Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Wiskott-Aldrich Syndrome"”.
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Natural and Exogenous Genome Editing In Wiskott-Aldrich Syndrome Patient Cells
<p>Wiskott-Aldrich syndrome (WAS) is an X-linked primary immunodeficiency disease characterized by thrombocytopenia, recurrent infections and increased autoimmunity. This disease is caused by mutations in the WAS gene (<em>WAS</em>) which encodes for the WAS protein (WASp), exclusively expressed in …
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Biochemical and Biophysical Characterization of the Allosteric Equilibrium of the Wiskott-Aldrich Syndrome Protein
… can examine the regulation and activation of the Wiskott-Aldrich Syndrome protein (WASP) by the Rho-family GTPase Cdc42. Results from this work revealed that a simple two-state model can predict the hydrogen exchange behavior and binding affinity for Cdc42-GTP of WASP proteins over a range of …
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Regulation of WASP in Actin Signaling: From Angstroms to Microns
Wiskott-Aldrich syndrome protein (WASP) regulates membrane-attached force generation by promoting actin polymerization, which is key to cell motility and immune responses. Mutations in WASP lead to Wiskott-Aldrich syndrome and neutropenia. My graduate research was focused on allosteric regulation …
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Functions of Phosphatidylinositol 4-Phosphate 5 Kinases in Actin Cytoskeletal Regulation During Phagocytosis
… attachment but inhibits ingestion by decreasing Wiskott-Aldrich syndrome protein (WASP) activation and hence actin polymerization, in the nascent phagocytic cup. In addition, PIP5Kg but not a is transiently activated by spleen tyrosine kinase (Syk)-mediated phosphorylation. I propose that PIP5Kg …
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Processive Acceleration of Actin Barbed End Assembly by N-WASP
… processes including cellular migration. Neural Wiskott Aldrich syndrome protein (N-WASP) is involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. N-WASP activated actin polymerization drives extension of invadopodia and podosomes into the basement …
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Functional Characterization of Amphiphysin in Drosophila melanogaster
… partner was the actin-nucleating protein, Wiskott Aldrich Syndrome Protein, WASP. Although WASP and Amph could be coimmunoprecipitated in vitro, WASP was not expressed at the t-tubule membrane, and Wasp mutants had normal t-tubule morphology. Clearly, Amph is essential for normal t-tubule …
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The Role of FAM21 in the Regulation of Mitochondrial Dynamics
… protein of the nucleation-promoting factor Wiskott-Aldrich Syndrome protein family member WASH (WASH) complex, recruits WASH and Arp2/3 complexes to endosomal membranes. Coordinated activity of WASH and Arp2/3 complexes drives actin polymerization to facilitate endosomal sorting and …
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Structural and biochemical insight into the interactions of Cdc42 with TOCA1 and N-WASP
… on the cytoskeleton via effector proteins of the Wiskott-Aldrich Syndrome (WASP) family and the Transducer of Cdc42-dependent Actin assembly (TOCA) family. The WASP family and their activation by Cdc42 have been thoroughly studied in vitro and are well understood. Conversely, understanding of the …
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Study of T cell activation and migration at the single-cell and single-molecule level
… cells. The same testing procedure was applied to Wiskott-Aldrich syndrome protein (WASp)-deficient T cells that exhibit abnormal morphology and impaired chemotaxis. The stiffness of the diseased cells in the naive state was 1.5 times less than that of the non-diseased cells, a result that may be …
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Sensory Organ Morphogenesis in Caenorhabditis Elegans
… the LIT-1 C-terminus binds both actin and the Wiskott-Aldrich syndrome protein (WASP), an actin regulator. I show that actin also lines the sensory compartment, and that WASP is important for compartment expansion, potentially by functioning in the same pathway as LIT-1. These results suggest …
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Spatiotemporal Control of Actin Cytoskeletal Machinery in Cells: Multivalency Mediated Protein Clustering at the Plasma Membrane and Optogenetic Tool Development
… between Rho-GTPase Cdc42 and its effector Wiskott-Aldrich Syndrome Protein (WASp) to promote filopodia formation in HeLa cells. Using these optogenetic tools, I demonstrate a general method that can be used to study a broad range of proteins and protein-protein interactions.
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Biochemical Reconstitution and Functional Characterization of the WAVE Regulatory Complex
Members of the Wiskott-Aldrich syndrome protein (WASP) family (WASP, N-WASP, WAVE 1-3) have a central role in the transmission of the extracellular signals to the actin cytoskeleton. These proteins use their C-terminal VCA domain to stimulate the actin-nucleating activity of Arp2/3 complex in …
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Investigating the Function of an Actin Nucleation-Promoting Factor N-WASP in Mammalian Myoblast Fusion
Pages 61-89 are misnumbered as pages 75-103, and pages 90-100 are misnumbered as pages 102-112.
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Phase Transitions of Multivalent Adaptor Proteins
Eukaryotic cells efficiently organize their activities to achieve their functional capabilities. This organization of biochemical reactions is a direct result of the cells' ability to compartmentalize their molecules. For example, within a eukaryotic cell, compartments like the nucleus, the …