Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"acyl-ACP"”.
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Studies of Lipoic Acid Related Enzymes
… screening, I also isolated the Vibrio harveyi acyl-ACP synthetase gene and showed that it encoded a 60.4 KDa protein that shared high homology with medium chain fatty acid-CoA ligases. I expressed the His-tagged protein at a high level in E. coli and purified it to homogeneity. To explore the …
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Determination of fatty acid synthesis intermediates in escherichia coli and bacillus subtilis
… A key feature of the FAS pathway is the acyl carrier protein (ACP), which is a small acidic protein that tethers acyl intermediates via a high-energy thioester bond and shuttles them between enzymes. In Escherichia coli, ACP is one of the most abundant soluble proteins with about 60000 …
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UNCOVERING THE MOLECULAR BASIS FOR FATTY ACID SYNTHASE PRODUCT SPECIFICITY: STRUCTURAL AND KINETIC EVALUATION OF THIOESTERASE I AND THIOESTERASE II
… strict product distribution is dictated by the acyl chain-length selectivity of the endogenous thioesterase (TE1) domain. With the exception of lactating breast, liver and adipose tissues, FASN expression is essentially absent in normal cells since these cells obtain lipids from the diet. In …
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Palmitoyl-acyl Carrier Protein Thioesterase in Cotton (Gossypium hirsutum L.): Biochemical and Molecular Characterization of a Major Mechanism for the Regulation of Palmitic Acid Content
… be due in part to the activity of a palmitoyl-acyl carrier protein (ACP) thioesterase (PATE). In embryo extracts, PATE activity was highest at the maximum rate of reserve accumulation (oil and protein). The cotton FatB mRNA transcript abundance also peaked during this developmental stage, …
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Metabolic engineering of microorganisms for the overproduction of fatty acids
… prioritization of interventions, fabZ and acyl-ACP thioesterase were upregulated and fadD was deleted to arrive at a strain that produces 1.70 g/L and 0.14 g fatty acid/g glucose of C14-16 fatty acid in minimal medium. However, OptForce does not infer gene regulation, enzyme inhibition and …