Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 20 for “"Zymosan"”.
-
LPS, A TLR-4 AGONIST AND VIPER A TLR-4 INHIBITOR UPREGULATE PHAGOCYTOSIS OF ZYMOSAN IN BV2 CELLS
… (LPS) causes an upregulation in zymosan phagocytosis, a fungal cell wall extract from Saccharomyces<em> cerevisiae</em><em> </em>in microglia like BV2 cells. Furthermore, we examined the possible involvement of a β-glucan receptor on zymosan phagocytosis using laminarin, a soluble …
-
Signal transduction in macrophages. Intracellular pathways activated by microbial constituents
… LPS and peptidoglycan, the yeast preparation zymosan and ?-glucan were used to stimulate the cells. The main focus has been to elucidate the potential role of the non-receptor tyrosine kinases from the Src family (SFK) and the Tec kinase Btk. in signaling pathways induced by microbial …
-
Regulation of cytokine and chemokine production in macrophages
… of IL-10 amongst other markers. Interestingly, Zymosan stimulation of macrophages leads to a regulatory-like phenotype. Expression of SphK1 mRNA is strongly induced by Zymosan but not by LPS. Three SphK inhibitors were used to investigate the role of SphK1 in the Zymosan-stimulated macrophages. …
-
The innate response to fungal infection
… Dectin-1 specific agonists curdlan and depleted zymosan, via the ERK1/2 and p38a MAPK pathways. Further, I show that although MSKs regulate Dectin-1 induced IL-10 transcription, this does not significantly affect pro-inflammatory cytokine production. This is in direct contrast to the inhibition …
-
Modulation of Neutrophil Functions by Neurotransmitters Implicated in Stress
… NPY modulates oxidative burst (OB) triggered by zymosan in human neutrophils while it has no effects on the phagocytosis process. NPY did not trigger but did enhance OB in a time and dose-dependent fashion. The reaction was specific since non-specific peptides did not produce any significant …
-
Pathogenic effects of Mycoplasma hyopneumoniae on porcine tracheal organ cultures and neutrophils in vitro, and porcine respiratory goblet cells in vivo
… with or without M. hyopneumoniae. After zymosan stimulation, intracellular calcium levels were significantly increased in neutrophils incubated with M. hyopneumoniae. Enhancement of the zymosan-induced intracellular calcium flux suggested that M. hyopneumoniae may modulate calcium …
-
Proposed in vitro model of neutrophil swarming in a chronic, low-level inflammatory state
… Here we propose a novel model utilizing fungal zymosan coated beads as a uniform target to which neutrophils may swarm.
-
Immunostimulatory effects of different aspects of aquaculture on the host response in the edible sea urchin, paracentrotus lividus
… to several immunostimulatory agents (Chapter 4). Zymosan A was shown to be an effective immunostimulatory agent in P. lividus. Further work on handled/stored animals (Chapter 5) showed Zymosan A reduced the measured levels of some immune parameters measured relative to the control, which may …
-
Estudo comparativo do efeito de warifteína e metil-warifteína, alcaloides de Cissamplelos sympodialis ECHL (Menispermaceae), no processo inflamatório agudo.
… was assessed by intraperitoneal injection of zymosan , with consequent peritoneal lavage and cell counts by a simple optical microscope . Statistical analyzes were performed using ANOVA with Tukey post test using Graph PadPrism , ver sion 5.0 . Oral treatment with W reduced paw edema induced …
-
Characterization of the Beta-2 Adrenergic Receptor Mechanism in Bovine Neutrophils, and Some Effects of Inflammatory Stimuli on its Function
… the dose of the inflammatory stimulant opsonized zymosan eight-fold, we were able to eliminate the ability of various concentrations of terbutaline and IBMX to reduce superoxide anion production. We sought to provide a more specific demonstration of this phenomenon by activating protein kinase C …
-
Role of the Abelson Tyrosine Kinases in Regulating Macrophage Functions in Immunity and Cancer
… phagocytosis of sheep erythrocytes and zymosan particles. Treatment with the Abl kinase inhibitors imatinib and GNF-2 or overexpression of kinase-inactive forms of the Abl family kinases also impairs particle internalization in murine macrophages, indicating Abl kinase activity is …
-
The macrophage activating properties of growth hormone in vitro and in vivo: Comparison to interferon-gamma (IF N-gamma)
… of opsonized particles such as opsonized-zymosan (Op-zym) with a marked increase in the respiratory burst, NADPH oxidase system. This capacity to increase the release of reactive oxygen metabolites, including superoxide anion (O$\sb2\sp-$), plays an important role in the enhanced …
-
Identification of the Role of P-Rex1 and its Adaptor Functions in Neutrophils
… promotes the phagocytosis of IgG-opsonised zymosan particles and supports both integrin- and Fc receptor-dependent uptake independently of its Rac-GEF activity. Furthermore, P-Rex1 facilitates the Fc receptor-mediated activation of Rac and Syk. To investigate the underlying mechanism by …
-
Characterization of Pleiotropic Activities of alpha1-Antitrypsin
… lower levels of IL-8 in response to zymosan as compared to neutrophils obtained before therapy. Our results support the idea that augmentation therapy with human AAT has anti-inflammatory effects. 4. Blood monocytes from clinically healthy subjects with severe AAT-deficiency show …
-
Yersinia pestis CO92 mutant deleted for the genes encoding Braun lipoprotein and plasminogen activator protease: Characterization of a potential live-attenuated vaccine candidate
… mutant were equally efficient in their uptake of zymosan particles as determined by flow cytometric analysis. Overall, our data indicated that although the ∆lpp ∆pla double mutant of Y. pestis CO92 was highly attenuated, it retained the ability to elicit innate and subsequent acquired immune …
-
Cellular innate immune responses to lung resection via video- assisted thoracoscopic surgery (VATS) and thoracotomy : predictors of post-operative pneumonia
… reduction in neutrophil phagocytosis of zymosan (in suspension) on ANOVA modelling (p=0.047). Lung resection led to an increase in serum cytokines IL-6, IL-8 and IL-10 which peaked at 24hrs before falling (p<0.0005). ANOVA modelling confirmed significantly lower levels of serum cytokines …
-
Adrenergic Receptor Gene Expression in Bovine Leukocytes and Effects of Adrenergic Agonists on Neutrophil and Eosinophil Function
… differently to co-stimulation with opsonized zymosan and IFN and displayed significantly different responses to adrenergic agonists. Short-term treatment with E, NE, phenylephrine (an α1-AR agonist), dexmedetomidine (an α2-AR agonist), and isoproterenol (a β-AR agonist) modified neutrophil …
-
Phosphodiesterase 4 inhibitors for the treatment of inflammation associated with respiratory disease
… of inflammatory mediators [i.e. PCA, PAF and zymosan activated plasma (ZAP)]. Previous studies with PDE4 inhibitors had not examined the correlation between in vitro and in vivo activities in this way. RP73401 was the most potent PDE4 enzyme inhibitor (guinea pig macrophage) followed by SB …