Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Whole Genome Amplification"”.
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DNA profiling of single sperm cells and single skin flakes in forensics using micromanipulation and whole genome amplification.
… cells and skin flakes. After studying multiple whole genome amplification (WGA) protocols, REPLI-g Single Cell WGA was selected due to its suitability in the pre-amplification step of template DNA. Micromanipulation was used to isolate single spermatozoa. Furthermore, micromanipulation in …
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Short tandem repeat (STR) profile authentication via machine learning techniques
… using standard laboratory techniques such as whole genome amplification and molecular cloning. Such technologies can be used to synthesize any STR profile without the need for a physical sample, only knowledge of the desired genetic sequence. Therefore, to preserve the credibility of DNA as a …
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DEVELOPMENTS TOWARDS SINGLE MOLECULE AND SINGLE CELL GENETIC AND EPIGENETIC ANALYSIS TECHNOLOGIES
… methods and devices for multi- and single cell genome analysis. As the handling of single molecules and single cells are facilitated by microfabricated devices, discussed herein are their designs, fabrication methods, operational procedures, working principles, and experimental results …
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Degenerate oligonucleotide primed amplification of genomic DNA for combinatorial screening libraries and strain enrichment
… When DNA fragments are obtained from a common genome or metagenome and packaged into the same expression vector, this is referred to as a DNA library. Generating quality DNA libraries that incorporate broad genetic diversity is challenging, despite the availability of published protocols. In …
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Virtual microfluidics : a novel single-cell technology based on diffusion-restricted reaction that makes high-quality low-input genomic research accessible
… the smallest unit of life and amplify single genomes for sequencing. Current single-cell technologies require complex instrumentation and the data quality is often confounded by biased genome coverage and chimera artifacts. We address these challenges with a new single-cell technology paradigm …
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Improving genome assembly by identifying reliable sequencing data
De novo Genome assembly and k-mer frequency counting are two of the classical prob- lems of Bioinformatics. k-mer counting helps to identify genomic k-mers from sequenced reads which may then inform read correction or genome assembly. Genome assembly has two major subproblems: contig construction …
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Comparative genomics of chromosomal rearrangements in malaria mosquitoes
… a new protocol for laser microdissection and whole genome amplification of polytene chromosomal fragments to obtain DNA for sequencing and assembly. The chromosomal regions spanning both breakpoints of the 2La in A. arabiensis and A. merus were laser microdissected from the polytene …
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Genetic characteristics of Plasmodium vivax from Northern Mali
… although low parasite density is a challenge for whole genome sequencing (WGS). In the present work, we investigated if selective whole genome amplification (sWGA) can enrich P. vivax DNA extracted from Rapid Diagnostic Tests (RDTs) for Whole Genome Sequencing. We also investigated the origin and …
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Advancing Technologies for Precision Medicine: From Liquid Biopsy to Single-Molecule DNA Sequencing
… protein α (FAPα), were isolated directly from whole blood. Longitudinal monitoring of these CTC subpopulations showed that the ratio of mesenchymal to epithelial CTCs provided an early indicator of treatment response, showing strong agreement with the computed tomography (CT) imaging compared …
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Evaluation and adaptation of molecular approaches for detection and characterization of viruses of the respiratory tract
… the development of novel methods for isothermal amplification of nucleic acids using phi29 and Bst DNA polymerases in the detection and characterization of pathogenic viruses of the respiratory tract. High Resolution Melting (HRM) analysis was used in this study for screening similar sequences to …
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Genetic markers in circulating tumour cells as a measure of the metastatic propensity of uveal melanoma
… to capture CTCs. Secondly, we aimed to evaluate whole genome amplification methods for accurate single cells analysis to determine the genomic profile of UM cells. The combination of both aims would allow the use of UM CTCs for determining disease prognosis from an easily accessible blood sample. …
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Characterisation and evolutionary dynamics of ten novel Gammapapillomavirus types from South African penile swabs
… from 0.5% to 4.1% of men sampled. Multiple full genome clones for each novel type were generated through whole genome amplification, cloning and next generation sequencing. Complete genome sizes were: HPV211 (7253 bp), HPV212 (7208 bp), HPV213 (7096 bp), HPV214 (7357 bp), HPV215 (7186 bp), HPV216 …