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Showing 1 to 20 of 29 for “"Xenopus oocytes"”.
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Dynamics of Germ Cell Specific Stable Transcriptional Complexes in Xenopus Oocytes
… mRNAs or DNAs into the non-dividing oocyte of Xenopus. Transcription factor proteins can be introduced by mRNA injection and DNA sequences to which they bind, when required; induced gene expression is monitored by expression of fluorescent reporter genes. I have used competition experiments …
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Functional characterization of a putative Plasmodium falciparum calcium/hydrogen antiporter pfcha
… of this putative calcium hydrogen antiporter in Xenopus oocytes and in a S. cerevisiae strain which is deficient for the yeast vacuolar calcium hydrogen antiporter VCX1. The putative 441-residue protein, which we designate PfCHA, is predicted to contain 11 membrane-spanning segments and is …
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The role of transcription factors in somatic cell nuclear reprogramming by eggs and oocytes
… transcription factors during SCNR by Xenopus oocytes, which are inactive in DNA replication and cell division. QPCR measures changes in the steady-state levels of transcripts within 2 days of nuclear transfer to Xenopus oocytes (Oocyte-NT). Three pairs of Yamanaka factor homologs were …
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Untersuchung und Spezifizierung der molekularen Interaktion von Ubiquitin und Sumo mit dem rekombinanten a1-Glyzinrezeptor
… expressed as radioactive His-tagged protein in Xenopus oocytes. After incubation it was expected that adducts of ubiquitin/sumo with the receptor could be isolated by using GST-pullover or Ni2+-NTA-Chromatographie. However, there were no adducts found. In further experiments the …
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Developmental and estrogen regulation of Xenopus laevis retinol-binding protein gene expression
… protein and estrogen receptor genes in Xenopus embryonic livers has been examined. Serum retinol binding protein (RBP) mRNA is detected exclusively in liver tissue in both adult Xenopus laevis and in tadpoles. RBP mRNA is absent in Xenopus oocytes and is present at extremely low levels …
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Molecular characterization of cation-coupled transporters: the H+-coupled Mg2+-citrate transporter, CitM, and the Na+/sulfate cotransporter, hNaSi-1
… and mutants were functionally expressed in Xenopus oocytes. Antibodies against hNaSi-1 were used to investigate tissue distribution and N-glycosylation. The roles of two conserved serine residues in the transport function of hNaSi-1 were investigated using site-directed mutagenesis and …
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The Lipid-Facing M4 Helix of α4β2 nACh and 5-HT3A Receptors
… of mutant receptors in HEK293 cells and Xenopus oocytes, and radioligand binding and immunofluorescence to assess ligand binding and receptor expression. I show that 1 out of 28 alanine mutations in the 5-HT3AR M4 and 8 out of28 double alanine mutations in the α4β2 nAChR abolish receptor …
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A molecular characterization of agonists that bind to Hco-UNC-49, a GABA-gated chloride channel from Haemonchus contortus
… receptor agonists on Hco-UNC-49 expressed in Xenopus oocytes. The results of our electrophysiological recordings indicate that there is a size requirement for full agonism of the Hco-UNC-49 binding site. Furthermore, a number of molecules that are known to act on vertebrate GABA receptors have …
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G Protein and Androgen Signaling in Ovarian Function
Oocytes are held in meiotic arrest in prophase I until ovulation when gonadotropins trigger a subpopulation of oocytes to resume meiosis in a process termed "maturation." Meiotic arrest is maintained through a mechanism whereby constitutive cAMP production exceeds phosphodiesterase-mediated …
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Poly(A)+ RNA From Sheep Omasal Epithelium Induces Expression of a peptide Transport Protein(S) in Xenopus laevis Oocytes
… from sheep omasal epithelium was injected into Xenopus laevis oocytes. Poly(A)+ RNA was functionally expressed in Xenopus oocytes 4 to 7 d post-injection. Peptide (5 di-, 10 tri-, 6 tetra-, 2 penta-, 1 hepta-, 1 septa-, 1 octapeptide) transport capability was measured by impaling oocytes with a …
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With No Lysine 1 (WNK1): A Potential Regulator of The Lysosomal Degradation Pathway
