Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"aPKC"”.
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The Role of Atypical PKC in the Plasticity of the Circadian Clock in the Rat Suprachiasmatic Nucleus
… examined the role of atypical protein kinase C (aPKC) in GLU signaling. We found that the phosphotransferase activity of aPKC is increased significantly by GLU treatment to SCN brain slices at CT 14 in the early night. Disruption of aPKC phosphotransferase activity completely blocks GLU-induced …
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The Effects of Atypical Protein Kinase C on TGFβ Signalling
… and found that the Atypical PKC isoforms (aPKC; a subgroup of the PKC family) indeed can alter TGFβ receptor signaling. My work has shown that the modulation of aPKC expression or activity using inhibitors and/or small interfering RNA (siRNA) prolongs the temporal phosphorylation of the …
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Establishing and Maintaining Cortical Asymmetry in <i>Drosophila</i> Neural Stem Cells
… by the apically localised kinase, aPKC (atypical protein kinase C). This model proposes that aPKC localises to the apical cortex and phosphorylates Miranda, excluding it from the apical domain by phosphorylation of Miranda’s membrane binding motif. However, earlier work demonstrated …
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Function and Regulation of the Cell Fate Determinant Numb in Polarized Epithelial Cells
… Par complex protein, atypical Protein Kinase C (aPKC), as a means of regulating membrane localization and asymmetric distribution of Numb. A mutant of Numb that cannot be phosphorylated by aPKC accumulates on the plasma membrane and localizes to both apical and basolateral membranes. In …
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Functional analysis of the Bazooka protein in the establishment of cell polarity in Drosophila melanogaster
Für Komponenten des sogenannten PAR/aPKC- (partitioning-defective / atypische Proteinkinase C) Komplexes wurde nachgewiesen, dass sie eine Schlüsselrolle in der Entstehung und Erhaltung der Zellpolarität in unterschiedlichen Zelltypen spielen. Die grundlegenden Mechanismen scheinen hierbei in der …
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Functional Interactions Between Par-1b Protein Kinase and RNF41 E3 Ubiquitin Ligase
… C: PKC) pathway. Atypical Protein Kinase C: aPKC) phosphorylates Par-1b on a conserved threonine residue: T595) and I participated in studies demonstrating that novel Protein Kinase C: nPKC) activates Protein Kinase D: PKD) to directly phosphorylate Par-1b on serine 400: S400), a residue that …
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ANALYSIS OF TWO NOVEL COMPLEXES THAT REGULATE NEUROBLAST SELF-RENEWAL AND ASYMMETRIC DIVISION IN DROSOPHILA MELANOGASTER
… localization of atypical protein kinase C (aPKC) and cell fate determinant Numb, as well as mitotic spindle orientation in neuroblasts. The other player is Drosophila cytoplasmic dynein light chain 1, also named Cut up (Ctp). Ctp localizes at centrioles and controls the mitotic spindle …
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Opposing Crosstalk between Rho1 and Cdc42 in Epithelial Morphogenesis
… cell tension. It did so by localizing Par6/aPKC to AJs, where this complex limited Rho1 activity, and thus, acto-myosin contractility. Lastly, studies in larval imaginal discs identified Cdc42 and the Par polarity complex as novel regulators of apoptosis-induced compensatory proliferation. …
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The Nrg Growth Factors and Their Receptor ErbB4 in the Developing Brain: Delineation of Nrg3 Expression and Neuritogenesis
… members of the Par polarity complex (Par6, Par3, aPKC, and Cdc42), a group of proteins important for radial glial morphology, neuronal migration, and axonal specification. Collectively, the findings of this thesis highlight biological processes of the Nrgs and ErbB4 in the developing CNS, …
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Regulation of Cell Adhesion By The Ferm Proteins, Ptpn14 and Merlin
… the apical proteins, atypical protein kinase C (aPKC) and partitioning defective 3 (Par3), to the apical membrane for the development of functional TJs. Blocking this specific phosphorylation site disrupts the formation of functional polarized epithelia. From this, we see that distinct …
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Pluripotency and patterning in nonhuman primate embryogenesis
… deacetylase inhibition and did not require aPKC inhibition. Instead, marmoset naïve PSCs relied on exogenous ActA for long-term propagation. To functionally interrogate the signals controlling amniogenesis and gastrulation from the pluripotent EmDisc, I leveraged PSC-based embryo models. …
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Merlin Mediated Regulation of Hair Follicle Morphogenesis
… of a-catenin, the polarity proteins Par3 and aPKC as well as the tumor suppressor Merlin, the protein product of the <em>Nf2</em> gene. Merlin plays a pivotal role in the function of the JPC by bridging Par3 to a-catenin and in the absence of Merlin the interaction between Par3 and α-catenin …
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Quantitative super-resolution imaging of cell polarity proteins using DNA-PAINT
… imaging of three apical polarity proteins (aPKC, Crumbs, Par6) in the fruit fly egg chambers revealed that they form mesoscopic-sized clusters along the cell junctions. In order to analyse these clusters in a quantitative manner, I collaborated with Leila Muresan to develop an image analysis …