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Showing 1 to 11 of 11 for “"XPD"”.
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XPD Helicase: Shifting the Inchworm Into Reverse
… processing machines. Here, I used the archaeal XPD helicase to answer these four fundamental questions. This work characterizes XPD as a 5' to 3' ssDNA translocase that preferentially unwinds forked DNA substrates, contains an FeS cluster, binds to the ssDNA-dsDNA junction of forked substrates, …
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Biological and functional consequences of polymorphisms in the XPD gene
… (SNPs) in the nucleotide excision repair gene XPD (ERCC2) and cancer risk. Little is known, however, about the underlying mechanisms for these associations. I used lymphocytes from healthy subjects to explore novel mechanisms which could explain the reported risk-modifying effects on disease …
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Direct observation of XPD helicase base-pair stepping and regulation by RPA2
… and fluorescence microscopy to decipher how FacXPD helicase is modulated by FacRPA2. FacXPD is the archaeal homolog of yeast Rad3 and human xeroderma pigmentosum group D protein (XPD) helicase from the organism Ferroplasma acidarmanus. This enzyme serves as a model for understanding the …
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Single-molecule optical trapping studies of the regulation mechanisms of XPD helicase
… repair helicase Xeroderma pigmentosum group D (XPD), an iron-sulfur (Fe-S) cluster-containing helicase with medical relevance. XPD plays a critical role in DNA repair, specifically nucleotide excision repair (NER), and functions as part of the large TFIIH complex in eukaryotes. However, its …
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The effect of single-stranded DNA binding protein RPA2 on XPD helicase processivity
… in DNA repair. Xeroderma pigmentosum group D (XPD) is a helicase protein that plays an important role in nucleotide excision repair (NER). Its function is to unwind double-stranded DNA, allowing access to the bases that connect the strands and code genetic information. Previous work has shown …
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Expeditious detection of DNA damage: Probing the sequence-sensing and damage-sensing mechanism(s) of the XPD helicase
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-05-01
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Direct real-time correlation of protein conformation and substrate recognition
… DNA substrates. The strategy was applied to XPD, an iron-sulfur (FeS) cluster-containing DNA repair protein. Conformational dynamics was assessed via FeS-mediated quenching of a fluorophore site-specifically incorporated into XPD. Simultaneously, binding of DNA molecules labeled with a …
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Tailored chemotherapy based on genomic polymorphisms for metastatic colorectal cancer treated with 5-fluorouracil plus oxaliplatin or irinotecan in Korean patients
… 분석하였다. PCR-based RFLP 방법을 사용하여 TS, MTHFR-977, XPD-751, GSTP1-105, XRCC1-399, UGT1A1*28 유전자에 대해 분석한 후 다형성에 따라 약제를 선택하여 항암화학치료를 시행하였다. 3주기 시행 후 반응도를 평가하였다. 결과: 44명의 환자가 연구에 참여되었으며 32명의 환자에서 FOLFOX용법으로, 12명의 환자에서 FOLFIRI용법으로 치료받았다. 전체 환자에서 완전관해는 없었으며 맞춤화학치료에 대한 반응율은 43.2%(19명)였다. 유전자 다형성에서 …
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Single-molecule study of the DNA helicase regulating genomic stability
… fluorophore in distance dependent manner, XPD helicase translocation on ssDNA was monitored and, the differential effect of single-strand binding protein, RPAs on the XPD translocation kinetics were evaluated. Lastly, I aim to observe translocating motor on a physiological length of ssDNA. …
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DNA repair genetic polymorphisms and breast cancer in the Portuguese population
… of different polymorphisms ( XRCC1 Arg399Gln, XPD Lys751Gln, RAD51 G135C, XRCC3 Thr241Met, TP53 Arg72Pro and TP53 PIN3 Ins16bp) belonging to the DNA damage signalling and repair mechanisms with breast cancer susceptibility, in familial and sporadic breast cancer, in the Portuguese population. …
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Einfluss der Immunsuppressiva Cyclosporin A und Everolimus auf die funktionelle DNA-Reparaturfähigkeit sowie auf die Regulation von DNA-Reparatur-Genen
… A verminderte die mRNA-Expression von XPB, XPC, XPD und XPG. Auf Proteinebene führte Cyclosporin A lediglich zu einer reduzierten XPA- und XPG-Proteinexpression. Durch Überexpression von XPA und XPG in normalen und XP-defizienten Fibroblastenzelllinien konnte die funktionelle Bedeutung dieser …