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Showing 1 to 20 of 36 for “"acridine orange"”.
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An evaluation of the theory and use of the acridine orange stain in enumerating bacterial populations
… pHs of aqueous solutions of the fluorochrome, acridine orange (AO). Results from this study gave strong supportive evidence for the non-vital staining properties of acridine orange contrary to previous reports in the literature. Green fluorescence of cells is not a reliable indicator of …
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A Study of the Coccoid Bodies of Prolinoboborus fasciculus (Aquaspirillum fasciculus)
… Although the coccoid form fluoresced with acridine orange, agarose gel electrophoresis revealed extensive ribosomal RNA degradation. Poly-Ã -hydroxybutyrate, abundant in the vegetative rods, was not detectable in the coccoid cells. The results suggest that the coccoid form of A. fasciculus …
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Kelvin Probe Force Microscopy on Graphene Thin Films for Solar Cell and Biosensing Applications
… cost-effective, water-soluble, and bio-sensitive acridine orange molecules. We investigated the morphology and work function of acridine orange using Atomic Force Microscopy and KPFM. Acridine orange’s ability to generate sufficient amounts of photo carriers was also demonstrated using KPFM under …
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Characterization of Commercial Pectin Preparations by Spectroscopic and Chromatographic Techniques.
… from local grocers. Purified pectin samples from orange peel, lemon peel, and apple pomace, each of low and high levels of methyl esterification and of unknown distribution pattern were also purchased. Instead of metal complexation, several highly absorbing dyes such as Ruthenium Red, Nile Blue, …
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Evaluation of Prebiotic Fibers and Pectin on Probiotic Strains and Effect of Bacteriocin-like Substance on the Survival of Listeria Monocytogenes
… In addition, we also aimed to explore the use of acridine orange as a method to monitor the growth response of bacteria. The antilisterial effects of Lactobacillus acidilactici are also studied using survival assays and spot-on-lawn assays.</p> <p>We determined that FOS, GOS, and IA are good …
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The Effects of Benzyl Alcohol on Developing Zebrafish (Danio rerio)
… studied.Cell death was observed using an acridine orange assay, showing a dramatic increase in cell death in a dose-dependent manner. While more work is needed to elucidate the effects of benzyl alcohol, these preliminary data suggest that there is a strong link between exposure to benzyl …
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Induction of Apoptosis and Necrosis By Infectious Pancreatic Necrosis Virus in Chinook Salmon Embryo Cells
… by epifluorescence microscopy using acridine orange and ethidium bromide and biochemically by 2% agarose gel electrophoresis and Hoescht 33256 fluorometric analysis of isolated DNA. Data indicate that CHSE-214 cells are capable undergoing spontaneour apoptosis. Furthermore, IPNV …
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Impact of delta-tocotrienol on human melanoma cell proliferation
… detected by fluorescence microscopy following acridine orange and ethidium bromide dual staining showed d-δ-tocotrienol-induced apoptosis in melanoma cells. Consequent to mevalonate deprivation and the putatively reduced prenylation and biological half-life of Ras protein, d-δ-tocotrienol …
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A surgical confocal microlaparoscope for real-time optical biopsies
… patients in vivo using uorescein sodium and acridine orange as the uorescent contrast agents. The results indicate that the device is safe and functions as designed. A Monte Carlo model was developed to characterize the system performance in a scattering media representative of human tissues. …
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Animal and Plant Viruses with Double-Stranded Ribonucleic Acid
… stains orthochromatically greenish-yellow with acridine orange, as if it contained DNA, yet it contains RNA; and 3. the multiplication of reovirus is inhibited by actinomycin D. Further evidence of the unusual characteristics of reovirus was obtained in studies of the synthesis of DNA, RNA, and …
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The Use And Development Of Laser Microdissection To Separate Spermatozoa From Epithelial Cells For Str Analysis
… methyl green, Wright's stain, and acridine orange. Hematoxylin/eosin out-performed all other stains however nuclear fast red/picroindigocarmine could be used satisfactorily with STR analysis. In addition, three DNA isolation methods were evaluated for LMD collected cells: QIAamp …
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Avaliação do potencial antileucêmico de adutos aromáticos de morita-baylis-hillman.
… studied using differential staining tests with acridine orange and ethidium bromide (LA/BE) probes, b eing observed under fluorescence microscope. The cell cycle and mitochondrial transmembrane potential analyzes were measured using the flow cytometry technique. Using the MTT assay, the compound …
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The Microbiological Aspects of Early Stages of Marine Fouling
… on surfaces were assessed and the use of an acridine orange staining method with UV-epiflourescence microscopy described. Various substrates representing a range of surface energy values (wettability) were immersed in seawater for different lengths of time during summer and winter seasons. …
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Effects of Scrotal Insulation on Spermatozoal Morphology and Chromatin Stability to Acid Denaturation in the Bovine
… denaturation via the metachromatic properties of acridine orange. SCSA is repeatable and has been used to distinguish between fertile and subfertile males in different species; however, it does not permit morphological evaluation of cells. In the present study, the SCSA was modified for the …
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The prevention and diagnosis of central venous catheter-related infection
… staphylococci in immunocompetent patients. The acridine orange leucocyte cytospin test (AOLC) was also evaluated for the rapid diagnosis of CR-BSI in 16 haematology/SCT patients with Hickman CVC in situ. Although the sensitivity of the test was low, it may provide a useful adjunct to …
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Some Physical and Chemical Properties of SALMONELLA POTSDAM Bacteriophages
… acid was DNA; no RNA was detected.</p> <p>The acridine orange test suggested a single stranded configuration of DNA.</p> <p>The molar contents of thymine and adenine were not equivalent, nor were the molar contents of guanine and cytosine.</p> <p>The phage DNA did not resemble in molar ratio of …
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Effect of bioxcell and triladyl extenders and removal of seminal plasma of equilibrated and cryopreserved goat semen
… using Spermac, chromatin structure using Acridine Orange, and mitochondria using JC-1 staining solutions. Both the Triladyl® and Bioxcell® washed semen groups were diluted (1:4) with Phosphate Buffered Saline (PBS) then centrifuged at 1500 x g for ten min and seminal plasma was aspirated …
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The effects of hydrology and vegetation on microbial community structure and soil function in the sediments of freshwater wetlands
… (16S-rDNA-based T-RFLP),bacterial abundance (Acridine Orange Direct Count), and soil function (Extracellular Enzyme Activity (EEA)). A mixed-effects repeated measures analysis of variance (ANOVA) was used to better understand how each variable responded within each hydrological regime and …
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