Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"adenosine deaminase"”.
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Nuclease-Mediated Targeted Gene Insertion at the Adenosine Deaminase Locus in Primary Cells
… severe combined immunodeficiency (SCID) due to adenosine deaminase (ADA) deficiency. Initial trials looked promising and the technique was extended to other forms of primary immunodeficiency. Unfortunately, some of the patients enrolled in these trials using retroviral vectors to carry …
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Structure Function Analysis of Adenosine Deaminase Acting on tRNA (ADAT2) from Trypanosoma Brucei
… code. The enzymes responsible for deamination of adenosine 34 to inosine are found in all organisms and in all but a few cases are essential for life. In bacteria the reaction is carried out by the homodimeric adenosine deaminase acting on tRNA (ADAT) and modifies only tRNAarg, whereas in eukarya …
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The diagnostic value of adenosine deaminase activity in the ascitic fluid of patients with tuberculous peritonitis
… large number of patients, 3. Establish levels of adenosine deaminase activity which give the best discriminatory information in patients with ascites. 4. Determine what conditions may give rise to false positive or false negative results. 5. Finally, the study was designed to assess the relative …
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Utility of chloride and adenosine deaminase measurement in cerebrosphinal fluid for the early presumptive diagnosis of tuberculous meningitis
Background: Chloride and adenosine deaminase measurements in cerebrospinal fluid are still sporadically requested as part of tuberculous meningitis work-up. In the literature, evidence is contradictory and opinion is divided on their utility in clinical practice. The accuracy of both for the early …
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Tuberculous pleural effusions : a prospective study of rapid diagnostic tests (adenosine deaminase, antigen capture enzyme-linked immunosorbent assay, and the polymerase chain reaction) and evaluation of a radiometric mycobacterial culture system
… diagnostic tests assessed on pleural fluid were adenosine deaminase (ADA), an antigen (BCG) capture enzymelinked immunosorbent assay (ELISA), and a specific DNA probe after amplification with the polymerase chain reaction. ADA was found to have a sensitivity of 0.77 and a specificity of 0.83 in …
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Biochemical Studies Of Human Hyperuricaemia
… to be linked. A significant increase in serum adenosine deaminase (3.5.4.4.) activities has been found in a heterogeneous hyperuricaemic group compared with controls. Electrophoretic studies of erythrocyte phosphoribosylpyrophosphate synthetase, adenosine deaminase, hypoxanthineguanine …
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Syntheses of Dimensionally Altered Enzyme Substrates by Means of the Finkelstein Reaction (Benzopurine, Naphthopurine)
… (lin-naphthoadenosine) have been developed. The synthetic route commenced with 5,6-dimethylbenzimidazole and the linear array of rings was constructed by means of the Finkelstein reaction. lin-Naphthoadenosine was synthesized by ribosidation of …
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The Adar-Mavs Pathway Is A Critical Mediator of The Innate Immune System In Pancreatic Development and Cancer
<p>Adenosine deaminase acting on RNA (ADAR) is an RNA-binding protein that deaminates adenosine (A) to inosine (I). A-to-I editing is an important post-transcriptional mechanism to prevent recognition of endogenous RNA by MDA5, a cytosolic RNA sensor. Activation of MDA5 by viral RNA can stimulate …
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The Significance of Cleaved Cytokeratin 18 in Pleural effusion
… = 0.52, p < 0.05), 총 단백질 (rs = 0.45, p < 0.05), adenosine deaminase (rs = 0.38; p < 0.05) 등과 유의한 상관관계를 보였다. Receiver operating characteristics (ROC) 분석 결과 결핵성 흉수의 AUC는 0.933이였다. M30에 대한 면역조직화학염색 결과 폐암의 흉막조직에서는 종양세포에서 중등도의 발현이 관찰되었고 결핵성 흉막 조직에서는 육아종 내 상피양세포에서 높은 발현이 관찰되었다. 결론 : 결핵성 흉수에서 상피세포의 사멸이 …
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Identification and Characterization of Novel CRISPR-Cas Immune Effectors
