Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"XRN1"”.
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Assessing and understanding the generation and function of RNA decay intermediates in non-insect borne flaviviruses
… major cytoplasmic cellular 5'-3' decay enzyme XRN1. In addition, members of the generas Hepacivirus, Hepatitis C Virus (HCV) and Pestivirus, Bovine Viral Diarrhea Virus (BVDV), possess XRN1 stalling elements within their 5' UTRs. These stalling sites block the action of the exonuclease and …
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Translation of Partially Decayed Messenger RNAs in Yeast
… that halt the progression of 5’→3’ exonuclease Xrn1. We show that these viral Xrn1-resistant structures (xrRNAs) can be used to protect specific homologously-expressed messenger RNAs from 5’→3’ degradation. We investigated the effects of addition of xrRNAs, artificially-installed into the …
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Point mutational examination of eukaryotic initiation factor 4E (eIF4E), inclusion bodies analysis, and Barley Yellow Dwarf Virus (BYDV) exonuclease resistant RNA structure mapping and prediction
… dwarf virus RNA (BYDV) that blocks exonuclease Xrn1 to generate BYDV subgenomic RNA3. Deletion analysis places the Xrn1 resistant (xrRNA) structure within the first 5’ 67 nucleotides of BYDV sgRNA3. Dianthoviruses, related to luteoviruses, have recently been shown to contain an xrRNA structure …
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Functions of Dcp2 and Ski7 In Mrna Degradation
… the 5` <sup>m7</sup>G cap of mRNAs, allowing Xrn1 to degrade the mRNA from the 5` end. Alternatively, mRNA is degraded from the 3` end by the RNA exosome.</p> <p>While decapping by Dcp2 is a critical step in mRNA decay, its physiological function has been unclear. Null mutations of …
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Computational and experimental detection of uncoupling between transcriptome and translatome changes of gene expression
… post-transcriptional regulation pathways: 4E-T, Xrn1 and Dicer.
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The Roles of Rnt1 and Putative Endoribonucleases in Eukaryotic mRNA Degradation
… to the cytoplasm for further degradation by Xrn1 and that Rnt1 may play a role in regulating gene expression. In fact, several mRNAs rely heavily on Rnt1 for turnover. To further explore the biological relevance of Rnt1 mRNA cleavage, I performed mutational analysis and experimental evolution …
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Stepwise Reconstitution of RNA Cellular Activity
… unique targetable motifs – the Zika virus (ZIKV) Xrn1-exonuclease resistant RNA element (xrRNA1). Using a pipeline consisting of structure-based virtual screening and experimental screening of viral attenuation and cellular function, I screened ~80,000 small molecules and found eight that …