Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"Yeast surface display"”.
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DESIGNING A PLATFORM FOR DEVELOPING NOVEL GLYCAN-BINDING PROTEINS USING YEAST SURFACE DISPLAY
… of alternative protein scaffold libraries using yeast surface display. A library of the tenth repeat, type III domain of human fibronectin (FN3) was screened against polysialic acid, a glycan found in certain cancers, and enriched using a combination of random mutagenesis, magnetic-activated cell …
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Affinity Maturation of the D1.3 Antibody Using Yeast Surface Display and Flow Cytometry
… affinities attained previously with phage display for anti-protein antibodies, without the necessity for large libraries or site-directed saturation mutagenesis.
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Mechanistic studies of class II lanthipeptide synthetases and yeast surface display of lanthipeptide leader peptides
… of a LanM with the substrate bound to it. Yeast surface display was selected to engineer the LctA leader for better binding affinity to the LctM enzyme. Two rounds of sorting resulted in several mutations in the leader region, including three consistent aspartate mutations. Using …
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Engineering of HIV gp120 by yeast surface display for neutralizing antibody characterization and immunogen design
… impossible. In this thesis, we establish a yeast surface display system for the development of gp120-based molecules for antibody characterization and immunogen design. A stripped core gp120 is constructed that retains the correct presentation of the CD4 binding site. Epitopes of several CD4 …
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Engineering and Characterization of a Single Chain Antibody Specific for Murine CD28
… stability of engineered antibody fragments using yeast surface display and flow cytometry was produced. This method allowed for the rapid determination of relative temperature stability without the need to produce large quantities of protein. This method compared favorably to other methods relying …
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Towards rational design of peptides for selective interaction with inorganic materials
… study of the peptide-inorganic interface using Yeast Surface Display. The general approach is to use combinatorial selection to suggest interaction principles followed by rational design to refine understanding. In this pursuit, two material groups-II-VI semiconductors and synthetic sapphire …
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Molecular Engineering of a Single-Chain FV Antibody Fragment to Femtomolar Affinity
… have been isolated from mutagenized libraries displayed on yeast and screened by flow cytometry. Analysis of the nature of mutations conferring binding improvements suggests a potentially general strategy for engineering scFv antibodies for high affinity binding to target ligands. The results …
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Characterizing and engineering antibodies against the epidermal growth factor receptor
… library of random mutants of the EGFR antigen is displayed on the surface of yeast, and the library is combinatorially selected for loss of binding to the antibody being mapped. If a mutant shows loss of binding to an antibody, then that residue is a potential contact residue. In addition, we …
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Identification and Knockout of Immunodominant Endogenous Retroviral Antigen in Murine Tumor Models
… (env) of eMLV was found to be the dominant cell-surface antigen targeted by iiAbs. Based on this finding, we studied the role of anti-env antibodies in an anti-tumor response. An anti-env antibody termed 1E4 was isolated from a cured mouse by single-cell cloning, and its affinity was improved by …
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The Production of Recombinant Immunoglobulin Superfamily Proteins in the Yeast Saccharomyces Cerevisiae
… improved using a directed evolution approach and yeast surface display. Evolution resulted in a super-scTCR scaffold with optimized secretion, solubility, and stability properties. This scaffold will, for the first time, enable directed evolution of the binding properties of the scTCR for its …
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Increasing Heterologous Protein Secretion From the Yeast S. Cerevisiae Through Manipulation of Cellular Redox Factors
… A system has been set up to utilize the yeast surface display system to screen a cDNA library for factors that increase BPTI surface display, and therefore secretion. The information gathered in this work identifies new directions for increasing protein secretion in yeast.
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A directed evolution approach to engineering recombinant protein production in S. cerevisiae
… in secretory selection. In this thesis, yeast surface display is investigated as a potential proxy for heterologous protein secretion. Although ultimately unsuitable as a screening proxy, the surface display experiments did show a novel method of improving protein secretion by …
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Molecular recognition of chlorine-doped polypyrrole
… bulk properties. Through the use of both phage display of peptide libraries and yeast surface display of scFv libraries the surface of chlorine-doped polypyrrole (PPyCl) has been modified to facilitate binding of neuronal phenotype cells. The selection of peptides using phage display found a …
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Isolation, engineering, and characterization of intracellular antibodies specific for the huntingtin protein
… reductions in htt aggregation. We have used yeast surface display (YSD) of antibodies combined with fluorescence activated cell sorting (FACS) to isolate novel single-chain antibody (scFv) clones against huntingtin from a non-immune human antibody library; these scFv's did not inhibit htt …
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Applications of yeast display in synthetic biology with a focus on biological synthesis and recycling of plastics
Yeast surface display has emerged as a versatile tool in synthetic biology, particularly in protein engineering. This work sought to improve the flexibility and ease-of-use of yeast display for synthetic biologists, as well as applying the yeast display system in several contexts – with a …
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Discovering and Characterizing New Homing Endonucleases for Genome Engineering
… ~20 bp. In many eukaryotes, including humans and yeast, double-strand breaks induced by LHEs stimulate repair by Homologous Recombination, which can be used to alter or repair a gene if the template is supplied in trans, and Non-Homologous End Joining, which can be used to knock out a gene. The …
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Isolation and engineering of a high affinity antibody against P-selectin glycoprotein ligand-1 (PSGL-1)
… and in vivo homologous recombination to mimic in yeast the broad spectrum of mutagenic strategies exploited by B cells such as somatic hypermutation, receptor revision (... CDR replacement), receptor editing (chain shuffling), and amino acid insertions and deletions. Together with yeast surface …
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Approaches towards development of novel fluorogenic biosensors for detection of small protein analytes
… native ErbB-ligands are reported. Using yeast surface display, two libraries of protein-binding scaffolds were engineered to bind selectively to human EGF and human betacellulin with low nM KD. Bio-orthogonal conjugation of these protein scaffolds to 4-DMN, a solvatochromic fluorophore, …
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Engineered biomolecular interactions with inorganic materials : sequence, binding, and assembly
… principles governing peptide-inorganic material surface interactions at the level of amino acid functional groups. To facilitate fundamental studies, methodologies broadly applicable to probing sequence-activity relationships of peptide-inorganic material surface interactions were developed. A …
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Antibody engineering for tumor immunotherapy
… antigen. CEA is also quite stable, with a cell-surface shedding half-life of approximately 7 days. Directed evolution methodology has been utilized to design an antibody fragment with properties that would improve tumor retention. Specifically, antibody engineering methods were used to produce a …
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