Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"activation domains"”.
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Eukaryotic Transcriptional Activation Mechanism: Protein-Protein Interactions
… I analyzed the function of distinct kinds of activation domains in yeast and human. The results showed that the proline-rich activation domain of CTF1 can, like acidic activation domains, activate transcription in yeast and human. Based on this, I compared the activation pathways by acidic and …
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Characterization of Transcription Factor-Coactivator Complexes in Acute Lymphoblastic Leukemia
… translocation 1;19. E2A-PBX1 contains the activation domains of E2A and most of the PBX1 protein, including the DNA-binding homeodomain. These structural features suggest a hypothetical oncogenic model in which E2A-PBX1 binds DNA, recruits transcriptional co-activators by means of the …
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Detailed Analysis of Transcriptional Regulation by Activation Functions-1 and -2 of the Human Estrogen Receptor
… protein that can be divided into separable domains with specific functions, such as ligand binding, dimerization, DNA binding, and transactivation. The ER contains two distinct activation domains that are involved in transcription activation. Activation function-1 (AF-1) is located in the …
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Detection of Polypeptide Interactions Via Periodate Triggered Dopa Crosslinking
… interactions between the 26S proteasome and activation domains as well as the Arp 2/3 complex and the CA peptide. Finally, we present the creation of a chimeric molecule consisting of the biarsencial fluorescent reporter FLAsH conjugated to 3,4-Dihydroxyphenylalanine as a route to deliver …
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Characterization of a Putative Activation Domain in the Hulk Gene Family
… To investigate the putative transcriptional activation domain in the carboxyl-terminus of the HULKs, full-length HULKs and deletion constructs were 3-AT titrated in yeast-one hybrids. It was found that the transcriptional activity varied between both the full-length and carboxyl-terminus of …
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Hsp70-Mediated Regulation of Hsf1 Transcriptional Activity In Saccharomyces Cerevisiae
… CE2 within the carboxyl-terminal transcriptional activation domain, and one within the amino-terminal activation domain. Disruption of the identified <em>in silico</em> Hsp70-binding sites within each regulatory element results in loss of Ssa1 association. Furthermore, loss of regulation by Ssa1 …
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Highly efficient Cas9 mediated transcriptional programming and delivery via Adeno-Associated Virus
… the direct fusion of a synthetic transcriptional activation domain (AD). However, the modest levels of gene activation achieved by first generation Cas9 activators limited their potential applications. We describe the development of a versatile, improved transcriptional regulator through the …
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Molecular Mechanisms of C11orf95 translocations in cancer.
… transcriptional signature mediated by the trans-activation domains (TAD) of the fused c-terminal protein partner. Specifically, the ZF’s of C11orf95 direct DNA-binding (as shown with ChIP-seq) of the fused protein partner but does not appear to alter the c-terminal protein’s nuclear interactome …
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Batf regulates previously unknown AP-1 target genes to control TH17 differentiation
… proteins also contain defined transcriptional activation domains: TADs), some consist only of a basic region and leucine zipper and are thought to function as inhibitors of AP-1 activity. We found that the AP-1 protein Batf, which lacks a TAD, is highly expressed in T helper cells compared to …
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Controlling Gene Expression with Synthetic Molecules
… factors are minimally composed of two domains, a DNA-binding domain (DBD) and an activation domain (AD). Several synthetic DBDs capable of recognizing DNA in a sequence specific manner have been reported in the literature. Furthermore, studies have demonstrated that coupling of these …
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Car-Modified T Cells Capable of Distinguishing Normal Cells From Malignant Cells
… specificity derived from an antibody to T-cell activation domains to result in lysis of TAA-expressing cells. Due to the potential for on-target, off-tissue toxicity, CAR<sup>+</sup> T-cell therapy is currently limited to unique or lineage-restricted TAAs. Glioblastoma, a grade IV brain …
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Understanding C/EBPbeta LAP/LIP Transcriptional and Adipogenic Potential Through Regulation by HDAC1 and GCN5
… a repressor of transcription due to its lack of activation domains. Numerous studies have shown that C/EBPβ acetylation state modulates its activity in a promoter-specific manner. For instance, the acetyltransferases GCN5/PCAF and the deacetylase complex mSin3A/HDAC1 regulate C/EBPβ activity on …