Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"a-Synuclein"”.
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Automated protein NMR data analysis and its application to a-synuclein fibrils
… methods to the problems of refining an a-synuclein fibril structure with proton-detected NMR data, the analysis and characterization of a pair of interrelated a-synuclein fibril strains with distinct pathological properties, and to the general question of fibril polymorphism, a phenomenon …
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Developing an a-synuclein in vitro model for therapeutic testing for Parkinson's disease
… and cerebral cortex. Genetic links between α-synuclein and PD, coupled with the identification of aggregated α-synuclein in Lewy bodies (LB), has made α-synuclein a prominent target for treating PD and related synucleinopathies. Clearing or inhibiting α-synuclein aggregation is one of the main …
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Regulation of Weibel-Palade Body Exocytosis by alpha-Synuclein in Endothelial Cells
a-Synuclein (a-syn) is a small presynaptic protein implicated in the pathogenesis of Parkinson’s disease. Although gathering evidence has been proposed for its physiological roles, the precise roles of a-synuclein and mechanisms remain incompletely understood. a-Synuclein is not only expressed in …
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Exploration of a liquid droplet state for α-synuclein in vitro and in vivo
… protein misfolding and aggregation. Misfolded a-synuclein is a major constituent of intracellular inclusions called Lewy bodies, which are a key hallmark of PD. A large body of work shows that a-synuclein can self-assemble into amyloid fibrils via a nucleation dependent pathway. However, the …
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alpha-Synuclein: Synaptische Funktion und Rolle bei der Pathogenese der Parkinson-Syndrome
Die Synuclein-Proteinfamilie besteht aus den vier Paralogen a-, b-, g-Synuclein und Synoretin. a- und b-Synuclein werden hauptsächlich im Gehirn, g-Synuclein im peripheren Nervensystem und Synoretin in der Retina exprimiert. Subzelluläre Fraktionierungen und immunzytochemische Untersuchungen …
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Quantitative analysis of proteotoxicity associated with neurodegenerative disease
… and systematically studied the toxicity of a-synuclein, which is implicated in the synucleinopathy family of diseases, including Parkinson's Disease. To systematically study the effect of toxic levels of a-synuclein expression on cellular homeostasis, I constructed a library of fluorescent …
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Modeling intrinsically disordered proteins ; a comprehensive study of [alpha]-synuclein
… substantia nigra. Several studies suggest that a-synuclein, the major component of these intracellular inclusions, plays a major role in the neurodegenerative process. Therefore understanding the structural properties of [alpha]-synuclein and its aggregation mechanism is of particular interest. …
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Implications of Advanced Glycation Endproducts in Oxidative Stress and Neurodegenerative Disorders
… antibodies to neurofilament components and a-synuclein. AGEs and a-synuclein were colocalized in very early Lewy bodies in the substantia nigra of cases with incidental Lewy body disease. These results support an AGE-induced oxidative damage due to the action of free radicals, such as NO, …
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Understanding protein misfolding diseases through the development of biophysical methods
… explored these findings in the context of a-synuclein, a protein closely associated with Parkinson’s disease. Taken together, these results illustrate the opportunities offered by combining in vitro aggregation assays with in vivo aggregation kinetics. Finally, we explored several natural …
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Anatomy of visual hallucinations and fluctuations of consciousness in Lewy body diseases
… nuclei, atrophy of neurons, neuronal death and a-synuclein deposition were assessed and relationships with clinical variables tested. These studies found changes in several thalamic nuclei, some of which are associated with disease, and others associated with particular clinical features. Visual …
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Mitochondrial protein SFXN3 as a novel target in neurodegenerative diseases
… lab has investigated proteins downstream of a-synuclein, which is a modulator of synaptic pathology, and Sideroflexin 3 (SFXN3) was identified as a mitochondrial protein that modulates synaptic morphology in vivo. This finding prompted the aims of this thesis: biochemical characterisation of …
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Order, disorder, and protein aggregation
… study, I present a conformational ensemble of a-synuclein, the culprit protein of Parkinson's disease, constructed using a Variational Bayesian Weighting algorithm in combination with NMR data collected by our collaborators. We find that the data fit a description in which the protein …
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State-of-the-art REDOR and TEDOR methods for elucidation of protein structure and interactions
… are formed due to misfolding of proteins like a-synuclein (a-syn) and amyloid-b. Understanding the structure of these amyloid fibrils will be beneficial for probing amyloid- ligand interactions, amyloid-membrane interactions and even their misfolding pathways. This dissertation thesis introduces …