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Showing 1 to 20 of 22 for “"Xenopus laevis oocytes"”.

  1. Structure-Function Studies on Human Epithelial Cystic Fibrosis Chloride Channels Expressed in Xenopus Laevis Oocytes

    Csanady, Laszlo

    <p>The cystic fibrosis transmembrane conductance regulator (CFTR) Cl<sup>-</sup> channel is an ATP-binding cassette protein, comprising two transmembrane domains, two nucleotide binding domains (NBD1, NBD2) and a regulatory (R) domain. Channel gating is controlled by R-domain phosphorylation and by …

    rockefeller Repository record

  2. Poly(A)+ RNA From Sheep Omasal Epithelium Induces Expression of a peptide Transport Protein(S) in Xenopus laevis Oocytes

    Pan, YuanXiangVirginia Tech1996

    … from sheep omasal epithelium was injected into Xenopus laevis oocytes. Poly(A)+ RNA was functionally expressed in Xenopus oocytes 4 to 7 d post-injection. Peptide (5 di-, 10 tri-, 6 tetra-, 2 penta-, 1 hepta-, 1 septa-, 1 octapeptide) transport capability was measured by impaling oocytes with a …

    vt Repository record

  3. Molecular characterization of GABA receptor subunits from the parasitic nematode Haemonchus contortus

    Siddiqui, SalmaUniversity of Ontario Institute of Technology2009

    … subunit forms a functional homomeric channel in Xenopus laevis oocytes that produces a robust response to GABA and is highly sensitive to picrotoxin. In contrast, Hco-UNC-49C alone does not respond to GABA but can assemble with Hco-UNC-49B to form a heteromeric channel with an increased …

    uoit Repository record

  4. Investigation of an unusual GABA-A receptor: studies on the promiscuous ε [epsilon] subunit

    Schwabe, TNottingham Trent University2010

    … the α3, β2 and ε subunits were examined in Xenopus laevis oocytes using the two-electrode voltage-clamp technique. The results obtained confirm that the ε subunit confers unusual properties on the GABAA receptor.

    nott-trent Repository record

  5. Functional characterization of nitrate transporters in maize.

    Wen, Zhengyu2015

    … using a preliminary screening experiment in Xenopus laevis oocytes. Combining electrophysiology and chemical flux analysis, ZmNPF6.4 was characterized as a pH-dependent, low-affinity, non-selective nitrate and chloride transporter. On the other hand, ZmNPF6.6 encoded a pH-dependent, …

    adelaide Repository record

  6. Demonstration of peptide and free amino acid absorption by sheep forestomach epithelium using parabiotic chambers and identification of H⁺/peptide and free amino acid transport proteins in sheep omasal epithelium and b<sup>o,+</sup> amino acid transport proteins in pig jejunal epithelium by expression of mRNA in Xenopus laevis oocytes

    Matthews, James ClydeVirginia Tech1995

    … to express exogenous mRNA in defolliculated Xenopus laevis oocytes was developed using sucrose gradient size-fractionated poly(A)⁺ RNA (RNA) isolated from the jejunal epithelial tissue of pig (average BW = 33.8 kg). Compared to water-injected oocytes, RNA injected oocytes displayed greater …

    vt Repository record

  7. G Protein and Androgen Signaling in Ovarian Function

    Deng, James Yang2010

    Oocytes are held in meiotic arrest in prophase I until ovulation when gonadotropins trigger a subpopulation of oocytes to resume meiosis in a process termed "maturation." Meiotic arrest is maintained through a mechanism whereby constitutive cAMP production exceeds phosphodiesterase-mediated …

    utswmed Repository record

  8. Identification and functional characterisation of a class on non-selective cation channels in Saccharomyces cerevisiae.

    Carter, Scott Anthony William2009

    … and electrophysiological examination using Xenopus laevis oocytes revealed these proteins catalyse the non-selective flux of mono-valent cations following unsaturable kinetics, indicative of a low affinity transport system. Further analysis revealed this cation flux is sensitive to external …

    adelaide Repository record

  9. Identification and characterization novel of cystine-loop ligand- gated chloride channels from Dirofilaria immitis: pharmacological analysis and novel compound screening

    Varley, SierraUniversity of Ontario Institute of Technology2023

    … channels through heterologous expression in Xenopus laevis oocytes. Levamisole derivatives were therefore tested on H. contortus ACC-2, which identified at least one showing a higher sensitivity compared to levamisole. Overall, this study characterized 2 novel UNC-49 receptors, identified 3 …

    uoit Repository record

  10. Engineering and functional characterisation of pentameric concatenated (alpha 4)2 (beta 2)3 and (alpha 4)3 (beta 2)2 nicotinic acetylcholine receptors