… groups have used reconstitution assays in Xenopus oocytes and mammalian cell lines to show WNKs regulate the surface expression and/or activity of various ion transporters and channels, including the epithelial sodium channel (ENaC) and the sodium chloride co-transporter (NCCT). Although …
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Mutations in Connexin43 and Connexin40 Associated with Human Atrial Fibrillation; Physiology and Mechanism
… the Cx40-F30L mutant, leading to cell death in Xenopus oocytes. Increased sensitivity to intracellular pH caused closure of gap junction channels composed of Cx40-G311S or Cx40-R113S. Intracellular localization of a previously published Cx40 mutation (P88S) was determined to be retained within …
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Design, Synthesis and Biological Evaluation of a Family of Excitatory Amino Acid Transporter 3 (EAAT3) Preferring Inhibitors
… recordings of EAAT-mediated currents in Xenopus oocytes further identified L-β-BA as a non-substrate inhibitor. Derivatives of L-β-BA were prepared and characterized for the ability to inhibit 3H-D-aspartate uptake into hEAAT1-3 expressing C17.2 cells. Computational modeling and …
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Design, Synthesis and Biological Evaluation of a Family of Excitatory Amino Acid Transporter 3 (EAAT3) Preferring Inhibitors
… recordings of EAAT-mediated currents in Xenopus oocytes further identified L-β-BA as a non-substrate inhibitor. Derivatives of L-β-BA were prepared and characterized for the ability to inhibit 3H-D-aspartate uptake into hEAAT1-3 expressing C17.2 cells. Computational modeling and …
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THE ROLE OF A AND B VITAMINS DURING OROFACIAL DEVELOPMENT OF XENOPUS LAEVIS
… defects (Weingartner, Lotz et al. 2007). Xenopus laevis, and the closely related Xenopus tropicalis, are excellent model systems for orofacial development studies. The ease of embryo collection and manipulation, in addition to the conservation of DNA sequence between the two species, makes …
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Molecular Cloning and Functional Characterization of a Turkey Intestinal Peptide Transporter (tPepT1), and Developmental Regulation of PepT1 Expression in Turkey and Broiler Embryos
… Using a two-electrode voltage-clamp technique in Xenopus oocytes expressing tPepT1, Gly-Sar transport was pH dependent, but independent of Na+ and K+. For the dipeptides Gly-Sar and Met-Met, the evoked inward currents indicated that the transporter was saturable and had a high affinity for these …
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Molecular Cloning, In Vitro Expression, and Functional Characterization of an Ovine Gastrointestinal Peptide Transporter (oPepT1)
… of oPepT1 was assessed by expressing oPepT1 in Xenopus oocytes using a two-electrode voltage-clamp technique. Overall, the in vitro transport characteristics of oPepT1 expressed in oocytes were similar to those of PepT1 from other species. The transport process is electrogenic and pH-dependent, …
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Cloning, Expression, and Developmental and Dietary Regulations of a Chicken Intestinal Peptide Transporter and Characterization and Regulation of an Ovine Gastrointestinal Peptide Transporter Expressed in a Mammalian Cell Line
… were used for functional analysis of cPepT1 in Xenopus oocytes and Chinese hamster ovary (CHO) cells. For most di- and tripeptides tested, the Kt was in the micromolar range, except Lys-Lys and Lys-Trp-Lys. Northern analysis demonstrated that cPepT1 is expressed strongly in the small intestine, …
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Functional characterisation of the honeybee Apis mellifera and diamondback moth Plutella xylostella α5 nicotinic acetylcholine receptor identifies a subset of ion channels with unusual pharmacological properties
… expresses as a homomeric ion channel in Xenopus laevis oocytes that shows unusual sensitivity to biogenic amines including dopamine whilst being insensitive to acetylcholine. We have taken advantage of this to create mutations in the ligand-binding site of α5 to identify amino acid …
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Using Xenopus laevis to study the mechanisms governing the early germline development and the formation of vertebrate body plan
… like Drosophila, C. elegans, zebrafish and Xenopus, the primordial germ cells (PGC), the precursors of the gametes, are specified through the inheritance of germ plasm. Early PGC development is regulated by the genetic program coded by unique maternal factors in the germ plasm. However, the …
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