Affinity chromatography was also used to purify NAD kinase from Haemophilus influenzae, 18-fold with a 32% yield. Substrate specificity studies of …
-
The role of the RNA-binding protein FUS in axonal organisation and disease
… survival factor nicotinamide mononucleotide adenylyltransferase 2 (NMNAT2), as its mRNA is a potential FUS binding target. I hypothesised that axonal NMNAT2 levels are sustained by LPS, as this protein is known to have a very short half-life. However, by combining in situ hybridisation and …
-
Charakterisierung von NadR : das essentielle Enzym der NAD-Synthese bei Haemophilus influenzae
… als auch eine Nikotinamid-Mononukleotid-Adenylyltransferase (NMNAT) Aktivität in vitro gezeigt werden. Die Kristallstruktur von hiNadR im Komplex mit NAD+ wurde auch aufgeklärt. In dieser Arbeit sollte NadR, insbesondere dessen RNK Domäne, in vivo und in vitro näher charakterisiert …
-
Metabolic Regulation of Transcription Through Compartmentalized NAD+ Biosynthesis
Extracellular signaling and nutrient availability are major factors for the cell fate decision. Responds to extracellular information requires metabolic alterations and differential gene expression. Recently emerging concept of metabolic regulation of transcription reveals that fluctuation of …
-
Investigation of storage polysaccharide metabolism in lactic acid bacteria
… (ADP-Glc-PPase; ATP: α-d-glucose-1-Phosphat adenylyltransferase, EC 2.7.7.27), kodiert vom Gen glgC, gefolgt von den Reaktionen der Glykogen-Synthase (EC 2.4.1.21) und des Verzweigungsenzyms (EC 2.4.1.18), von dem die Genen glgA und glgB Genen kodiert sind. Der entscheidende …