Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 20 for “"Y2H"”.
-
Identification of Artemis and PARP-1 interacting factors: A yeast two hybrid screening approach
… network. A series of yeast two hybrid screening (Y2H) experiments were conducted using both classical and interacting mating Y2H systems to find Artemis and PARP-1 interacting factors. The ATRX protein was identified as a putative interactor of Artemis, Caspase-7 and an "unkown" protein were …
-
Functional analysis of novel protein-protein interactions involving ROP GTPases in Arabidopsis thaliana and Populus trichocarpa
We are using the yeast two-hybrid (Y2H) system to identify novel protein-protein interactions (PPI) relevant to wood formation. Bait proteins for Y2H binary assays and screening against a xylem cDNA prey library were selected from approximately 400 Populus trichocarpa genes that are at least 8-fold …
-
Demonstration of Interactions Among Dif Proteins and the Identification of Kapb as a Regulator of Exopolysaccharide in Myxococcus Xanthus
… since investigated with the yeast two-hybrid (Y2H) system. The results indicate that DifC interacts with both DifA and DifE. Using a modified Y2H system, DifC was shown to be able to bring DifA and DifE into a protein complex. Further Y2H experiments demonstrated that the different conserved …
-
Discovery of New Protein-DNA and Protein-Protein Interactions Associated With Wood Development in Populus trichocarpa
… Further testing of PtrRAD1 by Yeast Two-Hybrid (Y2H) assay identified a protein-protein interaction (PPI) with poplar DIVARACATA RADIALIS INTERACTING FACTOR (DRIF1). PtrDRIF1 was then used in the Y2H assay and formed PPIs with MYB/SANT domain proteins, homeodomain family (HD) TFs, and …
-
Identification of host proteins targeted by the flax rust effector protein AvrL567
… of the effector proteins, Yeast-2-Hybrid (Y2H) screens were performed with cDNA from rust infected flax leaves. This approach allowed for the identification of plant targets as well as potential fungal cofactors. Eight putative interactors (iA1 to iA8) were isolated for AvrL567, none could …
-
Identification of host proteins targeted by the flax rust effector protein AvrL567
… of the effector proteins, Yeast-2-Hybrid (Y2H) screens were performed with cDNA from rust infected flax leaves. This approach allowed for the identification of plant targets as well as potential fungal cofactors. Eight putative interactors (iA1 to iA8) were isolated for AvrL567, none could …
-
MenzelJohannes_MSc_July_2013
… for these studies. First, a Yeast 2-Hybrid (Y2H) screen utilizing Apc5 as bait recovered the lifespan determinant Fob1 as a potential APC substrate. We hypothesized that the APC targets Fob1 for proteasome- and ubiquitin-dependent degradation. Authenticating Fob1 as a novel APC substrate …
-
Molekulare Charakterisierung des COPS5-Gens und seines Genproduktes als Kandidat für die Spastische Spinalparalyse
… verantwortlich ist. In einem Yeast Two Hybrid (Y2H) konnte die Interaktion seines Genprodukts Spastin mit COPS5 gezeigt werden. Ziel dieser Arbeit war deshalb eine Charakterisierung des COPS5-Gens und seines Genproduktes als mögliches Kandidaten-Gen für die HSP. Zuerst konnte das …
-
Structural And Functional Characterization Of Proteins Of Unknown Function (hp0495, Hp0100 And Hp1259) In Helicobacter Pylori
… and Hp0100 were identified by yeast two-hybrid (Y2H) as potential new players in tRNA aminoacylation and fidelity. By Y2H, Hp0495 showed interactions with EF-Tu and Hp0100 was connected to both ND-AspRS and AdT. The work presented in this dissertation examined the possible roles of both of these …
-
Caretaker-Gen-Syndrome
… Unter Verwendung des "Yeast Two Hybrid" (Y2H)-Systems konnten zunächst mehrere Interaktionen zwischen FA- und RAD51-Proteinen detektiert werden. Zur Bestätigung einer funktionellen Verbindung wurden die FA- und RAD51-Proteine in humanen 293-Zellen überexprimiert. Aufgrund der focibildenden …
-
Isolierung und Charakterisierung von Organisationsfaktoren des Vimentin-Filamentsystems
… des Vimentin-Filamentsystems mit Hilfe eines „Y2H“-Systems isoliert und charakterisiert. Als „Fänger“-Protein sollte die alpha-helikale zentrale Rod-Domäne von Vimentin, die den größten Teil des Moleküls darstellt und die im wesentlichen die Filamentoberfläche bildet, verwendet werden. Wir …
-
Characterization of Heat Shock Protein A12B as a Novel Angiogenesis Regulator.
