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Showing 1 to 10 of 10 for “"XPD"”.
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XPD Helicase: Shifting the Inchworm Into Reverse
… processing machines. Here, I used the archaeal XPD helicase to answer these four fundamental questions. This work characterizes XPD as a 5' to 3' ssDNA translocase that preferentially unwinds forked DNA substrates, contains an FeS cluster, binds to the ssDNA-dsDNA junction of forked substrates, …
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Biological and functional consequences of polymorphisms in the XPD gene
… (SNPs) in the nucleotide excision repair gene XPD (ERCC2) and cancer risk. Little is known, however, about the underlying mechanisms for these associations. I used lymphocytes from healthy subjects to explore novel mechanisms which could explain the reported risk-modifying effects on disease …
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Direct observation of XPD helicase base-pair stepping and regulation by RPA2
… and fluorescence microscopy to decipher how FacXPD helicase is modulated by FacRPA2. FacXPD is the archaeal homolog of yeast Rad3 and human xeroderma pigmentosum group D protein (XPD) helicase from the organism Ferroplasma acidarmanus. This enzyme serves as a model for understanding the …
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Single-molecule optical trapping studies of the regulation mechanisms of XPD helicase
… repair helicase Xeroderma pigmentosum group D (XPD), an iron-sulfur (Fe-S) cluster-containing helicase with medical relevance. XPD plays a critical role in DNA repair, specifically nucleotide excision repair (NER), and functions as part of the large TFIIH complex in eukaryotes. However, its …
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The effect of single-stranded DNA binding protein RPA2 on XPD helicase processivity
… in DNA repair. Xeroderma pigmentosum group D (XPD) is a helicase protein that plays an important role in nucleotide excision repair (NER). Its function is to unwind double-stranded DNA, allowing access to the bases that connect the strands and code genetic information. Previous work has shown …
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Expeditious detection of DNA damage: Probing the sequence-sensing and damage-sensing mechanism(s) of the XPD helicase
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-05-01
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Direct real-time correlation of protein conformation and substrate recognition
… DNA substrates. The strategy was applied to XPD, an iron-sulfur (FeS) cluster-containing DNA repair protein. Conformational dynamics was assessed via FeS-mediated quenching of a fluorophore site-specifically incorporated into XPD. Simultaneously, binding of DNA molecules labeled with a …
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Tailored chemotherapy based on genomic polymorphisms for metastatic colorectal cancer treated with 5-fluorouracil plus oxaliplatin or irinotecan in Korean patients
… 분석하였다. PCR-based RFLP 방법을 사용하여 TS, MTHFR-977, XPD-751, GSTP1-105, XRCC1-399, UGT1A1*28 유전자에 대해 분석한 후 다형성에 따라 약제를 선택하여 항암화학치료를 시행하였다. 3주기 시행 후 반응도를 평가하였다. 결과: 44명의 환자가 연구에 참여되었으며 32명의 환자에서 FOLFOX용법으로, 12명의 환자에서 FOLFIRI용법으로 치료받았다. 전체 환자에서 완전관해는 없었으며 맞춤화학치료에 대한 반응율은 43.2%(19명)였다. 유전자 다형성에서 …
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Single-molecule study of the DNA helicase regulating genomic stability
… fluorophore in distance dependent manner, XPD helicase translocation on ssDNA was monitored and, the differential effect of single-strand binding protein, RPAs on the XPD translocation kinetics were evaluated. Lastly, I aim to observe translocating motor on a physiological length of ssDNA. …
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Einfluss der Immunsuppressiva Cyclosporin A und Everolimus auf die funktionelle DNA-Reparaturfähigkeit sowie auf die Regulation von DNA-Reparatur-Genen
… A verminderte die mRNA-Expression von XPB, XPC, XPD und XPG. Auf Proteinebene führte Cyclosporin A lediglich zu einer reduzierten XPA- und XPG-Proteinexpression. Durch Überexpression von XPA und XPG in normalen und XP-defizienten Fibroblastenzelllinien konnte die funktionelle Bedeutung dieser …