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Showing 1 to 20 of 875 for “"Western Blot"”.
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Αντισώματα κατά Ro(SSA) και La(SSB) κυτταρικών αντιγόνων: Ανάπτυξη μεθόδου ανοσοαποτύπωσης (Western blot)
An immunoblotting method for the detection of antiRo and antiLa antibodies was developed, using two antigenic sources, thymus extract and human red blood cells. The reactivity of sera from patients with primary Sjogren’s syndrome towards the Ro and La polypeptides was examined and compared with the …
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Separação imunomagnética de cistos teciduais e esporocistos de toxoplasma gondii pelo anticorpo monoclonal K8/15-15 e caracterização de sua proteína alvo por Western Blot e Imunoblot
… sulfate (SDS-PAGE). The antigen was processed by Western blot, and bands of 272 and 381 kDa were labeled by IMUNOBLOT. The same bands were observed in a separate SDS-PAGE stained by Comassie blue. The results obtained here will favor the conduction of further experiments to fully characterize the …
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Einfluss der Hypothermie auf die Expression der Apoptose-assoziierten Proteine nach inkompletter cerebraler Hemisphären-Ischämie bei der Ratte über einen Beobachtungszeitraum von 28 Tagen
… anschließend in Serie geschnitten oder für die Western-Blot-Analyse aufbereitet. Die Nativtiere tötet man ohne jegliche Manipulation unter Narkose, um für die anschließenden Analyseverfahren einen physiologischen Ausgangswert zu etablieren. Die Expression der Apoptose-assoziierten Proteine Bax, …
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Prokaryotic Production of Human Immunodeficiency VirusType 1 Subtype C Tat, Nef and Reverse Transcriptase andInvestigation of Antibody Responses to these proteins inHIV-1 Infected Individuals as well as Macaques Vaccinatedwith SAAVI DNA-C/C2 and SAAV
… in any of the 20 negative control sera in the Western blot assay. Analysis of 481 sera from HIV-1 infected individuals showed that the majority of serum samples had relatively high prevalence of anti-RT antibodies, ranging from 89.9% to 95% irrespective of the clinical stages. Anti-Nef …
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DOCKING STUDIES OF CURCUMIN ANALOGS AGAINST METHIONINE AMINOPETIDASE 2 AND BIOLOGICAL EVALUATION OF LEAD MOLECULES IN COLON CELLS
… MetAP2 expression in the HT29 cell line using Western blot analysis. The Student’s t-test for paired data and Dunnett’s post hoc test with p<0.05 were performed using the program IBM SPSS Statistics 26. One hundred thirty compounds were docked against MetAP2 enzyme, out of these the top 10 …
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Binding mechanism of K88ab pili produced by enterotoxigenic Escherichia coli
… was characterized by inhibition assay and Western blot. In Western blot, K88ab pili were bound by two major brush border membrane polypeptides with molecular weight of 61,500 and 57,000 in addition to numerous minor polypeptides and a major mucus polypeptide with molecular weight of 27,500. …
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A flotillin-1 és vimentin fehérjék kölcsönhatásának vizsgálata a PP2A Bα fehérjével endotél sejtekben
… A fehérjéket termeltettük, majd pull down és Western blot kísérletekkel megvizsgáltuk. A kísérletek eredményét részletesen taglaltam, képekkel mellékelve.