    Carbone, A LOxford Brookes University2009

    … engineered. These receptors were expressed in Xenopus laevis oocytes and functional studies showed that their functional properties resembled those of their non-linked counterparts. Subsequent site-directed mutagenesis in combination with functional analysis allowed the identification of the …

    oxford-brookes Repository record

  11. Structure-function studies of human SLC36 amino acid transporters

    Pillai, Samyuktha MuralidharanOxford Brookes University2011

    … and tryptophan transporter when expressed in the Xenopus laevis oocyte system. Preliminary data for hPAT3 suggested uptake of proline at extracellular pH 7.4 in Xenopus laevis oocytes and in the mouse Sertoli cell line TM4. Membrane topology studies on hPAT1 provided experimental support for the …

    oxford-brookes Repository record

  12. Functional characterisation of aphid, Myzus persicae, nicotinic acetylcholine receptors and identification of a novel biogenic amine-gated ion channel from the honeybee, Apis mellifera

    Mitchell, Eleanor LouiseOxford Brookes University2022

    … The heterologous expression of nAChRs, in Xenopus laevis oocytes, has enabled the study of functional and pharmacological properties of these receptors by two-electrode voltage clamp electrophysiology. Myzus persicae, the peach potato aphid, is a major global pest of multiple crop species. …

    oxford-brookes Repository record

  13. Chlorellavirus-Proteine: I. Die Hüllprotein-Genfamilie II. Ein primitiver Kaliumkanal

    Ebert, Barbara2012

    … dependent, potassium-selective conductance in Xenopus laevis oocytes, which is inhibited by Ba2+ and amantadine, but not Cs+. Virus replication is inhibited in the same fashion, which indicates a role of Kcv in the viral life cycle. Thus, Kcv is the first potassium channel which is encoded by a …

    goettingen Repository record

  14. A genomic analysis using RNA-Seq to investigate the adaptation of the psychrophilic diatom Fragilariopsis cylindrus to the polar environment

    Strauss, JanUniversity of East Anglia2012

    … iron starvation and heterologous expression in Xenopus laevis oocytes experimentally confirmed light-driven proton pumping for the iron-induced rhodopsin allele, suggesting significance for the adaptation of F. cylindrus to environmental conditions of the Southern Ocean. These data show how the …

    east-anglia Repository record

  15. Characterization of a human renal organic anion transporter

    Reid, Glen2012

    … To enable heterologous expression in the Xenopus laevis oocyte system, an hROAT1 expression clone was constructed by subcloning the hROAT1 reading frame between the 5' and 3' UTRs of the flounder sodium dicarboxylate cotransporter, fNaDC-3. Expressed in Xenopus laevis oocytes, hROAT1 …

    goettingen Repository record

  16. A Study of Cellular Amino Acid Homeostasis Identifies Novel Targets, Inhibitors and Combination Therapies in Cancer

    Gauthier-Coles, Gregory2022

    … paralog of SNAT2, called SNAT4, when assayed in Xenopus laevis oocytes. Nevertheless, its cytotoxicity was tested in cancer cells, and it was found to be well tolerated at low micromolar concentrations. However, when combined with the glucose transport inhibitor Bay876 synergistic effects …

    aus-cath Repository record

  17. A Study of Cellular Amino Acid Homeostasis Identifies Novel Targets, Inhibitors and Combination Therapies in Cancer

    Gauthier-Coles, Gregory2022

    … paralog of SNAT2, called SNAT4, when assayed in Xenopus laevis oocytes. Nevertheless, its cytotoxicity was tested in cancer cells, and it was found to be well tolerated at low micromolar concentrations. However, when combined with the glucose transport inhibitor Bay876 synergistic effects …

    anu Repository record

  18. Computational modelling of the glycinergic synapse

    Al-Saidi, ElzbietaOxford Brookes University2020

    … at position Ser 407 on GlyT1 expressed in Xenopus laevis oocytes. The mutation lead to the inhibition of transport via the GlyT1 as compared to wild type transporter, and the result was integrated into the kinetic synapse model suggesting that the loss of GlyT1 function leads to a higher …

    oxford-brookes Repository record

  19. Structure-function studies of SLC15 peptide transporters

    Senan, AnishOxford Brookes University2015

    … for PepT1 mediated transport in PepT1 expressing Xenopus laevis oocytes and differentiated Caco-2 cells grown on Transwell® inserts. The cLu- carrier was found to exhibit higher binding affinity towards PepT1 compared to Aib- and Thio- carriers and to transport the conjugated drugs both in the …

    oxford-brookes Repository record

  20. Functional Characterization of the Mammalian TRPV4 Channel: Yeast Screen Reveals Gain-of-Function Mutations

    Doyle, Christina2012

    … their activity when heterologously expressed in Xenopus laevis oocytes. Of the subset of mammalian TRP channels tested, only wild type TRPV4 rescued the ability of the yeast strain trk1Δ trk2Δ to grow on low potassium media. The TRPV4 channel is important in thermosensitive, osmosensitive, and …

    columbia-diss Repository record

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