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Showing 1 to 20 of 48 for “"acrosome"”.
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Regulation of SNARE function during sperm capacitation to promote the acrosome reaction
… to develop a clearer understanding of how the acrosome reaction is regulated during capacitation so that the molecular defects that cause infertility can be identified. I hypothesized that capacitation alters sperm protein phosphorylation promoting soluble N-ethylmaleimide-sensitive factor …
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Molecular Interactions During Fertilization: Sperm -Egg Binding and Regulation of the Acrosome Reaction
… candidate receptor was not identified. The sperm acrosome reaction is an exocytotic event that is initiated when sperm bind to the zona and is required for zona penetration. During the acrosome reaction, the plasma membrane fuses with the outer acrosomal membrane and vesicle contents are released. …
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Re-localization of SNARE proteins in mouse sperm prior to the acrosome reaction
… the outer acrosomal membrane. During the sperm acrosome reaction, the outer acrosomal membrane fuses at hundreds of points with the overlying plasma membrane, resulting in release of the acrosomal contents. We hypothesize that syntaxin and synaptobrevin re-localize within the sperm plasma …
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The Effects Of Equine Spermatozoa/Oocyte Co-Culture On Spermatozoal Acrosome Reaction And Viability
… of oocyte and spermatozoal interactions on acrosome reaction and viability of equine spermatozoa during in vitro fertilization. Three ejaculates were collected from three stallions on an every other day basis. Semen was processed and allowed to incubate in 37⁰ C with 5% CO₂ incubator for 15 …
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Identification of platelet activating factor (PAF) receptor in equine spermatozoa and its role in motility, capacitation and the acrosome reaction
… spermatozoal motility, capacitation, and the acrosome reaction (AR) were evaluated. Treatment of 10 stallion semen samples with 10 â 4 to 10 â 13 M PAF resulted in statistically significant differences in motility and capacitation (r2 = 0.81 and 0.83 respectively). The concentration of PAF, …
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Characterization of the Biological Functions of Human Recombinant Zona Pellucida Protein 3
… assay and immunofluorescence staining of acrosome reaction The results indicated that rhZP3 exhibited an inhibition in the binding assay (HZI 43.6 +/-3.3; n = 9; 30 ng/mL rhZP3) and induction of acrosome reaction (198.6% +/- 77.2% increase from baseline; n = 29; 30 ng/mL rhZP3). It was …
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Expression and Function of Complexin 1 in Mammalian Sperm Acrosomal Exocytosis
… exocytosis but the sperm vesicle, known as the acrosome, is a single large vesicle whose release has different characteristics than neuronal exocytosis. Upon adhesion to the mammalian egg extracellular matrix, the sperm acrosome is released to allow penetration of the egg coat. How vesicle …
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Material properties of actin filament bundles
… during spermatogenesis. In addition to actin the acrosome comprises only one other protein: scruin, an actin-binding protein specific to Limulus that decorates and crosslinks actin filaments into a crystalline bundle. Our goal is to reconstitute the structure of the acrosome using these two …
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Structural and functional aspects of sperm after transit through the fallopian tube of the sheep
… include the processes of capacitation and the acrosome reaction. The Fallopian tube has been accepted as the site of completion of these events while their initiation may occur either in the uterus or in the Fallopian tube. The role and/or interaction of the Fallopian tube with sperm to undergo …
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Choline Acetyltransferase and Carnitine Acetyltransferase Activity in Human Spermatozoa During Capacitation
… and pharmacological induction of the acrosome reaction. A significant increase in both equatorial and midpiece/tail immunoreactivity was observed when using both caffeine and pentoxifylline. The addition of caffeine significantly increased equatorial binding, midpiece/tail binding, and …
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Development and Characterization of Monoclonal Antisperm Antibodies: Potential for Contraception
… overlaying different regions of the sperm, i.e., acrosome, postacrosome, equatorial, midpiece, and tail. When these anti-sperm MAbs were tested on fresh, capacitated, and acrosome reacted spermatozoa, a differential reactivity with fresh sperm as compared to acrosome reacted sperm was observed. …
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Characterization of the Biological Activities of Recombinant Fusion Protein Green Fluorescent Protein/Human Zona Pellucida Protein 3 (GFP/HZP3)
… protein interacted with human sperm on the acrosome region. In the acrosome reaction assay, fusion protein was proved to be able to induce the sperm acrosome reaction (19.2% ± 3.4% as compared to basal line 10.8% ± 2.3%) at the concentration of 1ug/ml. Therefore, the fusion protein displayed …
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Determination of the effects of caffeine C1, 3. 7- trimethylxanthine in Tris-based egg yolk extender and different incubation intervals on the quality of cryopreserved bull semen
… motility, non-motile sperm cells, viability, acrosome integrity, and sperm cell morphological abnormalities using standard microscopic techniques. Semen for this study was collected using an electro-ejaculator and analysed using a computer-aided sperm cell analyser. Data were analysed using a …
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Expression, Isolation and Purification of Human Zona Pellucida Protein 3
… the subsequent triggering of the spermatozoa's acrosome reaction. Studies on the role of this specific protein in the human fertilization process are hampered by the limited amount of available biologically functional proteins.</p> <p>By use of a pcDNA 3.1(+) expression vector, a …
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The xenogenous capacitation response of fresh, cooled/extended and frozen/thawed equine semen as determined by a chlortetracycline stain
… of capacitated (PCAP), unreacted (PUR) and acrosome reacted (PAR) sperm. Mean PCAP was not significantly different in estrous ewes despite an increasing trend. Mean PUR and PAR were also not different. Differences in capacitated, acrosome reacted and unreacted spermatozoa in inseminated and …
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Sperm-egg interaction in birds: assays and mechanisms
… (FITC-PNA) was able to reliably detect acrosome-reacted chicken spermatozoa. PNA was found to bind to the exposed acrosomes of acrosome-reacted, but not acrosome-intact spermatozoa. At 40°C chicken spermatozoa co-incubated with IPVL required extracellular Ca2+ for induction of the …
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An investigation of the mechanics of an actin spring
… example is the dramatic process involving the acrosome reaction of Limulus sperm, whereby a 60-im long bundle of actin filaments straightens from a coiled conformation to extend out of the cell in five seconds. This cellular engine and the motion it produces represent a third type of …
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Localization of FC Receptors on Murine Spermatozoa.
… molecule and it can bind to both the acrosome and tail region of homologous and heterologous spermatozoa. Symons and Herbert (1971) characterized it as IgG in the mouse, while Johnson (1968) showed it to be IgG2 in the guinea pig. IgM and IgA do not show detectable binding to mammalian …
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3D localization of cytoskeleton in mouse spermatids using stochastic optical reconstruction microscopy
… in spermatogenesis. The development of the acrosome and the manchette, protein and vesicle transport in spermatids, and sperm head shaping are crucial steps in the formation of healthy sperms. The cytoskeleton in spermatids plays a crucial role in shaping the sperm head. The acroplaxome …
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