Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 438 for “"Western Blotting"”.
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Diagnosis of Bovine Anaplasmosis Using Anaplasma Antigenic Markers Identified by Western Blotting
… of using antigenic markers detected by Western blotting (WB) for diagnosis of bovine anaplasmosis was studied.
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Towards Gene Therapy of Parkinson’s Disease: Implantation of RUC-GDNF Secreting Mammalian Cell Lines into Rat Brain
… by the conventional methods such as ELISA and Western blotting, animals must be sacrificed to retrieve implanted cells in order to study their viability and functionality. Real-time monitoring of GDNF production by implanted cells in live animals was not possible.</p> <p>To overcome the limits, …
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The anti-cancer activity of extracts derived from millets and Kraalbos
… epithelial cells. Scratch motility assays and western blotting with antibodies to epithelial to mesenchymal transition markers showed that KB2 inhibited the migratory ability of breast cancer cells. To understand the mechanism(s) underpinning the anti-cancer activity of KB2, ROS-GloTM H2O2 and …
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Molecular analysis of the dry rot fungus Serpula lacrymans
SDS-PAGE/silver staining and western blotting were used to investigate the molecular profile of a PBS soluble extract from standard mycelium profile of S. lacrymans FPRL 12C. Both techniques enabled a reference profile for this organism to be established. A polyclonal antiserum raised against …
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Investigating the potential of the Metastasis Associated Antigen 1 (MTA1) for cancer immunotherapy in a murine model
… MTA1 protein levels were also confirmed by western blotting. It was observed that most of the cell lines expressed MTA1 at high levels, whereas no protein expression was detected in the normal tissues by western blotting. Next, we decided to identify MHC class I and II restricted immunogenic …
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The cardioprotective mechanisms of dietary flavonoids
… and p38 MAPK phosphorylation was monitored using western blotting. It was shown that quercetin was the most potent flavonoid at inducing a protective effect, and 3’-O-methylquercetin the most potent metabolite. Using western blotting it was shown that this protective effect is most likely due to …
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Cytological and Biochemical Studies of Male Sterility in Two Species of Drosophila
… gel electrophoresis, isoelectric focusing and Western blotting to determine the biochemical basis for sterility. One protein, KRP60, was consistently reduced in sterile males. This protein may play a role in the normal function of the sperm in early development.
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The proteomic analysis of resistance to anticancer therapy in human breast cancer
… play a role in resistance to anticancer therapy. Western blotting was used to confirm the expression of selected targets and to ensure the accuracy of protein identification. Examples of those targets which were confirmed by western blotting include GST-M3, cytokeratin 17, peroxiredoxin 4 and …
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Expression and Clinical Significance of the N-myc downstream regulated gene-1 in Hypopharyngeal Cancer
… polymerase chain reaction (RT-PCR) and Western blotting, fresh normal and cancer tissue from the hypopharynx obtained from three patients were used. Results. Positive rates of Ndrg-1 expression in hypopharyngeal SCC were 21.4%, respectively. Ndrg-1 staining was significantly inverse …
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MicroRNA as Regulator of the Th17 Inflammation in Behcet's disease
… CD4+ T cells. Luciferase reporter assay and western blotting were carried out to determine whether Ets-1 is a target of miR-155. Subsequently, Ets-1 expression was quantified using real-time PCR in patients and controls. Western blotting was also performed to investigate the relationship …
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The Possible Role for Basigin Receptor Function in The Induction of Matrix Metalloproteinase Expression in Human Endometrial Stromal Cells
… were screened for the knock-out using PCR and western blotting. I determined that the donor plasmid was inserted into the genomes, but not in the intended location. Upon western blotting, all clones contained basigin protein expression. This could be due to basigin being a key gene in cell …
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Isolation and characterization of bacterial polyhydroxybutyrate inclusions
… inclusions were characterized using 13C NMR, western blotting, SDS-PAGE, atomic force microscopy, and fluorescence microscopy. A model for the structure of native PHB inclusions is proposed.
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Targeting c-Met for therapy
… These purified antibodies were characterised by Western blotting, immunofluorescence staining, functional assays (ERK phosphorylation and cell scatter) and for their ability to recognise native c-Met by flow cytometry. Some of the anti-a-chain c-Met antibodies perform better in Western blotting …
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Evaluation of Anti-cancer effect of a Novel Formulation from Umbilical Cord Blood Stem CellDerived Exosomes and Retinoic acid as a potential therapeutic candidate against Malignant Melanoma
… melanoma cell lines. Moreover, the findings from Western blotting support the RT-qPCR results and the level of upregulation of Caspase-3, P53 and P21 was slightly higher using Western blotting compared to the RT-qPCR and significant initiation of apoptosis was evident in all treatment groups with …
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In vitro studies on genotoxicity and gene expression in spermatogenic cells: mechanisms and assay development
… and qPCR. Protein production was evaluated using Western blotting. Tbpl1, FHL5 and Gtf2a1l were cloned in-frame into the inducible expression vector pET/100-TOPO. The recombinant clones were induced and successful expression of the proteins in E. coli was confirmed by SDS-PAGE and Western …
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Characterization and Regulation of Insulin-Like Growth Factor-Imrna Transcripts in Myeloid Cells
… precursor, was detected in macrophage lysates by Western blotting.
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Investigating alterations in autophagy in Alzheimer’s disease using human brain tissue and skin-derived fibroblasts
… impairment in living patients with AD via Western blotting. The results showed that the autophagy levels decline with increasing Braak, suggesting impairments in both MA and CMA. Also, the results showed that CMA markers were significantly lower in the hippocampus while MA changes were most …
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The effects of chrysin on osteoclastogenesis in RAW264.7 murine macrophages and bone metabolism in Sprague Dawley rats
… on bone from chrysin, a bioactive phytochemical. Western blotting was carried out to assess the impact of chrysin on the RANK signalling pathways. Immunofluorescence was conducted to determine how chrysin effects the translocation of nuclear factor kappa B (NFκB) to the nucleus. Micro-computed …
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Interactions Between Host Cells And Merkel Cell Polyomavirus In The Context Of Infection And In Merkel Cell Carcinoma
… host cell via several methods including qPCR, western blotting, immunofluorescence microscopy, qPCR, and lentiviral CRISPR knockout. We found that MCPyV infection leads to innate inflammatory cytokine induction in response to late events in the viral infectious cycle. This innate gene induction …
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