Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"Zinc Fingers"”.
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A Survey of Beta-Sheet Folding: WW Domains With Zinc Fingers and Membrane Peptides
… protein. We then report on a synthetic zinc-finger peptide used to bridge the gap between experimental and theoretical protein folding research. To complete our work we summarize ongoing research on the membrane interactions of a small alpha-helical peptide.
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Design and Applications of Split-Luciferase Systems in Vitro and in Cellulo
… binding domains, specifically designed Cys₂-His₂ zinc finger domains, to split-Fluc fragments. We previously discovered that designed Cys₂-His₂ zinc fingers were not specific for targeting a variety of promoter sequences. In order to improve DNA detection, I have designed and tested split-Fluc …
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Analysis of Mouse EKLF/KLF2 E9.5 Double Knockout: Yolk Sac Morphology and Embryonic Erythroid Maturation
… family of transcription factors with 3 Cys2/His2 zinc fingers that regulate cell differentiation and developmental processes. EKLF is involved in primitive and definitive erythropoiesis; KLF2 is implicated in the development of primitive erythroid and endothelial cells of the vasculature. Using …
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The candidate tumour suppressor, XIAP associated factor 1 (XAF1), directly inhibits XIAP activity and induces G1 phase cell cycle arrest
… of 301 a.a. and contains seven potential zinc finger domains. Two alternatively splice variants of XAF1 were later isolated. One isoform (XAF1B) was formed by the removal of a 57 bp exon, which leads to an in-frame deletion of the third zinc finger and the creation of a shorter 32.5 kDa …
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Intrinsic Disorder in Transcription Factors
… because nearly a half of TFs contain several zinc-fingers and the most popular type of zinc-finger is C2H2. High occurrence of proline and glutamine in these TF datasets suggests that these residues might contribute to conformational flexibility needed during the process of binding by …
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Studies of the MYM-type zinc finger protein ZMYM3
… a transcription factor, and ZMYM3, an MYM-type zinc finger protein of unknown function. By making several truncation mutants of ZMYM3, I show that a C-terminal region containing a ‘PXP’ repeat motif mediates its interaction with RNase H2, and that the PXP-containing proteins ZMYM2 and ZMYM4 …
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THE ROLE OF TIGHT JUNCTION PROTEIN ZO-2 IN THE NUCLEUS AND ITS LINK TO TRANSCRIPTION FACTORS OF THE SLUG/SNAIL FAMILY
… localization signals, nuclear export signal and zinc fingers that are important for DNA binding and nuclear retention. Furthermore, ZO-2 may also influence the sub-cellular localization of this transcription factor, for example in wound healing assays. Wounding of the cell monolayer raises the …
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The development of a model system and high throughput assay for the study of interactions between zinc finger proteins and DNA
… which utilise small DNA binding motifs called zinc fingers, are presently employed. None of the current approaches successfully combine a combinatorial approach to the elucidation of a code with a single stage high throughput screening assay. The work outlined here describes the development of …
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Recombinant Expression, Purification, and Characterization of an HIV-1 Tat-Human Cyclin T1 Chimera
… critical to the formation of the second of two zinc fingers (Zn2). Here we suggest that it is the presence of this critical cysteine residue and not the presence of arginine residues from human Cyclin T1 that imparts high affinity and specificity to the interaction with HIV-1 TAR RNA. Crucial …
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Applying engineering principles to the design and construction of transcriptional devices
… a synthetic transcription factor derived from a zinc finger DNA binding domain and a leucine zipper dimerization domain. Zinc fingers and leucine zippers offer a potential scalable solution to the challenge of building libraries of transcription-based logic devices for arbitrary information …
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Molecular Mechanisms of C11orf95 translocations in cancer.
… transcriptional activators. We find that the Zinc Fingers (ZF) of C11orf95 promote nuclear translocation and a common unique transcriptional signature mediated by the trans-activation domains (TAD) of the fused c-terminal protein partner. Specifically, the ZF’s of C11orf95 direct DNA-binding …
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Farnesylation-dependent regulation of transcripts by PARP13
… extent to RNA interactions through its CCCH zinc fingers. We also identify a set of putative PARP13.1 cellular mRNA targets by differential expression analysis of PARP13-/- cells expressing either full length PARP13.1, a RNA binding mutant (PARP1 3 .1VYFHR) or a farnesylation mutant …
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Bio-Nano Interfaces: Enzyme Immobilization for Biomimetic Energy Harvesting
… by small protein structural motifs, called zinc fingers (ZnF) that bind to specific dsDNA sequences and may be genetically bound to small laccase (SLAC-3ZnF) or other redox enzymes. Utilizing scaffolds with gold nanoclusters (AuNC) for mediated electron transfer (MET), we test the …
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Unraveling the interactions between MBNL1 protein and its RNA targets
… is an RNA binding protein with four conserved zinc fingers that plays an important role in the regulation of alterative splicing. It interacts with pre-mRNA and promotes the inclusion or exclusion of exons to form different mRNA transcripts. Besides its regulatory function in alternative …
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Function and regulation of PARP13 binding to cellular RNA
… binding viral transcripts via its four CCCH-type zinc fingers and targeting them for degradation. In the context of the immune response PARP1 3 can also inhibit the translation of its viral targets and enhance the activity of other RNA-binding viral receptors, such as RIG-1. More recently PARP13 …
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Site-Specific Genome Engineering in Mouse Primary Fibroblasts
Site-specific genome engineering is a powerful tool for medical therapeutics and basic scientific research. As the name implies, site-specific genome engineering describes the ability to add or subtract nucleic acid information in a precise, controlled manner within the genome. This technology has …
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Site-Specific Genome Engineering in Mouse Primary Fibroblasts
Site-specific genome engineering is a powerful tool for medical therapeutics and basic scientific research. As the name implies, site-specific genome engineering describes the ability to add or subtract nucleic acid information in a precise, controlled manner within the genome. This technology has …
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Caracterización funcional de Snail2 como represor de cadherina- E y como regulador del proceso de carcinogenésis química
… which in turn induce TEM, including factors the zinc finger family of Snail (Snail1 and Snail2) and ZEB (ZEB - 1 and ZEB-2) and factors of the bHLH family (E47 and Twist). Previous studies of our laboratory, comparing gene expression profiles analysis of MDCK-II cells with stable expression of …
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The role of Wilms’ Tumor gene product (WT1)in CD95-mediated apoptosis in T-cell leukaemias
… it. Wilms’ tumor gene 1 (WT1) encodes a four-zinc-finger protein. Alternative splicing at the pre-mRNA level yields several transcript variants, of which the best characterized are the so called WT1-KTS and WT1+KTS isoforms. The WT1-KTS isoform, which lacks three amino acids between …
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Recombinant expression, purification and structural characterization of Saccharomyces cerevisiae RING finger domain using Nuclear Magnetic Resonance (NMR) spectroscopy
… of cysteine sequence motif that binds to two zinc atoms. It has a cross-brace topology consisting of 70 amino acids and its sequence is defined as Cys1-Xaa2-Cys2-Xaa9–39-Cys3-Xaa1–3-His4-Xaa2-3-Cys/His5-Xaa2-Cys6-Xaa4–48-Cys7-Xaa2-Cys8 (where Xaa represents any amino acid residue). The RING …
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