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Characterization of PFF1010c, a type IV Plasmodium fasciparum heat shock protein 40
… under both native and denaturing conditions. Far Western blot analysis to investigate direct interaction between PFF1010c and PfHsp70-1 was conducted and no interaction was observed. Malarial parasites were harvested at different stages and total protein was isolated. The expression of PFF1010c …
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Immunoreactivity of anti pZP antibodies from the serum of SpayVac vaccinated mares to equine zona protein
… vaccinated mares to equine zona protein using Western blot and immunohistochemical techniques. These techniques were first applied using a bovine model because bovine oocytes are more easily obtained in large quantities relative to equine oocytes. Once the procedure was validated, equine …
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The role of copper in Parkinson's disease
… coupled plasma-mass spectrometry (ICP-MS), western blot and immunohistochemistry demonstrated the substantia nigra contains twice as much copper compared with other brain regions, suggesting an important role for copper in this region. Synchrotron-based X-ray fluorescence microscopy, …
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The Dietary Isoprenoid Perillyl Alcohol Inhibits Telomerase Activity in Prostate Cancer Cells
… alcohol or the mTOR inhibitor—rapamycin.</p> <p>Western blot analysis revealed a decrease in hTERT protein levels in response to either agent that did not coincide wholly, with loss of telomerase activity suggesting a further level of regulation. Using immunoprecipitation we established the …
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Immunogenität translozierter Effektorproteine bei Infektionen mit Bartonella henselae
… Versuche, einen sensitiven und spezifischen Western Blot zur Diagnostik einer B. henselae-Infektion aufzubauen, scheiterten an der mangelnden Immundominanz der eingesetzten Antigene. In der vorliegenden Arbeit wurden acht rekombinante Proteine von B. henselae auf ihre Immundominanz im Western …
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Content and Activity of Cytochrome P450 3A in Rat Brain Microsomes and Mitochondria
… in the rat brain mitochondria and microsomes by Western blot analysis.</p> <p>Microsome and mitochondria were prepared using different centrifugation methods, followed by purification of the crude mitochondrial fractions using the Percoll density gradient method. The purity of the fractions was …
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Expression of Epidermal Growth Factor Receptor in hypopharyngeal cancer and Effect of EGF in Progression of hypopharyngeal cancer
… and hypopharyngeal SCC with EGFR antibody. For Western blot, fresh normal and cancer tissue from the hypopharynx obtained from three patients were used. We performed RT-PCR and Western blot analyses of FaDu cells. Proliferative of the FaDu cells was assayed by counting the number of the cells …
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Production of Extracellular Matrix-Degrading Proteases by a Rat B Cell Line.CRL-1631
… and uPAR protein expression was confirmed by Western blot analyses. I also report, for the first time, MT -1 MMP gene and protein expression in B cells by RT-PCR and Western blot, respectively. CRL-1631 invasion through Matrigel model basement membrane was significantly inhibited by BB-94, …
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Characterization of a Novel Prostate Tumor-Associated Antigen
… (BPH) and carcinoma of the prostate (CaP). Western blot analysis of prostate extracts, showed that MAb TURP-27 and MAb HNK-1 bound glycoproteins of 180, 140, 120, 100, 90 and 69 kDa. Studies have shown that the HNK-1 carbohydrate epitope may be involved in cell adhesion and that it is a …
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Characterizing meat tenderness of finishing steers fed for different rates of gain
… Individual grade and yield data were collected. Western blot samples and WBSF steaks were collected 24 hrs postmortem from the strip loin and randomly assigned to an aging time point (1, 7, 14, 21, 28, or 35 d). Western blot samples and shear force steaks were vacuum packaged and stored at 4 °C …
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A takarmánykorlátozás befolyásolja a házityúk inzulinreceptorának expresszióját
… PCR (qPCR), míg a fehérje szintű vizsgálatok western blot segítségével történtek.
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Identification of Neuropeptide FF receptor 2 (NPFFR2) and its ligand NPFF as a factor to affect cancer progression
… 분리 및 RT-PCR 5 C. 발현 조절 및 펩티드를 이용한 수용체 활성화 6 D. Western blot 6 E. Invasion assay 7 F. Wound healing assay 7 G. Colony formation assay 8 Ⅲ. 결과 9 Ⅳ. 고찰 26 Ⅴ. 결론 30 참고문헌 31 ABSTRACT 38
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Evaluation of nanoscale delivery of miR 216 a/b, miR 217, and gemcitabine in pancreatic cancer cell lines
… determine the underlying molecular mechanisms, Western blot analysis was conducted on lysates and supernatants from treated cells. Target proteins related to apoptosis, epithelial-to-mesenchymal transition, and oncogenic signaling pathways were evaluated. While some expected trends were …
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