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Showing 1 to 20 of 41 for “"Yeast Two-Hybrid system"”.

  1. Development of a split-ubiquitin based, cytosolic yeast two-hybrid system and its application to the structure-function analysis of scUri1p

    Möckli, NatalieETH2007

    ethz Repository record

  2. Interaction studies of the cellular prion protein

    Hundt, ChristophLudwig-Maximilians-Universität

    … under native conditions. This was proven in the yeast two-hybrid system followed by the identification of two interaction domains. The influence of mutations and polymorphisms within the prion gene was investigated in the yeast two-hybrid system. This method is also an useful tool for the …

    lmu-germany Repository record

  3. Das Sap47-Gen aus Drosophila melanogaster : Gezielte Mutagenisierung und Suche nach Interaktionspartnern

    Funk, NataljaUniversität Würzburg

    … das SAP47 Protein zu identifizieren wurden ein Yeast-Two-Hybrid System und das "CytoTrap" Verfahren eingesetzt.

    wurz-thes Repository record

  4. Molekulare Analyse der Yersinien-Wirtszell Interaktion

    Abahji, Thomas N.Ludwig-Maximilians-Universität

    … welches für ein Typ III Sekretion/Translokationsssystem und einige Virulenzfaktoren, sogenannte Yops (Yersinia outer proteins) kodiert. Die sechs bisher bekannten Effektor-Yops gelangen über das Sekretionssystem ins Zytoplasma von Makrophagen und Granulozyten, was in diesen zu einer Inhibition von …

    lmu-germany Repository record

  5. Structural and Functional Characterization of GAPR-1, a Mammalian Plant Pathogenesis-related Protein in Lipid-enriched Microdomains of the Golgi Complex

    Serrano, Ramon LeonardoUniversität Heidelberg

    … confirmed by crosslink experiments and by the yeast two hybrid system. By affinity chromatography, GAPR-1 was shown to interact with three proteins: Nucleolin, Template activating factor α (TAFIα) and HSAPRIL. In the yeast two hybrid system, the interaction of GARP-1 with Nucleolin was …

    heid-diss Repository record

  6. Identifizierung neuer Interaktionsproteine des humanen Septins SEPT4 (hCDCrel-2) in fetalem Gehirn

    Horn, Julia Catherina

    … von SEPT4 in fetalem Gehirn mit dem Yeast Two-Hybrid System charakterisiert: SEPT8, SEPT11 und die Proteine Filamin B, MURR1 und die Ubiquitin-spezifische Protease 31. Es wurde in dieser Arbeit gezeigt, dass auch der N-Terminus von SEPT4 in Interaktionen mit Septinen und anderen …

    freiburg-diss Repository record

  7. Identifizierung und Charakterisierung von Interaktionen der Nichtstrukturproteine NS1 und NS2 des Respiratorischen Synzytialvirus mit Proteinen der Wirtszelle

    Wolff, MichaelLudwig-Maximilians-Universität

    … like Respiratory Syncytial Virus (RSV) encode two nonstructural proteins, NS1 and NS2. These two proteins cooperatively mediate type I interferon resistance and prevent induction of interferon in infected cells. Interactions of NS1 and NS2 proteins in every combination were shown by using the …

    lmu-germany Repository record

  8. Yeast Dynamin Functions with Clathrin at the Golgi

    Williams, Michelle Amber

    … protein sorting) is a GTPase protein and a yeast ortholog to the mammalian dynamin-like protein (Dlp1). Vps1 acts cooperatively with many proteins at diverse cellular locations for endocytosis, protein sorting, and membrane fusion and fission. Classical dynamin family proteins work in …

    mo-state Repository record

  9. Novel Physical Interactions of Vps1 with Major Intracellular Traffic Regulators

    McDermott, Hyoeun Ahn

    Vacuolar Protein Sorting 1 (Vps1), the yeast homolog to human dynamin, is a GTPase that acts as a pivotal component in membrane trafficking. In light of observations that VPS1 displayed positive genetic interactions with four genes implicated in the recycling traffic (YPT6, GYP1, RCY1, and RIC1) …

    mo-state Repository record

  10. Identification of binding protein with kinesin18B

    김, 혜진2011

    … a protein that interacts with kinesin18B using a yeast two-hybrid system, GST pull-down assay, immunoprecipitation. This protein is Microtubule-associated protein RP/EB family member 1 containing a calponin homology(CH) domain at their N terminus and an EB1-like C-terminal motif at their C …

    ajou Repository record

  11. Identification and characterization of Risp

    Ceccherini-Silberstein, FrancescaLudwig-Maximilians-Universität

    … search for new Rev-interacting factors using the yeast two-hybrid system. In this work a new Rev-interacting protein has been identified, by screening a Jurkat T cell cDNA library. The protein was termed Risp (Rev-interacting shuttle protein), because it shuttles between the nuclear and the …

    lmu-germany Repository record

  12. Yeast two-hybrid analysis of skeletal muscle triad junction proteins

    Proenza, Catherine, author, Beam, Kurt G., advisorColorado State University. Libraries1999

