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Showing 1 to 20 of 37 for “"Yarrowia Lipolytica"”.
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Investigations of lipid metabolism in Yarrowia lipolytica
… of the lipid metabolism pathway in the yeast Yarrowia lipolytica was conducted. Yarrowia is an oleaginous ascomycete that is capable of growing on many different substrates, which derives its name from its high efficiency of growth on lipids. Once the exogenous lipids are converted into free …
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Molekulare Charakterisierung des Retrotransposons Ylt1 der Hefe Yarrowia lipolytica
… des eukaryotischen Genoms. In der Hefe Yarrowia lipolytica existiert neben anderen Retrotransposonen das Retrotransposon Ylt1 (Yarrowia lipolytica retrotransposon). Dieses Retrotransposon besteht aus den flankierenden "Long Terminal Repeat" (LTR), zeta von jeweils 714 …
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Autophagic degradation of peroxisomes in the alkane-assimilating yeast Yarrowia lipolytica
… of peroxisomes (pexophagy) in the yeast Yarrowia lipolytica. This microorganism has been extensively used to explore peroxisome biogenesis (Titorenko and Rachubinski, 2000). Gunkel et al. (1999) intoduced Y. lypolitica into pexophagy studies. However, the field of pexophagic research on …
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Μεταβολισμός της γλυκερόλης στη ζύμη Yarrowia lipolytica και προοπτικές ανάπτυξης νέων βιοδιεργασιών
Μελετήθηκε ο μεταβολισμός της γλυκερόλης στη ζύμη Yarrowia lipolytica ACA–DC 50109 με έμφαση στη μετατροπή της σε λιπίδια και κιτρικό οξύ, μεταβολικά προϊόντα που παρουσιάζουν ιδιαίτερο ενδιαφέρον για τη βιοτεχνολογία. Σε καλλιέργειες που πραγματοποιήθηκαν σε βιοαντιδραστήρα διαλείποντος έργου, επί …
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Funktionelle Analyse und Charakterisierung des Gpr1-Proteins in der Hefe Yarrowia lipolytica
In der Hefe Yarrowia lipolytica führen Mutationen im GPR1-Gen zu Essigsäuresensitivität. Die Deletion dieses Genes hat demgegenüber keinen Effekt auf den Phänotyp. In dieser Arbeit wurde das Gpr1-Protein näher charakterisiert. Es zeigte sich, dass GPR1-Mutantenstämme wesentlich schneller Acetat …
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Die Nutzung der Hefe Yarrowia lipolytica zur Produktion von Citronensäure aus nachwachsenden Rohstoffen
… Nutzung interessanten Eigenschaften der Hefe Yarrowia (Y.) lipolytica ist ihr Vermögen, unter bestimmten Kultivierungsbedingungen große Mengen an organischen Säuren, darunter auch Citronensäure (CS), ins extrazelluläre Medium zu sekretieren. Aufgrund ihrer Apathogenität, ihres breiten …
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The development of a ribosome profiling protocol to study translation in the yeast Yarrowia lipolytica
… uncover new information about translation of the Yarrowia lipolytica transcriptome, an unusual yeast with interesting properties, with the aims of promoting its use as a candidate for biotechnology.
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Microbial oxidation of dodecane and tridecane into a,w-dicarboxylic acids using recombinant Yarrowia lipolytica
… cytochrome P450 hydroxylase complex. Recombinant Yarrowia lipolytica strains TVN 497, TVN 499, TVN 501 and TVN 502 were developed in such a manner by the collaborating research group at the University of the Free State and made available for this research.
