Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 323 for “"Western blot analysis"”.
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The Dietary Isoprenoid Perillyl Alcohol Inhibits Telomerase Activity in Prostate Cancer Cells
… alcohol or the mTOR inhibitor—rapamycin.</p> <p>Western blot analysis revealed a decrease in hTERT protein levels in response to either agent that did not coincide wholly, with loss of telomerase activity suggesting a further level of regulation. Using immunoprecipitation we established the …
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Content and Activity of Cytochrome P450 3A in Rat Brain Microsomes and Mitochondria
… in the rat brain mitochondria and microsomes by Western blot analysis.</p> <p>Microsome and mitochondria were prepared using different centrifugation methods, followed by purification of the crude mitochondrial fractions using the Percoll density gradient method. The purity of the fractions was …
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Characterization of a Novel Prostate Tumor-Associated Antigen
… (BPH) and carcinoma of the prostate (CaP). Western blot analysis of prostate extracts, showed that MAb TURP-27 and MAb HNK-1 bound glycoproteins of 180, 140, 120, 100, 90 and 69 kDa. Studies have shown that the HNK-1 carbohydrate epitope may be involved in cell adhesion and that it is a …
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caffeine에 의한 HGF-induced G2 delay 촉진효과 확인 및 기전분석
… Same observation was obtained by FACS analysis and mitotic index study. Increased G2 delay by caffeine was also accompanied with the delay in cdk1 activation and in cyclin B nuclear localization. Western blot analysis also showed delayed degradation of cyclin A and B. ATM/ATR knock-down …
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Role of vascular smooth muscle Interleukin-11 in aortic aneurysm development
… of NFAT2, which may promote IL-11 transcription. Western blot analysis was performed to assess IL-11 and NFAT2 levels in VSMCs after exposure to AngII (100 μM), Yoda1 (20 μM), and their combination over 24 hours. IL-11 and NFAT2 expression levels were analyzed using ImageJ. RESULTS: Western blot …
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Production and Characterization of Monoclonal Antibodies against a Recombinant Nfa1 Protein Cloned from Pathogenic Naegleria fowleri
… The McAbs were characterized by isotyping and Western blot analysis. RESULTS: Eight McAbs (A1F, A3C, A6D, A10F, C3A, C6F, C7F, B6B) against the rNfa1 protein were obtained. Eight McAbs isotypes were all IgG2b. The McAbs had high affinity against the recombinant His-tag fusion Nfa1 protein, …
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Characterization of PFF1010c, a type IV Plasmodium fasciparum heat shock protein 40
… under both native and denaturing conditions. Far Western blot analysis to investigate direct interaction between PFF1010c and PfHsp70-1 was conducted and no interaction was observed. Malarial parasites were harvested at different stages and total protein was isolated. The expression of PFF1010c …
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Major Sclerotial Polypeptides Of Psychrophilic Fungi: Identification, Immunological Relatedness, Localization And In Vivo Synthesis
… related by Ouchterlony double-diffusion and Western blot analysis. Polyclonal antibodies used for localizing the major sclerotial polypeptides in the sclerotia by immunofluorescence microscopy showed that the major polypeptides were sequestered in sclerotial protein bodies in all four …
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The expression of Heat Shock Protein 70 in the placental tissue with preeclampsia
… immunostaining, we compared these three groups. Western blot analysis was performed and the quantification and comparison of Hsp70 were done in placental tissue among the three group of patients. RESULTS: All groups demonstrated positive immunostaining for Hsp70 in cytoplasm of placental cells. …
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Studies on the Molecular Mechanisms of Tumorigenesis in Neurofibromatosis Type 1
… N-RAS, H- and K-RAS expression were detected by Western blotting. Particularly, significant up-regulation of K-RAS was detected in the malignant NF1 cells. RAS activation test revealed a large amount of activated form (GTP-RAS) of K- and H-RAS in the malignant NF1 cells. Mutation analysis showed …
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Biochemical and Molecular Approaches to Characterizing the Myrosinase Enzyme System in Horseradish (Armoracia Rusticana)
… gave rise to a band of approximately 66 kDa in western blot analysis. ArM1 has the highest overall amino acid identity (72%) with Arabidopsis thaliana myrosinase TGG2. Phylogenetic analysis shows that ArM1 gene does not cluster with any of the currently known myrosinase subfamily and it may …
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Regulation of Endothelial Nitric Oxide Synthase in Pulmonary Myofibroblasts
… a permeable fluorescein chromophore. By using western blot analysis on fractionated cell lysates we found eNOS expressed in the nucleus under normal growth conditions. eNOS is at least partially regulated by intracellular calcium (Ca2+) and calmodulin (CaM). Western blot analysis using native …
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Vitamin D- induced down regulation of RAD51 in head and neck squamous cell carcinoma (HNSCC), In Vitro and In Vivo
… model. Using immunohistochemical (IHC) and western blot analysis, we demonstrate that systemic application of VD<sub>3</sub>to hamsters downregulates Rad51 expression in the buccal pouch and hinders the onset of tumor formation. Rad51 is a protein that plays a critical role in cell …
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Design and synthesis of aryl hydrocarbon receptor fusion proteins for polyclonal antibodies production and cellular delivery
… significant amount of polyclonal antibodies. In western blot analysis, the antibodies were able to the detect baculovirus expressed AHR and different recombinant proteins of the AHR. The polyclonal antibodies were also used in the gel-shift assay to show the AHR dependent gel shift. Cellular …
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TDI 천식 기도염증반응과 관련된 활성산소의 역할
… oxygen species (ROS) measurement 8 3. Immunoblot analysis 8 4. In vitro cross-linking reaction assay 9 5. Statistical analysis 9 B. Method 10 1. Materials 10 2. Cell culture 10 3. Real-Time polymerase chain reaction 10 4. Preparation of whole, cytosolic and nuclear extracts 11 5. Western blot …
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Differential effects of epidermal growth factor receptor inhibitors on glioblastoma multiforme
… to current treatments. Gene expression analysis has characterized GBM into four main types, with a significant portion belonging to the Classical subtype, typified by overexpression and/or mutation of the epidermal growth factor receptor (EGFR). Also common to this subtype of GBM is the …
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Investigation of the Gracilaria gracilis proteome response to nitrogen limitation
… identities of these enzymes were confirmed by western blot analysis. Additionally, the decreased abundance of fructose 1, 6 biphosphate aldolase during nitrogen limitation observed with two-dimensional gel electrophoresis analysis was validated by enzymatic and western blot analysis. The …
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Physico-chemical characterization of the native and mutant protein cochlin, and its role in adult-onset hearing and balance loss
… liquid chromatography, the Bradford assay, western blot analysis, and proteomics analysis by mass spectroscopy, I acquired data which suggests that the concentration of cochlin in the perilymph of bovine calf ears is at most on the order of 10- mg/ml. I also determined the total protein …
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Characterization of the TCOF1 Gene Using a Neuroblastoma Cell Line and a Mouse Model
… downstream candidate genes using microarray analysis after manipulating Tcofl levels in a murine neuroblastoma (NB) cell line. The list of genes includes cell cycle genes as well as the transcription factors Cnbp and Tbx2, which are known to affect the cell cycle through the c-myc and …
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Divergent Roles of PI3K and Akt in Rapamycin-induced Cardioprotection against Ischemia-Reperfusion Injury
… and 2 hours before harvesting the hearts for Western blot analysis of levels of total or phosphorylated Akt at Ser473. Our results showed that rapamycin protected the heart as observed by a reductionin infarct size from 33.8 ± 2.0% in DMSO-treated hearts to 19.3 ± 4.1% in rapamycin-treated …
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