    … triad junctions. In this dissertation, the yeast two-hybrid system was used to search for interactions among known and novel proteins of triad junctions. In direct interaction tests, no strong interaction was detected between the dihydropyridine receptor (DHPR) II-III loop and the skeletal …

    colostate Repository record

  13. Characterization Of A Novel Interactor/substrate For The Pro-apoptotic Serine Protease Omi/htra2

    Stratico, Valerie Anne

    … p38 stress-activated kinase, using a modified yeast two-hybrid system. Omi localizes in the mitochondria and in response to apoptotic stimuli the mature form of this protein translocates to the cytoplasm. In the cytoplasm Omi participates in both the caspase-dependent as well as …

    ucf

  14. Characterization of ESl47, a salt stress regulated protein kinase from Lophopyrum elongatum

    Elias, Demetra2001

    … in salt tolerance and salt stress response. The yeast two-hybrid system was used to screen a cDNA Arabidopsis library and identify proteins that interact with the ESI47 kinase. Six partial DNA sequences encoding a 33 kDa oxygen-evolving enhancer precursor protein (OEE1) from the photosystem II …

    concordia Repository record

  15. Die Funktion des spaghetti-Gens in Drosophila melanogaster

    Marhold, JoachimUniversität Heidelberg

    … interagiert mit dem Spaghetti-Protein sowohl im yeast two-hybrid System als auch im GST-'binding' System TPR-Domänen abhängig. Ektopische Expression des Spaghetti-Proteins führt zu Formationen von Extravenen in den Flügeln der Fliege sowie zu abnormaler Differenzierung der scutellaren bristless. …

    heid-diss Repository record

  16. Identification of proteins interacting with lupin and Arabidopsis tRNA nucleotidyltransferases

    Gu, Jun2000

    … or Arabidopsis tRNA nucleotidyltransferase, the yeast two-hybrid system was employed to screen an Arabidopsis cDNA library. As a result, 11 cDNA clones have been isolated belonging to four different classes of cDNAs. Representatives from each class were sequenced in their entirety. Sequence …

    concordia Repository record

  17. Archaeal Basal Transcription Factors: DNA and Protein Interactions

    Colon Gonzalez, Gretchen MarieUniversity of Illinois at Urbana-Champaign2015

    The archaeal basal transcription system is a stripped-down model of the complex eucaryal one, having only one RNA polymerase and two known and characterized basal factors: Transcription Factor B (TFB) and TATA-Binding Protein (TBP). After the first sequence analysis of an archaeal genome (Bult et …

    uiuc Repository record

  18. Interactions between Nova and brPTB Proteins in Alternative Splicing and Nuclear Localization

    Polydorides, Alexandros Demetrios

    … of alternative splicing. In this study, the yeast two-hybrid system is used to isolate Nova-1nteracting proteins. A novel RNA-binding protein named brPTB is identified, that is closely related to the polypyrimidine tract-binding protein (PTB) and is ennched in the brain at the mRNA and …

    rockefeller Repository record

  19. Possible Interacting Proteins for Retinol Binding Protein and Cellular Retinol Binding Protein

    Cooney, DavidNational University of Ireland Maynooth2011

    … inside the cell. It travels in the circulatory system attached to Retinol Binding Protein (RBP) and its transport into the cell is mediated by the RBP receptor STRA6. Inside the cell, retinol binds to cellular Retinol Binding Protein (CRBP) and can be converted to retinoic acid, which can then …

    maynooth Repository record

  20. Identification of proteins interacting with the human mismatch repair protein MLH1

    Mac Partlin, MaryUniversity of Glasgow2000

    … of downstream damage response pathways. A yeast two-hybrid system, an in vivo system for detecting protein-protein interactions was utilised for this purpose. Fifteen known and five unknown genes were identified as encoding proteins interacting with hMLH1. These included three known hMLH1 …

    glasgow Repository record

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