Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"Yeast-Two-Hybrid-Screening"”.
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Identification of Artemis and PARP-1 interacting factors: A yeast two hybrid screening approach
… repair) or apoptosis. Artemis and PARP-1 are two proteins with significant implications in the various branches within the DNA damage response network. A series of yeast two hybrid screening (Y2H) experiments were conducted using both classical and interacting mating Y2H systems to find …
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Suche nach Interaktionspartnern mit dem ATP-abhängigen Kaliumkanal der Niere, ROMK, durch "Yeast-Two-Hybrid-Screening"
… Proteinen erhoffen. Nach Durch-führung dreier „Yeast-Two-Hybrid“-Screens zur Identifizierung möglicher Interakt-ionspartner von ROMK kamen 17, von ihrer Funktion schon bekannte, aussichtsreiche Proteine, in die enge Auswahl. Nach weiterer Charakterisierung und Autoaktivierungs-tests blieben 13 …
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Identifizierung neuer Interaktionsproteine des humanen Septins SEPT4 (hCDCrel-2) in fetalem Gehirn
… von SEPT4 in fetalem Gehirn mit dem Yeast Two-Hybrid System charakterisiert: SEPT8, SEPT11 und die Proteine Filamin B, MURR1 und die Ubiquitin-spezifische Protease 31. Es wurde in dieser Arbeit gezeigt, dass auch der N-Terminus von SEPT4 in Interaktionen mit Septinen und anderen …
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Characterization Of A Novel Interactor/substrate For The Pro-apoptotic Serine Protease Omi/htra2
… p38 stress-activated kinase, using a modified yeast two-hybrid system. Omi localizes in the mitochondria and in response to apoptotic stimuli the mature form of this protein translocates to the cytoplasm. In the cytoplasm Omi participates in both the caspase-dependent as well as …
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Biological studies of organellar (Na⁺,K⁺)/H⁺ exchanger NHE7
… of the novel class of Na⁺/H⁺ exchanger NHE7, yeast two-hybrid screening was conducted and several binding candidates were identified. Among these candidates, I show that Secretory Carrier Membrane Proteins (SCAMPs) are novel NHE7 binding proteins and that SCAMPs regulate endocytic trafficking …
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Role of the Sh3 and Cysteine-Rich Domain 3 (STAC3) Gene in Proliferation and Differentiation of Bovine Satellite Cells
… gene predicted to encode a protein containing two SH3 domains and one cysteine-rich domain. In this study, we determined the potential role of the STAC3 gene in proliferation and differentiation of bovine satellite cells. We isolated satellite cells from skeletal muscle of adult cattle and …
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Effects of DFO-Induced Hypoxia on Key Signaling Mediators
… negatively regulate MOR gene expression using a yeast two-hybrid screening system. RT-PCR analysis revealed an increased in RACK-1 expression while a decrease in MOR expression under DFO treatment was observed. These results supported our recent finding that RACK-1 participates in the regulation …
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STRUCTURE-FUNCTION STUDIES OF TRANSLOCATED EFFECTORS FROM THE LATE BLIGHT PATHOGEN
… This work details structure-function studies of two translocated effectors from the late blight pathogen; PexRD2, a representative RXLR effector, and CRN8, an effector with kinase activity. Efforts to heterologously express CRN8 were hampered by protein insolubility and low yields. However, the …
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Molecular Mechanisms Underlying Functions of Juvenile Hormone Receptor
… formed with other proteins of this family. In a yeast two-hybrid screening, we discovered that another bHLH-PAS family protein, FISC, interacts with Met in the presence of JH. FISC is also required for JH functions as an RNAi knockdown of FISC down-regulated JH-responsive genes. To elucidate how …
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Identification of Substrates and Pathways Regulated by WNK1
… by the discovery that mutations in at least two of the four human WNKs, WNK1 and 4, caused a heritable form of hypertension. My study focused on unveiling WNK1 substrates and interactors for a better understanding of the molecular pathways served by WNK kinases. Yeast two-hybrid screening was …
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The role of DAXX in the selective autophagy receptor SQSTM1/p62 phase condensation
… to identify a mechanism of it. Via an unbiased yeast two-hybrid screening, DAXX is found a p62 binding protein. Further experiments confirmed that cytoplasmic DAXX promotes p62 puncta formation and drives p62 liquid phase condensation. In vitro experiments also confirmed that DAXX promotes p62 …
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Engineering Estrogen Receptor-Based Gene Switches and a Superoxide Dismutase for Therapeutic Applications
… This dissertation describes the application of a hybrid directed evolution/rational design approach for the engineering of two different proteins with desirable properties. In the first example, stepwise saturation mutagenesis of functionally important protein sites and random point mutagenesis …
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Neurologin Function in Excitatory and Inhibitory Synapses
… of synaptic density by NL1 full length. By yeast two hybrid screening using NL2 cytoplasmic region, we identified potential interaction partners, of which Necab2 and NRP/B (also named as ectodermal cortex 1, EC1) are two promising candidates and the interactions were confirmed. NL1 or NL2 …
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Understanding HNF-1β through Identification of Interacting Proteins and Target Genes in the Kidney
… the function of HNF-1Beta in the kidney. Yeast two-hybrid screening was performed to identify binding partners of HNF-1Beta in the kidney. Zyxin and LPP were isolated as putative interacting proteins. The LIM-containing proteins, Zyxin and LPP, are focal adhesion proteins that shuttle …
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Characterization of intracellular interactions between dengue virus and host proteins
… fever and dengue shock syndrome. About two-fifths of world population live in areas where dengue is prevalent, leading to high levels of morbidity and mortality in many areas. Currently there are no vaccines or effective treatments. The virus is transmitted from one person to another by …
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Regulation der humanen Aurora-A Kinase und Identifikation potentieller Interaktionspartner
… from cell extracts. In addition Yeast-Two-Hybrid Screening and fishing experiments with recombinant proteins were performed. These studies provided four new putative Aurora-A interacting partners (KIAA1007, KIAA1741, TPX2 and a candidate from the Yeast-Two-Hybrid Screen). The …
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Viral subversion of host cell membrane trafficking
… between host cell factors and proteins of two different enveloped viruses: hepatitis D virus (HDV) and herpes simplex virus 1 (HSV-1). HDV is a small RNA virus causing fulminant hepatitis or severely aggravating cirrhosis and hepatocellular carcinoma. HSV-1 is a large DNA virus infecting …
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Zur Expression und Funktion von Prm3: ein ungewöhnliches Protamin
… der Protamin-Familie. Desweiteren wurde im Yeast-Two-Hybrid- Screening nach putativen Interaktionspartnern von Prm3 gesucht. Von den ursprünglich 6 isolierten Kandidatenklonen zeigte lediglich ein Klon (ein bislang unbekannter EST-Klon) auch im Mammalian-Two-Hybrid-System eine spezifische …
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Transcription Factor Engineering: Tools and Applications
… the estrogen receptor. Beginning with two ligand binding domain mutants previously engineered to recognize the small molecules 4,4’-dihydroxybenzil (DHB) or 2,4-di(4-hydroxyphenyl)-5-ethylthiazole (L9), I showed that they could be used as a gene switch to independently control reporter …
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