… HspA12B. Through the use of a yeast two-hybrid (Y2H) system HspA12B was found to interact with a known angiogenesis regulator, <u>A</u> <u>K</u>inase <u>A</u>nchoring <u>P</u>rotein 12 (AKAP12). This interaction was confirmed by co-immunoprecipitation and by colocalization. In primary human …
-
The Arabidopsis C/S1 bacic leucine Zipper transcription factor network:Impact of heterodimer formation on target gene transcription
… den Daten aus dem klassischen Hefe two-hybrid (Y2H) verglichen (EHLERT et al., 2006). Die sehr spezifische Heterodimerisierung zwischen den neun Mitgliedern der Gruppe C und S1 bZIPs aus Arabidopsis thaliana spricht für eine funktionelle Bedeutung dieser, als C/S1 bZIP Netzwerk bezeichneten TF. …
-
The Protein-protein Interaction Map of the Treponema pallidum Flagellar Apparatus
… in B. subtilis) wurde näher untersucht. Die Y2H-Interaktionen von TP0658 mit Flagellinen (flaB1-3) wurden biochemisch verifiziert. TP0658 bindet an eine C-terminale Sequenz von FlaB1 zwischen L231 und D247 (dem so genannten C-Loop der Flagelline). Dieses epitop ist in B. subtilis und anderen …
-
Characterising the role of GPR50 in neurodevelopment and lipid metabolism
… demonstrated in this thesis. Verification of the Y2H study suggested GPR50 does not physically interact with SREBF2 or ABCA2. However, ABCA2 appears to induce the intracellular localisation of GPR50 in several cell lines. In SH-SY5Y cells, this was mimicked by the inhibition of cholesterol …
-
Investigating the structure and function of HSV-1 tegument proteins: UL7 and UL51
… of the centriole protein CPAP was identified by Y2H screen, and validated by immunoprecipitation from transfected cells and in pull-down experiments using recombinant proteins purified from E. coli. The CPAP-binding region within UL51 was mapped and shown to resemble a motif present in the …
-
The essentiality of DivIVAEf oligomerization for proper cell division in enterococcus faecalis and interaction with a novel cell division protein
… protein, MLJD1, by screening a Yeast Two-Hybrid (Y2H) was weak. GST-pulldown and immunoprecipitation did indicate DivIVAEf interaction with MLJD1, but another in vivo assay was required to support these results. In this study I demonstrate a strong interaction, using an in vivo Bacterial …
-
The Functional Characterization of the LysR-Type Transcriptional Regulator QseD and the SorC-Type Transcriptional Regulator LsrR in Enterohemorrhagic Escherichia coli
… order to bind DNA, a targeted yeast-two-hybrid (Y2H) approach was used to exclude the known LTTR regulators of LEE transcription QseA and LrhA, as QseD interaction partners. Taken together, these results show that QseD regulates alternate targets in EHEC and K-12 E. coli, and that EHEC O157:H7 …
-
Unraveling the Structure and Assessing the Quality of Protein Interaction Networks with Power Graph Analysis
Molecular biology has entered an era of systematic and automated experimentation. High-throughput techniques have moved biology from small-scale experiments focused on specific genes and proteins to genome and proteome-wide screens. One result of this endeavor is the compilation of complex networks …