Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"ZGA"”.
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The roles of DPPA2, DPPA4 and SMARCA5 in mouse zygotic genome activation, epigenetic reprogramming and development
… a process also called zygotic genome activation (ZGA). In mice, this takes place in 1 cell embryos – minor ZGA, followed by a burst of transcription - major ZGA, at the 2 cell stage. Previous screens in the lab, using mouse embryonic stem cell (mESC) identified the small DNA binding proteins …
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CRISPR activation screening at single-cell transcriptomic resolution: discovering new regulators of zygotic genome activation
… zygotic genome. Zygotic genome activation, or ZGA, coincides with dramatic epigenetic reprogramming processes and is likely orchestrated by the interplay of epigenetic and transcriptional regulators. In this dissertation, I develop a novel high-throughput screening method that combines pooled …
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The role of inherited determinants and embryonic-extraembryonic cell interactions in models of mammalian embryogenesis
… the zygotic genome. Zygotic genome activation (ZGA) is critical for successful embryogenesis, and yet the precise mechanisms of its regulation remain elusive. The first aim of this thesis was to assess the contribution of inherited cell cycle regulators to the onset of ZGA in the early mouse …
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MODELING INFERTILITY: SINGLE GENE MUTATIONS AND CONSEQUENCES FOR GERM CELL DEVELOPMENT
… I in mice and for zygotic genome activation (ZGA) in Ciona intestinalis. In humans, CCNB3 mutations cause recurrent pregnancy loss. We explored meiotic and post-meiotic roles of CCNB3 using imaging studies, fertility tests, and pronuclei swap experiments. Ccnb3-/- females are sterile. Ccnb3-/- …
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Role Of Maternal Sin3a In Reprogramming Gene Expression During Mouse Preimplantation Development
… maternal SIN3A does not play a role in the minor ZGA, as depleting maternal SIN3A had no effect on global transcription in 1-cell embryos, but surprisingly results in histone hypoacetylation in 1-cell mouse embryos. Maintaining the presence of maternal SIN3A beyond the 1-cell stage had no effect …
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Characterization of Maternal Factor MOF/H4K16ac and the Impact of the Cryostress on Bovine Embryonic Development
Precise gene regulation is critical for mammalian embryo viability. Bovine embryos, which share high transcriptomic similarity with humans, serve as valuable models for understanding fundamental biological processes. Insights gained from studying bovine embryogenesis can transduce to broader …