Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 39 for “"actin binding proteins"”.
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Identification of Actin Binding Proteins Associated with Cross-Linked Actin Networks
Mills, Christy E., Identification of Actin Binding Proteins Associated with Cross-Lined Actin Networks. Master of Science (Pharmacology and Neuroscience), December 2006, 95 pp., 9 tables, 16 figures, references, 122 titles. Glucocorticoid therapy can leady to ocular hypertension and glaucoma. The …
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Functional studies of selected actin binding proteins by point mutations and GFP fusions
… concomitant with a slight reduction in actin-binding, whereas the K114E profilin shows profound decrease in its ability to interact with actin but its affinity for poly-(L)-proline remained unaltered. The in vivo properties of the point-mutated profilins were studied by expressing either …
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Mapping the actin and actin binding proteins interactions : from micromechanics to single molecule force spectroscopy
… models describing cell behavior. Filamentous actin (F-actin), as one of the major constituents of the cytoskeleton, has been the target of extensive in vitro studies to determine its mechanical properties in bulk. However, there is still a lack in the understanding of how the molecular …
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Multi-Modal Regualtion of Actin Networks
<p>Eukaryotic cells employ the actin cytoskeleton to maintain cell shape, support motility, and sense and respond to external mechanical stimuli. An intricate network of actin filaments constitutes the foundation of tissue architecture by forming key cellular structures including the muscle fiber, …
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Combined optical trapping and single molecule fluorescence to study the force-dependent binding kinetics between filamentous actin and its partners
Actin filaments are a major constituent of the cytoskeleton in most eukaryotic cells. They function as a connection between the cell body to the focal adhesions in order to transmit forces into and out of the cell. During the force transduction process, many proteins bind to actin filaments in …
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Motility and Force Generation Based on the Dynamics of Actin Gels
This thesis explores the force generation of actin gels and their famous pro-pulsion mechanism leading to active motility on the cellular scale. In nature the generation of force and motility is regulated by actin binding proteins and the mesoscopic shape of the gel. Here we controlled an analyzed …
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P21- ACTIVATED KINASE 4 AND ACTIN CYTOSKELETON DYNAMICS
… THE GROUP II PAK FAMILY KINASES, IS ONE OF MANY ACTIN DYNAMICS REGULATORS THAT CONTROL THE FORMATION OF ACTIN-RICH STRUCTURES SUCH AS FILOPODIA IN MAMMALIAN CELLS. PAK4 PHOSPHORYLATES LIM KINASE (LIMK), WHICH IN TURN, PHOSPHORYLATES THE ACTIN DEPOLYMERISATION FACTOR, COFILIN. COFILIN …
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Calpain 9 Functions in TNF Receptor Mediated Apoptosis
… cancer development. The Inhibitor of Apoptosis Proteins, IAPs, block Caspase activity and cell death. Release of the Second Mitochondria-Derived Activator of Caspases, Smac, from the mitochondria relieves IAP Caspase inhibition, activating apoptosis. Our lab has developed a small molecule Smac …
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Inactivation of Tor Proteins Affects the Dynamics of Endocytic Proteins in Early Stage of Endocytosis
… Since the recruitment of endocytic proteins such as actin binding proteins and the amphiphysins precedes the internalization of α-factor, I hypothesized that loss of Tor function leads to an alteration in the dynamics of the endocytic proteins. I report here that endocytic proteins, …
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Bitesize bundles F-actin and influences actin remodeling in syncytial Drosophila embryo development
Actin networks undergo rearrangements that influence cell and tissue shape. Actin assembly and organization is regulated in space and time by a host of actin binding proteins. Despite the importance of these processes, their regulation is not well understood. The Drosophila Synaptotagmin-like …
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Molecular and Cellular Mechanisms of LIM Protein Mechanotransduction
… of mechanosensation, the mechanisms by which proteins convert mechanical stimuli into biochemical signals (mechanotransduction) are poorly understood. The actin cytoskeleton serves as a nexus for cellular mechanotransduction, generating and transducing forces into intracellular signals that …
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Mechanical properties of F-actin network
… surroundings through cytoskeletal dynamics. Actin, the most abundant protein found in eukaryotic cells, is organized into various cytoskeletal structures that provide physical support for the cell and play important roles in numerous cellular processes. Assembly of F-actin into higher-order …
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Tyrosine Phosphorylation of Villin: Effects on Actin Dynamics, Cell Morphology and Cell Migration
… study we demonstrate that an epithelial cell actin-binding protein, villin, plays a crucial role in the process of cell migration. Overexpression of villin in doxycyline-regulated HeLa Tet-off and MDCK Tet-off cells enhanced cell migration. We further demonstrate that tyrosine phosphorylation …
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Microstructurally-based constitutive models of cytoskeletal networks for simulation of the biomechanical response of biological cells
… rheological data of bundled/unbundled in vitro F-actin networks. The cytoskeletal network model has also been extended to include the internal energy based mechanical contributions at the filament and network levels from torsional crosslink deformations as well as from direct axial stretching of …
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Actin remodeling in motile cells
… on the dynamics and distributions of cytoplasmic actin. In cells, actin cycles between monomeric and polymeric phases tightly regulated by actin binding proteins that control cellular architecture and movement. Here, we characterize actin remodeling in shear stress stimulated endothelial cells and …
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Regulation of the actin cytoskeleton in the neuronal growth cone
… I have examined the roles of several known actin- binding proteins in the remodeling of the growth cone cytoskeleton. The Arp2/3 protein complex binds to an existing actin filament and nucleates formation of a daughter branch. Arp2/3 activity generates a dense actin network in fibroblast …
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The Interaction of Cofilin with the Actin Filament
The regulation of filamentous actin: F-actin) production from the polymerization of globular actin: G-actin) within the cell is critical for many cell functions. Since actin is found in all cells, understanding how actin-binding-proteins: ABPs) bind and how their regulating mechanisms work is not …
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Computational study of actin morphology and rheology
The cytoskeletal network consisting mainly of actin and actin binding proteins is highly dynamic, provides structural integrity to cells, and plays a central role in a wide range of mechanical and biological functions such as migration and the sensation of external forces. Thus, knowledge of actin …
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A Molecular Basis for Divergent, Unstable Actin Filaments in Toxoplasma gondii
… that is dependent on polymerization of parasite actin. However, in non-motile parasites, the majority of actin is monomeric and filaments only assemble upon initiation of gliding motility. Actin filament turnover is crucial for motility as shown by the detrimental effects of jasplakinolide, an …
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