Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 79 for “"adaptor proteins"”.
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Phase Transitions of Multivalent Adaptor Proteins
… are self-assembled entities of lipids, proteins and carbohydrates. Additionally, within and on surface of membranes, molecules cluster into signaling compartments in many different biological pathways. Interactions between individual biomolecules have been studied comprehensively in …
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Role of Adaptor Proteins in MPR sorting
… Transport der Rezeptoren erfordert die monomeren Adaptor-Proteine GGA und den heterotetrameren Adaptor-Protein Komplex AP-1. Wir generierten und analysierten chimere Proteine der Rezeptor-bindenden Untereinheit µ1 des AP-1 und der homologen µ2 Untereinheit des AP-2 Komplexes der Plasmamembran und …
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Binding properties of adaptor proteins Tollip and Tom1
Adaptor proteins, like Tollip and Tom1, facilitate cellular cargo sorting through their ubiquitin-binding domains. Tollip and Tom1 bind to each other through their TBD and GAT domains, respectively, whereas Tollip interacts with phosphatidylinositol-3-phosphate (PtdIns(3)P)-containing endosomal …
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The role of adaptor proteins Crk and CrkL in lens development
… fiber cell elongation have not been identified. Adaptor proteins Crk and CrkL are SH2- and SH3-containing proteins that transduce signals from upstream tyrosine phosphorylated proteins to downstream effectors, including Ras, Rac1 and Rap1, which are important for cell proliferation, adhesion and …
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The Role of Adaptor Proteins in rDNA Double-Strand Break Repair by Homologous Recombination
… rDNA breaks repair by HR. I describe that the adaptor proteins Treacle and MDC1 play a central role in coordinating the sequence of events that ensue in response to rDNA DSBs. Firstly, the phosphorylation of the histone variant H2AX by the ATM kinase is dependent on Treacle and its two …
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Comparison of CLEC-2 and GPVI signaling in platelets: the role of adaptor proteins
… pathway and also exclude the LAT-family proteins PAG, LIME, LAX and NTAL as potential LAT replacements in platelet activation by GPVI. These results extend our understanding of platelet activation by CLEC-2.
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Characterization of signaling pathways regulating nephrin endocytosis in kidney podocytes: novel roles for Nck and ShcA adaptor proteins
… between nephrin and Nck, a cytoskeletal adaptor. Targeted genetic disruption of nephrin/Nck interactions further exacerbates disease in mice, verifying a crucial role for nephrin/Nck signaling in withstanding insult. We next characterized a fundamental role for Nck in mediating …
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Klonierung der cDNA des Protein A Kinase-Adaptor-Proteins-2 und Untersuchungen zur Regulation seiner mRNA in humanen fötalen Osteoblasten
… cDNA-Sequenz des Proteinkinase A-Ankerproteins-2 (AKAP-2, 7,5kB) ermittelt. Zu diesem neuen humanen Gen sind bis dato außer einem unvollständigem Datenbankeintrag noch keine experimentellen Daten zur cDNA, mRNA oder zum Protein veröffentlicht. Mittels Northernblot wurde die Regulation …
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Specificity and regulation of substrate degradation for a AAA+ protease
… enzymes degrade hundreds of different proteins. Determining how the activities of these proteases are regulated in the cell as well as learning how these enzymes bind and engage substrates are important goals. In order to better understand how the degradation of ClpXP and ClpAP is …
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Insights into the role and mechanism of the AAA+ adaptor Clps
… with respect to substrate selectivity. Adaptor proteins that can associate with the proteases add an additional layer of control to substrate selection. Thus, understanding the mechanism and role of adaptor proteins is a critical component to understanding how proteases choose their …
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Mechanism of active substrate delivery by the AAA+ protease adaptor CIpS
… substrates into an associated peptidase barrel. Adaptor proteins assist in recognition and degradation of certain substrates, but how enzyme-adaptor pairs ensure proper substrate selection is incompletely understood. In this thesis I focus on the delivery mechanism employed by the bacterial …
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Tunable Microchips for Imaging Protein Structures formed in Breast Cancer Cells
… cancer cells. These microchips are coated with adaptor proteins that include antibodies against target proteins of interest. The adaptor proteins are added in sequential steps to the coated microchips, followed by an aliquot of sample containing the protein of interest, such as BRCA1. The Kelly …
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Structural basis of membrane targeting and regulation of the innate immunity adaptor TIRAP by its phosphoinositide-binding motif
… trigger a cytoplasmic signaling cascade through adaptor proteins, with the first being the TIR domain-containing adaptor protein (TIRAP). TIRAP contains a TIR domain, which associates with TLRs and other adaptor proteins; and a N-terminal phosphoinositide-binding motif (PBM) that mediates the …
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The characterization of adaptor protein homologues in Plasmodium falciparum
… in the normal processes of parasite metabolism. Adaptor protein complexes are components of protein coats that associate with transport vesicles of the endocytic and secretory pathways in mammalian cells. Homologues of several adaptor protein subunits are encoded by the parasite genome. The …
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Substrate selection by the ClpXP protease : a tail of destruction
… modulation of proteolysis of ssrA-tagged proteins. Finally, additional substrates whose degradation may be regulated by the adaptor protein SspB were determined by identifying substrates captured in ClpXP[trap] in an sspB⁺ strain but not an sspB⁻ strain. This analysis led to the …
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WNK Family Kinases and the Regulation of Ion Flux
… regulate the activity of a diverse group of proteins that in toto suggest WNKs are involved in ionic homeostasis by means of constitutively regulated endocytosis of ion transporters and channels. The mechanism is reliant on an amino terminal fragment of WNK that does not include the kinase …
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Roles for C-ABL and P53 in bone homeostasis and DNA damage response
… MEFs showed defects in nuclear foci assembly of adaptor proteins such as TopBP1, Brca1, activation of Chk2 and Chk1, phosphorylation of p53 at Ser15 and induction of p53 target genes, cell cycle checkpoints and DNA repair of ssDNA breaks. These findings were confirmed by c-Abl reconstitution. …
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Expression and Function of Toll-Like Receptor 2 and 4 in Human Melanocytes
… the mRNA expression of TLR2, TLR4 and their adaptor proteins, CD14 and myeloid differentiation factor-88 (MyD88) was shown by RT-PCR. Protein expression and localization of TLR2, TLR4 and CD14 were detected by western blot, flow cytometry and immunocytochemistry. Lipopolysaccharide (LPS) was …
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Determining the effects of phosphorylation on AFAP-110 function
… potential 14-3-3 binding sequences. 14-3-3 proteins are a family of small acidic adaptor proteins of dimeric nature that act as molecular scaffolds and/or allosteric regulators in a number of cellular pathways. We have been interested to determine whether 14-3-3 is a binding partner for …
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