Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"Yeast-2-Hybrid"”.
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A yeast 2-hybrid screen to identify and characterize interaction partners of the cancer associated protein retinoblastoma binding protein 6
… novel protein interacting partners using a yeast 2-hybrid screen.In order to identify interaction partners of RBBP6, two well characterized domains of RBBP6, the N-terminal ubiquitin-like DWNN domain and RING finger domain, were used as baits in a yeast 2-hybrid screen of a human testis cDNA …
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Dual Roles for NBR1 in Bone Remodelling and Autophagosomal Protein Degradation
… Nbrl by identifying novel protein interactors. A yeast-2-hybrid screen revealed a direct interaction between Nbrl and the autophagic effector protein LC3. This suggested a role for Nbrl in the autophagic degradation of polyubiquitinated proteins. A further yeast-2-hybrid screen identified a direct …
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Structural Characterization of the Flavonoid Enzyme Complex
… docking, molecular dynamics simulations, and yeast 2-hybrid analyses. These enzymes appear to be interacting in a manner that could facilitate the flow of intermediates from one active site to another. These experiments also identified a series of amino acids that appear to be involved in the …
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Structure/Function Studies on Vps9 domain-containing Ankyrin Repeat protein (VARP)
… Varp (residues 1-136) was investigated using a Yeast-2-Hybrid screening approach, to try to identify any interaction partners that bind in this region. Many potential interaction partners were discovered, however neither Kif5a nor golginA4 was detected binding in this region.
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DISCOVERY AND FUNCTIONAL ANALYSES OF HESSIAN FLY EFFECTOR-ENCODING GENES
… F-box and leucine rich repeats (LRR) domains. Yeast 2-hybrid experiments showed that SSGP71 members interact with host plant Skp1-like proteins. In chapter four, I use bacterial T3SS-based delivery of proteins into plants to test candidate HF effector-encoding genes. Candidate vH13 failed to …
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Investigating the role of Klhl31 in striated muscle
… to C2C12 differentiation. Furthermore, we used a Yeast-2-Hybrid screen approach and GST-pull downs to find interaction partners for Klhl31. Putative interacting proteins for Klhl31 were analysed and found to be structural components of the sarcomere with many of them also being involved in …
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The role of ACK in regulating the Vav family proteins
… cancer therapy, the Owen/Mott group performed a yeast-2-hybrid screen to identify new binding partners of ACK. 14 novel proteins that interact with ACK were identified, one of which was a small G protein regulator Vav3, the least-studied member of Vav-family proteins. The first part of this …
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MED9, a Mediator Complex Component, and its Interacting Protein MORC1 Balance Growth and Defense in Arabidopsis
… 14 MORC1-interacting proteins (MIPs) via yeast 2-hybrid screening, eight of which have putative nuclear-associated functions. While a few loss-of-function mutants for these MIPs displayed altered bacterial resistance in Arabidopsis, MIP13 was highly unusual. MIP13’s single mutant was …
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STK25 and TAO Regulate Muscle-Specific Transcription and Muscle Function in the Parasitic Flatworm Schistosoma Mansoni
… STK25 kinases in other organisms. Using a yeast 2-hybrid system, we find that schistosome STK25 and CCM3 can physically interact, in agreement with literature reporting this interaction in other organisms. Altogether, our data supports a model that these genes work together in the parasite …
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Molecular Mechanism of Aurora-A Kinase in Human Oncogenesis
… which may contribute to human oncogenesis. Using yeast 2-hybrid screening, we identified a splicing form of CNTD2 as Aurora-A interacting protein. CNTD2 locates to chromosome 19q13.2 AKT2 amplicon. CNTD2 is amplified and overexpressed in human ovarian, pancreatic and lung cancer cell lines and …
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The analysis of Autographa Californica multiple Nucleopolyhedrovirus EXONO (ORF141) function and its role in virus budding
… fractions of both BV and ODV. In support of this yeast 2-hybrid screening, co-immunoprecipitation, and confocal microscopy revealed that EXON0 interacted with the known nucleocapsid proteins FP25 and BV/ODV-C42. Transmission electron microscopy showed that deletion of exon0 results in …
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The involvement of ARF6 in rapid membrane recycling during Drosophila spermatocyte cytokinesis
… Pavarotti, identified as an ARF6 interactor in a Yeast-2-Hybrid assay, binds ARF6 in GST pulldowns, and interacts genetically with the arf6 mutant. ARF6 localizes to the plasma membrane and a population of early and recycling endosomes. During cytokinesis, ARF6 is enriched on recycling endosomes …
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Functional Analysis of Plant Glutamate Receptors
… subunits to form functional channels. A modified yeast-2-hybrid system approach was initially taken and applied to 14 of the 20 AtGLRs to identify a number of candidate interactors in yeast. Forster resonance energy transfer (FRET), which measures the transfer of energy between interacting …
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Examination of 2-Oxoglutarate Dependant Dioxygenases Leading to the Production of Flavonols in <i>Arabidopsis thaliana</i>
… roles in flavonol production were explored by yeast 2-hybrid analysis.
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A putative interaction between mitochondrial cytochrome c oxidase subunit II (COX II) to lamin A/C and LAP2α in colon epithelial cells.
… A/C and LAP2α in colon epithelial development,a Yeast 2-hybrid screen was used to look for novel protein interactions to Lamin A/C and/or LAP2a. It was found Cytochrome c oxidase subunit II (Cox2) is a putative binding partner to Lamin A/C and LAP2 α. Cox2 is encoded by the mitochondrial genome …
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Characterising GIGANTEA interactors: the BELL-LIKE HOMEODOMAIN 3 and BELL-LIKE HOMEODOMAIN 10 proteins
… protein interactor. As part of this thesis work, yeast 2-hybrid and in vitro pull down assays were utilised to confirm the interaction between GI and BLH3. Sequence and phylogenetic analyses were used to further examine the BELL family of proteins. The BELL-LIKE HOMEODOMAIN 10 (BLH10) protein was …
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Investigating the Functional Interaction between RhoGDI Family Proteins and Activated Cdc42 associated-kinase (ACK)
… normal and pathological conditions. A previous yeast-2-hybrid screen in the lab identified RhoGDI-3 as an interacting partner for ACK. RhoGDI-3 is a member of RhoGDI family of proteins, which are negative regulators that maintains Rho-family GTPases in the inactive GDP-bound state and sequesters …
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Algorithms for the analysis of protein interaction networks
… in a popular experimental protocol, the Yeast 2-Hybrid method. Here, some "promiscuous" proteins may lead to many false positives. We describe a Bayesian approach to modeling and adjusting for this error. 3. Comparative analysis of PPI networks across species can provide valuable …
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Characterising the role of GPR50 in neurodevelopment and lipid metabolism
… with altered lipid levels in humans. Further, a yeast-2-hybrid screen identified SREBF2 and ABCA2, regulators of lipid homeostasis, as GPR50 interactors. This thesis explores the role of GPR50 in neuronal development and lipid metabolism. The work presented in this thesis shows that GPR50 …
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Identification of host proteins targeted by the flax rust effector protein AvrL567
… To identify targets of the effector proteins, Yeast-2-Hybrid (Y2H) screens were performed with cDNA from rust infected flax leaves. This approach allowed for the identification of plant targets as well as potential fungal cofactors. Eight putative interactors (iA1 to iA8) were isolated for …
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