Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Wiskott-Aldrich Syndrome Protein"”.
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Biochemical and Biophysical Characterization of the Allosteric Equilibrium of the Wiskott-Aldrich Syndrome Protein
Proteins provide the essential building blocks of signal transduction pathways governing many biological processes. They are dynamic entities endowed with properties that allow them to respond to changes in the cell while maintaining specificity and fidelity of signaling in a crowded intracellular …
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Functions of Phosphatidylinositol 4-Phosphate 5 Kinases in Actin Cytoskeletal Regulation During Phagocytosis
… attachment but inhibits ingestion by decreasing Wiskott-Aldrich syndrome protein (WASP) activation and hence actin polymerization, in the nascent phagocytic cup. In addition, PIP5Kg but not a is transiently activated by spleen tyrosine kinase (Syk)-mediated phosphorylation. I propose that PIP5Kg …
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Processive Acceleration of Actin Barbed End Assembly by N-WASP
… processes including cellular migration. Neural Wiskott Aldrich syndrome protein (N-WASP) is involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. N-WASP activated actin polymerization drives extension of invadopodia and podosomes into the basement …
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Functional Characterization of Amphiphysin in Drosophila melanogaster
Amphiphysin (Amph) is a multi-domain protein that has been implicated in synaptic vesicle (SV) endocytosis. In vertebrates, Amph1 associates with SVs and binds to known endocytic proteins, such as dynamin and clathrin. Overexpression of the vertebrate Amph1 SH3 domain is sufficient to inhibit SV …
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The Role of FAM21 in the Regulation of Mitochondrial Dynamics
… machinery, including the actin-related protein 2/3 (Arp2/3)-complex, have been implicated at different stages of the complex multi-step mechanism of mitochondrial division. Recent work has also highlighted the contribution of phosphatidylinositol 4-phosphate (PI4P) trafficking, from …
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Study of T cell activation and migration at the single-cell and single-molecule level
… cells. The same testing procedure was applied to Wiskott-Aldrich syndrome protein (WASp)-deficient T cells that exhibit abnormal morphology and impaired chemotaxis. The stiffness of the diseased cells in the naive state was 1.5 times less than that of the non-diseased cells, a result that may be …
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Sensory Organ Morphogenesis in Caenorhabditis Elegans
… important for compartment morphogenesis and both proteins line the glial sensory compartment. LIT-1 compartment localization is important for its function and requires neuronal signals. Furthermore, the conserved LIT-1 C-terminus is necessary and sufficient for this localization. Two-hybrid and …
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Spatiotemporal Control of Actin Cytoskeletal Machinery in Cells: Multivalency Mediated Protein Clustering at the Plasma Membrane and Optogenetic Tool Development
… membrane is organized into distinct lipid and protein microdomains. Recent work has shown the importance of protein assembly at the membrane in the formation of these domains. However, the mechanisms by which proteins organize at the membrane are largely unknown. Here I demonstrate that …
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Biochemical Reconstitution and Functional Characterization of the WAVE Regulatory Complex
Members of the Wiskott-Aldrich syndrome protein (WASP) family (WASP, N-WASP, WAVE 1-3) have a central role in the transmission of the extracellular signals to the actin cytoskeleton. These proteins use their C-terminal VCA domain to stimulate the actin-nucleating activity of Arp2/3 complex in …
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Regulation of WASP in Actin Signaling: From Angstroms to Microns
Wiskott-Aldrich syndrome protein (WASP) regulates membrane-attached force generation by promoting actin polymerization, which is key to cell motility and immune responses. Mutations in WASP lead to Wiskott-Aldrich syndrome and neutropenia. My graduate research was focused on allosteric regulation …
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Investigating the Function of an Actin Nucleation-Promoting Factor N-WASP in Mammalian Myoblast Fusion
Pages 61-89 are misnumbered as pages 75-103, and pages 90-100 are misnumbered as pages 102-112.
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Phase Transitions of Multivalent Adaptor Proteins
… are self-assembled entities of lipids, proteins and carbohydrates. Additionally, within and on surface of membranes, molecules cluster into signaling compartments in many different biological pathways. Interactions between individual biomolecules have been studied comprehensively in …