Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Wee1"”.
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DNA stress checkpoint control in Arabidopsis thaliana by WEE1
The WEE1 kinase is a conserved cell cycle inhibitor that, together with CDC25, controls CDK activity and consequently the G2/M progression in yeast and animals. The same control mechanism is utilized by the DNA damage checkpoint to induce a cell cycle arrest upon DNA stress. In plants, a homolog of …
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THERAPEUTIC POTENTIAL OF TARGETING THE FLNA-REGULATED WEE1 KINASE PATHWAY IN ADRENOCORTICAL CARCINOMAS.
… 1 receptor (IGF1R) signalling. Upregulation of Wee1 nuclear kinase, a key regulator of G2/M cell cycle checkpoint, was shown in mouse neural progenitor cells lacking FLNA, and it has been reported in several cancers. The first part of this PhD thesis is focused 1) on the analysis of Wee1 …
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The Role of Chk2 and Wee1 Protein Kinases during the Early Embryonic Development of Xenopus laevis
… the roles of two protein kinases, Chk2/Cds1 and Wee1, during checkpoint signaling in the developing embryo. The DNA damage checkpoint is mediated by the Chk2/Cds1 kinase. Conflicting evidence implicates Chk2 as an inhibitor or promoter of apoptosis. To better understand the developmental function …
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Mechanisms of cell cycle remodeling at the MBT during the development of Xenopus laevis embryos
… E and onset of the MBT. GST-D34Xic1 lowers Wee1 level, a kinase that maintains Cdks in an inactivate state. Eventual degradation of cyclin E is preceded by degradation of GST-D34Xic1. The mathematical modelers, Andrea Ciliberto and John Tyson, incorporated the data into a kinetic model and …
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Characterisation of the molecular complexes that regulate the G2/M checkpoint of the eukaryotic cell cycle
… B1 complex; phosphorylation of CDK1 by wee1 kinase prevents progression to mitosis, and subsequent dephosphorylation by the CDC25 phosphatases, initially the B isoform, leads to mitotic onset. The aim of this research was the biophysical and/or biochemical characterisation of the …
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THE INHIBITOR-OF-APOPTOSIS PROTEIN SURVIVIN INCREASES P34CDC2 PHOSPHORYLATION AND ENHANCES CELL SURVIVAL AND PROLIFERATION BY PROTECTING THE WEE1 KINASE FROM DEGRADATION BY CASPASE-3
… wt-Survivin, 2-fold higher levels of Wee1 protein were observed compared to cells expressing control vector alone. Treatment of control BaF3 cells with the caspase-3 inhibitor Ac-DEVD-CHO increased both Cdc2-Tyr15 phosphorylation and Wee1 protein levels. In a similar fashion …
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Pyp3 Involvement in Mitotic Control and Cell Growth in Fission Yeast
… in the regulation of the cell cycle. The kinases wee1+, mik1+ and the phosphatases cdc25+, pyp3+ play opposite roles in the regulation of p34cdc2 activity. In cells lacking wee1, tyrosine phosphorylation of p34cdc2 is carried out by Mik1; in cells lacking cdc25+, the activity of the Pyp3 …
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A model of the checkpoint response of the cell cycle of frog-egg extracts in the presence of unreplicated DNA
… (MPF), and its key regulatory proteins Cdc25 and Wee1, are central parts of this cell cycle engine. Maintaining the fidelity of the DNA during the cell cycle is critical for successful propagation of the cell lineage. In the presence of unreplicated DNA, the cell cycle engine''s progress into …
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Identification Of Effective New Drugs Combinations Exploiting The Ability Of trabectedin To Modulate Transcription
… on the combination of trabectedin and the WEE1 inhibitor, AZD-1775, have shown that the inhibition of WEE1 enhances the trabectedin activity, thus the combination is synergic. Since trabectedin affects the transcription of several genes, I have developed a new approach based on the use of …
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Mathematical Modeling Reveals That G2/M Checkpoint Override
