Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 16 of 16 for “"actin remodeling"”.
-
Actin remodeling in motile cells
… on the dynamics and distributions of cytoplasmic actin. In cells, actin cycles between monomeric and polymeric phases tightly regulated by actin binding proteins that control cellular architecture and movement. Here, we characterize actin remodeling in shear stress stimulated endothelial cells and …
-
Bitesize bundles F-actin and influences actin remodeling in syncytial Drosophila embryo development
Actin networks undergo rearrangements that influence cell and tissue shape. Actin assembly and organization is regulated in space and time by a host of actin binding proteins. Despite the importance of these processes, their regulation is not well understood. The Drosophila Synaptotagmin-like …
-
How short, degenerate motifs across the human proteome recognize the actin remodeling factor ENAH
Protein-protein interactions form the backbone of the myriad signaling pathways that cells use to respond to their environments. A central class of protein-protein interactions in eukaryotic interactomes are those that occur between short linear motifs (SLiMs) and structured domains. SLiMs are …
-
NOVEL MECHANISMS IN THE REGULATION OF PERINUCLEAR ACTIN ASSEMBLY
Proper organization and dynamics of actin cytoskeleton are critical for cells? functions and survival. Perinuclear actin contributes to maintaining of nuclear shape and cellular mechanical homeostasis, and integrating cell nucleus into the actin cytoskeleton architecture. Actin dynamics is …
-
Regulation of Exocytosis by Syntaxin 4-Munc18c Complexes
… cells. Syn4-mediated insulin release requires F-actin remodeling to mobilize insulin granules to the plasma membrane. Our studies reveal that Syn4 directly associates with F-actin in MIN6 beta cells, and that the disruption of this complex correlates with increases in glucose-stimulated insulin …
-
Effect of oncomodulin on cytoskeletal actin dynamics.
… to sound. Regulation of filamentous and globular actin may contribute to the reversible shape changes critical to OHC function. Previous studies have suggested that the Rho family of GTPases play an important role in regulating intracellular actin dynamics in OHCs using RhoA/ROCK and LIMK/cofilin …
-
The Effect of Microenvironmental Cues on Adipocyte Cytoskeletal Remodeling
… on the adipocyte mechanical response, including actin cytoskeletal remodeling. This dissertation aims to develop an in vitro model of healthy/diseased AT to explore the effect of microenvironmental cues on adipocyte function and actin cytoskeletal remodeling. The first aim is to study (1) the …
-
Role Of Gravin In Endothelial Wound Healing
… subunits, PKCα, PDE4, β2 adrenoreceptor and actin cytoskeleton. Initial characterization of gravin has revealed that this protein is undetectable in endothelia in vivo but expressed at the periphery of cultured endothelial cell (EC). Although the precise role of gravin in human ECs is not yet …
-
MECHANO-CHEMICAL FEEDBACK VIA CONTACT PERCOLATION REGULATES PHASE TRANSITION AND TRANSCRIPTIONAL STATE IN HETEROGENEOUS BREAST CANCER
… by lamellipodia formation and RAB5A-dependent actin remodeling in control cells. In both 2D and 3D models, contact-driven percolation triggers a pro-inflammatory transcriptional switch in non-RAB5A cells, mediated by secreted factors and direct physical interactions. Our findings establish …
-
Molecular Mechanisms of Melatonin Action on the Rat Suprachiasmatic Nucleus: An in Vitro Analysis
… melatonin on Per1 and Per2 are mediated by cis-acting regulatory enhancer sequences termed E-boxes in the promoters of these dock genes. Finally, transient rearrangement of the actin cytoskeleton plays a crucial role in conveying the melatonin signal from the plasma membrane into the cell to …
-
Protein Regulators of Phosphoinositides as Promoters of Cancer Metastasis
… cytoplasm. Cytoplasmic cofilin binds and severs actin, generating free barbed ends in actin filaments and inducing actin polymerization. PTPRN2 and PLCβ1-mediated actin remodeling dynamics increasecellular migration, a key metastatic phenotype.</p>
-
Identification and Characterization of Novel Mechanisms of Functional and Structural Synapse Remodeling: Focus on Vav Guanine Nucleotide Exchange Factors and MEF2 Transcription Factors
… the stimulation of Rho GTPases and subsequent F-actin remodeling and dendritic spine structural dynamics. I report that Vav-family GEFs are activated by BDNF/TrkB signaling, and are required for BDNF-induced Rac-GTP formation. Vav GEFs, which are enriched at hippocampal glutamatergic synapses, …
-
Rho GTPase family members in establishment of polarity in C. elegans embryos
… (PAR) proteins as well as the actin cytoskeleton. In Caenorhabditis elegans one-cell embryos, the PAR proteins are segregated into an anterior (PAR-3, PAR-6) and a posterior (PAR-1, PAR-2) corticaldomain. The formation of PAR polarity correlates with anterior-posterior …
-
Endosomal membrane dynamics underlying cell spreading: A role for the small GTPase Arf6
… process is critical to the development of counteracting therapeutics. Cell migration and adhesion-dependent cell spreading share a number of features. For example, both processes rely on the activation of mechanisms for the coordinated spatial and temporal assembly/disassembly of focal adhesions, …
-
Actin's role in signaling light-induced phase shifts in the mammalian circadian clock
The actin cytoskeleton is a critical part of intracellular structure, responsible for the general shape and integrity of all cells. New insights into the dynamic nature of the cytoskeleton describe additional roles for actin, including synaptic plasticity in the hippocampus, regulation of hormone …
-
Cytoskeletal Networks Driving Presynaptic Plasticity
… cascades that ultimately expand or contract actin networks within postsynaptic sites. In this dissertation, I advance the field of synaptic plasticity by focusing on presynaptic terminals, which are equal partners with their postsynaptic counterparts. To date, the study of presynaptic …