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Showing 1 to 2 of 2 for “"active-site lid"”.

  1. Structure-Function Studies on Two Phosphoenolpyruvate Carboxylases

    … reaction mechanism. Both operate through a lid-gated active site and have a hypothesized enol-pyruvate intermediate in their catalytic pathway. While PEPCK is an important metabolic enzyme in animals and plays a broad role in cataplerosis, gluconeogenesis and glyceroneogenesis, Pepc reaction …

    vt Repository record for Structure-Function Studies on Two Phosphoenolpyruvate Carboxylases (opens in a new tab)

  2. Structural and functional studies of phosphoenolpyruvate carboxykinase

    … which results in it being ‘pushed’ towards the active site to react. Previous kinetic studies on the E. coli PCK mutant Lys213Ser have determined that Mn2+ is unexpectedly inhibitory. A crystal structure of K213S-PCK-ATP-Mg2+-Mn2+ demonstrates that Mn2+ is tetrahedrally coordinated in the active

    sask Repository record for Structural and functional studies of phosphoenolpyruvate carboxykinase (opens in a new tab)