… TSC2-null cells were more sensitive to the WEE1 inhibitor, a negative regulator of mitotic entry, compared to wild-type cells.</p> <p><strong> In summary</strong>, we reported a novel mechanism of the mTORC1 signaling in regulating a transcriptional program required for G2/M checkpoint …
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Mathematical Modeling Reveals That G2/M Checkpoint Override Creates A Therapeutic Vulnerability In Tsc2-Null Tumors
… TSC2-null cells were more sensitive to the WEE1 inhibitor, a negative regulator of mitotic entry, compared to wild-type cells.</p> <p><strong> In summary</strong>, we reported a novel mechanism of the mTORC1 signaling in regulating a transcriptional program required for G2/M checkpoint …
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Mathematical Modeling Reveals That G2/M Checkpoint Override Creates A Therapeutic Vulnerability In Tsc2-Null Tumors
… TSC2-null cells were more sensitive to the WEE1 inhibitor, a negative regulator of mitotic entry, compared to wild-type cells.</p> <p><strong>In summary</strong>, we reported a novel mechanism of the mTORC1 signaling in regulating a transcriptional program required for G2/M checkpoint …
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Deciphering The Role of miR-506 in Ovarian Cancer Drug Sensitivity
… (DDR) pathway, like RAD51, RAD17, CHEK1 and WEE1 and I concentrated on genes not previously studied in EOC. I validated RAD17 as a direct target of miR-506 and identified the miR-506-RAD17 axis as relevant in chemosensitising EOC cells to different treatments. I demonstrated that miR-506 …
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Rational identification of a dual inhibitor of PARP-1 and ATM kinase
… 1/2), DNA-PK (DNA-dependent protein kinase) and WEE1 (WEE1 G2 Checkpoint Kinase) proteins. These compounds are being developed for use in patients with specific genetic markers or as combination therapies with chemotherapy or radiation. PARP inhibitor monotherapy treatment can lead to resistance …
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Regulation of the FGF14:Nav Interactome by a GSK-3 Centered Pathway
… focused GSK-3 interactome, comprised of Akt, Wee1, and CK2, disrupts Nav complex assembly, changes the phosphorylation status of the FGF14 N-terminus, alters the sub-cellular localization and expression of Nav complex proteins, and affects neuronal firing. These findings suggest that FGF14 may …
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Controls Over S-Phase And Over Nuclear Synchrony
… that inhibition of CDK activity by Rum1 and Wee1 may coordinate and synchronize these cell cycle events in the common cytoplasm.</p>
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Part 1 Design, Synthesis and Bioactivity of a Phosphorylated Prodrug for the Inhibition of Pin1; Part 2 Conformational Specificity of Cdc25c Substrate for Cdc2 Kinase using LC-MS/MS
… phosphatase,Cdc27, p53 oncogene, c-Myc oncogene, Wee1 kinase, Myt1 kinase, and NIMA kinas, have been confirmed as substrates of Pin1. Pin1 was also observed to be overexpressed in a variety of cancer cell lines, and the inhibitors of Pin1 showed antiproliferative activities towards these cancer …
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Design, Syntheses, and Bioactivities of Conformationally Locked Pin1 Ground State Inhibitors
… enzymes include Cdc25, Cdc27, Cyclin D1, Myt1, Wee1, NIMA, Cdc2, Plk1 and c-Myc. Recent research has revealed that Pin1 is overexpressed in a variety of cancer cell lines and Pin1 inhibitors inhibit proliferation activity of several cancer cells overexpressing Pin1. The most potent Pin1 …
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Identifying Mechanisms Of Resistance And Potential Therapeutic Targets For Pediatric Acute Myeloid Leukemia
… AZD1152-HQPA, the Plk1 inhibitor BI6237, or the wee1 inhibitor MK-1775. It was found that MK-1775, in contrast to the other three agents, synergized with araC in antiproliferative MTT assays in both cell lines as well as in ex vivo DS-AML primary patient samples. MK-1775 was able to decrease …
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Mitotic slippage by ATP depletion and its mechanism
… mitotic slippage by treatment with PD166285, a Wee1/Myt1 inhibitor, partially reduced the induction of mitotic slippage by ATP depletion. Induction of mitotic slippage by direct inhibition of Cdk1 kinase activity using RO3306 treatment was also observed the possible involvement of Cdk1 …