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Funktionelle Analyse von Proteinen der Gpr1/Fun34/yaaH-Proteinfamilie in den Hefen Yarrowia lipolytica und Saccharomyces cerevisiae
Trans-dominante Mutationen im GPR1-Gen der Hefe Yarrowia lipolytica führen zur Sensitivität der Hefezellen gegenüber Essigsäure. Die Deletion dieses Genes hat dem gegenüber keinen Effekt auf den Phänotyp. In dieser Arbeit wurde das Gpr1-Protein aus Y. lipolytica und dessen Orthologe Ycr010cp, …
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Application of Single-Molecule Analytical Methods for the Characterization of Multimeric Genetic Elements in Yarrowia lipolytica DSM 3286
… related to mammals.<br /> The oleaginous yeast Yarrowia lipolytica was identified through a comprehensive review of the existing literature as such a potentially suitable model organism. Additionally, the yeast was confirmed as one of the most promising alternative expression systems to …
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Exploring volatile fatty acids (VFAs) as a novel substrate for microbial oil production
… studied fermentations of the oleaginous microbe Yarrowia lipolytica engineered for lipid overproduction. With acetate as sole carbon source, we conducted fed batch fermentations of Y. lipolytica in which acetic acid was maintained at low, non-inhibitory levels yielding high lipid titer of 50 g/L …
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Investigating the rate-limiting step of mitochondrial complex I catalysis
… of kinetic parameters of purified bovine and *Yarrowia lipolytica* complex I mutants were measured under a range of conditions. I find complex I robustly displays a solvent kinetic isotope effect (KIE), signifying that the rate-limiting step involves a proton transfer. This isotopic sensitive …
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Engineering Saccharomyces ceresisiae for the Secretion of an Extracellular Lipase
… the growth media. We selected the LIP2 gene from Yarrowia lipolytica, which encodes a nonspecific lipase. Several modifications were made to the LIP2 gene to improve processing. Results identified strains secreting the most lipase. From these results, high producing strains were inserted into an …
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Evaluation of microbial adjuncts and their effect on the ripening of cheddar cheese
… some individual free amino acids after 180 d. Yarrowia lipolytica DPC 6266, Yarrowia lipolytica DPC 6268 and Candida intermedia DPC 6271 were used to accelerate the ripening of Cheddar cheese. Strains were grown in YG broth to a final concentration of 107 cfu/ml, microfluidized, freeze-dried …
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The influence of yeasts on the aroma of Stilton cheese
… cheese aroma, increased as the concentration of Yarrowia lipolytica, inoculated into models containing P. rouqeforti, increased. Variation in the aroma profiles of replicate samples decreased as the concentration of Kluyveromyces lactis, inoculated into models containing P. roqueforti, decreased. …
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A dielectrophoretic, microfluidic device for sorting lipid-producing organisms used as bio-oil sources
… and low lipid phenotypes of the oleaginous yeast Yarrowia lipolytica using a 250 MHz, 2.6 Vp) signal in our device. Purity of the low lipid streams and high lipid streams are 98% and 71%, respectively. The high lipid stream purity can be improved by adjusting the spacing of the array. This work …
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Evaluation of the effect of growth conditions on lipid production by Papiliotrema laurentii UFV-1 in culture media containing xylose
… UFV-1, contrary to the oleaginous yeast model Yarrowia lipolytica, is capable of growing and accumulating lipids from xylose, the main sugar found in hemicellulose. Therefore, this work aimed to evaluate the effects of pH, C/N ratio, agitation speed, initial cell density and temperature on …
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Heterologous production of recombinant peptidylglycine α-amidating monooxygenase for the production of biosimilar α-amidated peptides
… expressed in the non-conventional yeast host, Yarrowia lipolytica. The novel fungal PHM's nutrient, temperature, and pH conditions were optimised to maximise protein expression. Enzyme purification was optimised with scalable industrial appropriate methods to purify milligram amounts of fungal …
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Developing synthetic, minimal promoters in Saccharomyces cerevisiae
… is demonstrated in a non-conventional yeast, Yarrowia lipolytica by assembling multiple native UAS in tandem with a core. In doing so, the strongest promoters ever reported for Y. lipolytica were created. Drawing from these lessons, the length of promoters in the popular host strain …
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