… that were active in our CRISPR system, a CARF-adenosine deaminase protein fusion called CRISPR-associated adenosine deaminase 1 (Cad1), which deaminates ATP to ITP upon cOA binding and a CARFTIR protein fusion called CRISPR-associated TIR protein 1 (Cat1), which hydorlyzes NAD<sup>+</sup> …
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The Role of A2B Adenosine Receptor Signaling In Adenosine Dependent Lung Disease
… result in significant morbidity and mortality. Adenosine is a signaling nucleoside generated in response to injury and can serve both protective and destructive functions in tissues and cells through interaction with four G-protein coupled adenosine receptors: A1R, A2AR, A2BR, and A3R. However, …
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Integrated modulation of sympathetic tone in the microcirculation by oxygen, adenosine, and nitric oxide
… project, we sought to test the hypothesis that adenosine, formed in response to a flow-dependent fall in local PO2, serves as the stimulus to preserve endothelial NO release under these conditions. Sympathetic nerve stimulation induced frequency-dependent arteriolar constrictions and a …
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Metabolism in Vascular Endothelial Cells: Effects of Hyperglycaemia, Pro-Inflammatory Cytokines and Polyphenols
… of the anti-inflammatory metabolites adenosine and inosine. Subsequently, quercetin metabolites inside the cells and in the culture medium after quercetin treatments were identified, and their effects on the activities of enzymes involved in purine metabolism enzymes were investigated. …
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Aetiology and presentation of childhood pleural infections in the post-pneumococcal conjugate vaccine era in South Africa
… dehydrogenase (7280 vs 544 U/L, p< 0.001), and adenosine deaminase levels (162 vs 48 U/L, p< 0.001) and lower glucose levels (1.3 vs 4 mmol/L, p< 0.001). Conclusion Post-PCV, S. aureus is the dominant cause of PE in children using traditional culture methods, while TB remains a common cause of …
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Biochemical and Structural Studies of RNA and DNA Editing Enzymes
… of hydrolytic deamination of cytidine (C) and adenosine (A), is a modification event that profoundly effects on an organisms vitality and quality of life. This study focuses on two types of enzymes---ADAT (adenosine deaminase acting on tRNA) and APOBEC3G (apolipoprotein B editing catalytic …
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Reading Between the Strands: Deciphering Double Stranded RNA-Based Immune Responses in Mosquito-Virus Interactions
… signaling network. Finally, we investigated adenosine deaminase acting on RNA (ADAR), a conserved dsRNA-binding protein. Using both knockdown and overexpression approaches, we found that each reduced virus replication and dampened immune gene expression, suggesting that ADAR levels must be …
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Newer and novel sputum versus non-sputum-based tools for the diagnosis of active tuberculosis in different patient sub-populations
… other same-day tests including Xpert Ultra and adenosine deaminase (ADA) in 99 South African patients with suspected pericardial TB using a composite reference standard including pericardial fluid, pericardial tissue TB culture, pericardial tissue histopathology and response to TB treatment. …
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Elevated Cochlear Adenosine Causes Hearing Loss Via Adora2B Signaling
… metabolic energy currency. Patients who lack adenosine deaminase (ADA), the enzyme that irreversibly metabolizes adenosine, have high levels of adenosine that yield severe health problems, including HL; however, the pathogenic mechanisms behind HL and adenosine remain elusive. Our lab has …
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The Role of Il-6 In Adenosine-Mediated Pulmonary Fibrosis
<p> <p>Adenosine is a purinergic signaling molecule that regulates various aspects of inflammation and has been implicated in the pathogenesis of chronic lung diseases. Previous studies have demonstrated that adenosine up-regulates IL-6 production through the engagement of the A<sub>2B</sub> …
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