Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 114 for “"Western blots"”.
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Maize Transformation for Resistance to Aspergillus Flavus
… integrated into the genome by PCR and Southern blots and the transgene expression detected by RT-PCR, Northern and Western blots, and enzyme activity assays. The ch18 transgene was expressed in embryos as expected and the embryos also had higher chitinase activity than that of the untransformed …
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Nachweis der Expression von Selenoprotein P im menschlichen Gastrointestinaltrakt
… der vorliegenden Arbeit war die Etablierung des Western Blots für den semiquantitativen Nachweis von humanem SeP in Rektumbiopsien. Kernstück der Arbeit war die Anpassung der einzelnen Verfahrensschritte des Western Blots an die besonderen Gegebenheiten bei der Verarbeitung von …
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Gingko biloba extracts protect auditory hair cells from cisplatin-induced ototoxicity by inhibiting perturbation of gap junctional intercellular communication
… (Annexin V/ propidium iodide) and Western blots. The function of GJIC was evaluated by scrape-loading dye transfer (SLDT). Basal turn organ of Corti (oC) explants from neonatal (p3) rats were exposed to cisplatin (1–10μM) and EGb (50–400μg/ml). The number of intact hair cells was …
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Cuticular Proteins of Hyalophora Cecropia: Characterization With Electrophoresis, Antibodies, and Lectins (Insect, Hydrophogicity, Metamorphosis, Glycoproteins, Avidin-Biotin)
… by Periodic Acid-Schiff staining and also on Western Blots of IEF and 2D gels with biotinylated lectins and the avidin-biotin-complex method. More cuticular proteins from flexible cuticular regions than from rigid cuticles were glycosylated, but in both cases these were minor proteins as …
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Aktivierung des nukleären Transkriptionsfaktors kappaB (NF-kappaB) durch Verapamil
… der Zellen wurden gewonnen. Anschließend wurden Western Blots zur Identifizierung von Bestandteilen des Transkriptionsfaktors NF-kappa B und dessen Inhibitorprotein IkappaB durchgeführt. Des weiteren wurden EMSAs zur Aktivitätsbestimmung des Transkriptionsfaktors NF-kappa B durchgeführt. Bei den …
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The Identification and Validation of Novel Aptamers to Glioma
… the PDE1C protein. Whilst validation studies via Western blot showed no aptamer-protein interaction when using the EGFRvIII aptamers, denaturing and native Western blots showed positive results displaying 3 bands indicative of PDE1C isoforms. PDE1C aptamer targets were pulled out of native cell …
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An investigation of the relationship between levels of apoptosis during aging and longetivity
… and caspase activity were examined by way of western blots and caspase assays, respectively. Second, the hypothesis was also tested through the manipulation of DIAP1 expression levels. The number of the UAS-DIAP1 transgene was doubled in the genome, which resulted in an increase in DIAP1 …
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Cellular Mechanism of Arglabin-Dimethylaminohydrochloride Cytotoxicity
… FTI-277 (positive control) for 24 or 72 hours. Western blots and densitometry showed a decreased ratio of processed to unprocessed H-Ras protein in cell lysates incubated with 100 μM of arglabin-DMA. Western blotting of active H-Ras protein from GTPase pull-down assays showed significant …
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In vivo cofactor biosynthesis and maintenance in the class Ia ribonucleotide reductase small subunit of Escherichia coli
… role for interaction with YfaE. Quantitative Western blots of the whole cells suggest that YfaE acts catalytically in reactivating met-[beta]2 in vivo. Titration experiments establish that met-[beta]2 can be reduced by catalytic amounts of YfaE, Fre (a flavin reductase) and flavin with …
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PATHOGENIC ROLE OF PHOSPHODIESTERASE TYPE 5 UPREGULATION IN CARDIAC ISCHEMIA/REPERFUSION INJURY
… protein kinase (PKG) were measured by Western blots and spectrophotometric assay. The results show that ischemia and I/R significantly increased expression of PDE5. H2O2 had no effect on PDE5 expression and activity but significantly increased PKG activity. We conclude that acute …
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Modulation of the Serotonin Reuptake Transporter in RAW264.7 Macrophages
… a time- and concentration-dependent manner and Western blots indicate that the increase in activity is partially due to LPS-induced increases in total SERT protein. Both unstimulated and LPS-stimulated activity was inhibited by the specific SERT inhibitor fluoxetine (IC50= 5-8 nM) and was …
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Identification and Quantification of Protein Carbonylation by Mass Spectrometry
… liquid chromatography/mass spectrometry (LC/MS), Western Blots, and enzymatic assay.
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Regulation of HPr phosphorylation in Mycoplasma pneumoniae
… verschiedenen Wachstumsbedingungen mit nativen Western-Blots untersucht. Die HPrK/P ist nur in Gegenwart von Glycerol als C-Quelle aktiv und phosphoryliert unter diesen Bedingungen ihr Target HPr an der regulatorischen Position Ser-46. Für die Aufklärung der physiologischen Rolle der HPrK/P …
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A putative approach for influencing stomatal density by over-expressing NADP-dependent malic enzyme in arabidopsis
… transgene expression evidence. These included western blots, tissue assay blots, enzyme activity assays, and RT-PCR reactions. Altering NADP-ME expression levels may present a putative approach for influencing stomatal development.</p>
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Translation of Partially Decayed Messenger RNAs in Yeast
… of primer extension, βgalactosidase assay, and western blots, the results indicate that the partially-decayed RNAs are successfully translated, and that addition of xrRNAs results in a 30-50 fold increase in measured enzymatic activity and an accumulation of decay-resistant transcripts in the …
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The isolation of single chain variable region fragments (scFvs) from a phage display library, and expression of the isolated scFvs in Nicotiana benthamiana
… cognate antigens using phage ELISA, phage dot blots and phage western blots. ScFvs displaying the highest affinities for their respective antigens were selected for cloning and expression in plants, as this expression system is scalable, cheaper, safe and facilitates posttranslational …
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Analyse der Funktion und Regulation des ECF-Sigmafaktors YlaC aus Bacillus subtilis
… subtilis-Genoms gefunden werden. Durch Northern-Blots und b-Galaktosidase-Tests mit einem DylaD-Stamm wurde gezeigt, dass YlaD als Anti-Sigmafaktor von YlaC wirkt. Gereinigtes YlaD bindet Zink in äquimolaren Mengen und wird deshalb einer neu-definierten Familie von Zink-bindenden …
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Expression of a tick toxin for the development of a canine vaccine
… to the native toxin in crude tick extract in Western blots. These results indicated that the HT-1 component of the MBP fusion protein was incorrectly folded compared to the native toxin. An expression system using ubiquitin fusion protein was investigated. Ubiquitin is a 10 kDa carrier protein …
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Identification of Actin Binding Proteins Associated with Cross-Linked Actin Networks
… of actin binding proteins in human TM cells. Western blots confirmed expression of actin binding proteins and demonstrated the specificity of selected antibodies. Fluorescence microscopy of treated TM cells showed cytoskeleton rearrangements from linear actin stress fibers to cross-linked …
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Stress response of porphyromonas gingivalis
… of E. coli DnaK and GroEL respectively by Western immunoblotting. On two-dimensional Western blots, two forms of DnaK and four forms of GroEL were identified due to their slightly different isoelectric points. dnaK and groEL gene homologs were identified in several P. gingivalis and some …
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