Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 20 for “"WW domain"”.
-
Pin1 Catalytic and WW Domain Ligands
… prolyl isomerase (PPIase) enzyme with two domains, the catalytic domain and the WW domain. Both domains specifically bind pSer/pThr–Pro motifs. Pin1 plays an important role in regulating the cell cycle, and it is involved in many diseases, such as cancer, HIV-1, Alzheimer's disease, asthma, …
-
Pin1: WW domain ligands, catalytic inhibitors, and the mechanism
… Binding Assay (ELEBA) for the identification of WW domain ligands, (2) a catalytic assay to identified inhibitors of Pin1 catalytic activity. The ELEBA offers a selective approach for detecting ligands that bind to the Pin1 WW domain from chemical libraries. By using the ELEBA, a pSer-Pro …
-
A Survey of Beta-Sheet Folding: WW Domains With Zinc Fingers and Membrane Peptides
… fast-folding protein systems. By perturbing a WW domain sequence with side-chain mutations, truncations, amide-to-ester exchanges, and loop rearrangements we find that loop structures can dictate the folding dynamics of this model beta-sheet protein. We then report on a synthetic zinc-finger …
-
Evolutionary Constraints Specifying Protein Folding and Function
… design constraints to allow the design of novel WW domain sequences that fold and function like natural proteins. This work expands the initial investigations, and probes the minimal information content necessary to specify the WW domain fold, as well as the effect of increasing the coupling …
-
Exploring Downhill Protein Folding Free Energy Landscapes
… A kinetic and thermodynamic survey of multiple WW domain mutants is performed in combination with a model to reveal the correlation between protein stability and downhill folding propensity. A designed histidine containing lambda repressor mutant is identified to be a downhill folder even at its …
-
Decoding the structure WWP2 in the TGFβ signalling pathway
… system lies in the protein-protein interaction domains of the diverse group of E3 enzymes. WWP2 is one such E3 enzyme, and the relevant protein-protein interaction domain is the WW domain. WWP2 has four WW domains which are used to interact with proline-rich motifs found in the sequences of the …
-
In vitro and in vivo protein folding under stress
… conditions. In chapters 2 and 3, fast-folding WW domains were studied (best-characterized systems for comparing experiments with simulations) by T-jump relaxation in conjunction with protein engineering. Chapter 1 is a comprehensive data set of mutational Φ-values (ΦM) as indicators for folding …
-
Novel methods in computational analysis and design of protein-protein interactions : applications to phosphoregulated interactions
… of phosphopeptides with phosphopeptide-binding domain interactions. A novel protein-protein interaction type, the action-at-a-distance interaction, is described in the complex of the TEM1 P-lactamase with the 3-lactamase inhibitor protein (BLIP). New action-at-a-distance interactions were …
-
Negative biomarkers of sperm quality and male fertility
… structure assay (SCSA) and the post-acrosomal ww-domain binding protein (PAWP) to human assisted reproductive therapy and an agricultural bull stud, respectively. Analysis of the results of SCSA, infertility treatment outcomes, and the occurrence of spontaneous abortions (SAB) and multiple …
-
Application of All-Atom and Coarse-Grained Molecular Dynamics Simulations to Long Timescale Structural Transitions of Proteins
… plant phototropins, the folding process of the WW domain and villin headpiece, the assembly and stability of a variety of viral capsids, the assembly and disassembly of high density lipoprotein particles, and the rotation of the bacterial flagellum. In each case, molecular dynamics simulations …
-
Protein and RNA folding: from bulk towards high throughput single molecule experiments
… long MD simulations of a variant of the β-sheet WW domain protein Fip35, were experimentally verified to be true. This provided an atomistic detail understanding of the folding landscape and will act as a key benchmark in unraveling the protein folding problem. Thirdly, protein and RNA …
-
Frustration of protein folding from in vitro to in vivo
… explores the folding of a fast-folding protein (WW domain variant) in a cell-like environment. Chapter 5 reports work on protein recognition by the proteasome, where we investigated protein waste recycling in cells and found that protein re-folding is a vital process in 26S proteasome to initiate …
-
Investigating Protein Folding Pathways at Atomistic Resolution: from a Small Domain to a Knotted Protein
… studying the folding pathways of the Fip35 WW Domain, a 35 amino-acids long protein. Performing all atom simulations we were able to show that this small protein folds following only two pathways, defined by the order of formation of secondary structures. Notably, our results are compatible …
-
The genetic and biochemical analysis of Drosophila Wwox protein function.
WWOX (WW domain-containing oxidoreductase) is a candidate tumor suppressor gene that has been shown to be involved in various cancers including breast, lung, prostate, gastric and hepatic. The Drosophila ortholog Wwox was identified and subjected to targeted ‘loss of function’ mutagenesis. The …
-
Studies of the Hippo Signaling Pathway
… proteins, the Ste20-like kinase Hpo, the WW domain-containing protein Salvador (Sav), the NDR family kinase Warts (Wts) and the Mob family protein Mats. The kinase activities of Hpo and Wts are facilitated by their regulatory proteins Sav and Mats, respectively. Activated Hpo/Sav complex …
-
Regulation of RUNX3 in gastric and breast cancer
… recognition of the PY motif of RUNX3 by a WW domain of CagA. Deletion of the WW domains of CagA or mutation of the PY motif in RUNX3 abolishes the ability of CagA to induce the ubiquitination and degradation of RUNX3, thereby extinguishing its ability to inhibit the transcriptional …
-
Phase Phenomena of Proteins in Living Matter
… examine the interplay between folding and inter-domain interactions of engineered FiP35 WW domain repeat proteins with $n = 1$ through 5 repeats using a coarse-grained simulated annealing with the AWSEM Hamiltonian. According to our simulations, misfolded structures become increasingly prevalent …
-
Characterizing Multivalent Interactions Between Folded Protein Domains and Intrinsically Disordered Regions or Peptide Substrates
… with a focus on interactions between structured domains and intrinsically disordered regions or peptide substrates.The subject of Chapter two is prolyl isomerase Ess1, which is an essential enzyme found in Saccharomyces cerevisiae. Ess1 regulates the transcription and co-transcriptional RNA …
-
Protein folding in living cells and under pressure
… refolding kinetics of the engineered WW domain FiP35, a model system for beta sheet folding. With a full complement of molecular dynamics experiments mimicking experimental conditions, we show that simulation and experiment are consistent with a four-state kinetic mechanism and …
-
Characterising the effects of ACK-mediated phosphorylation on the Class IA PI3-Kinase regulatory isoforms
… androgen receptor, the oestrogen receptor and WW domain containing oxidoreductase. Despite enormous progress in recent years, much remains to be learnt concerning ACK's role in oncogenic signalling. To gain further insight, the Owen lab conducted a yeast two-hybrid screen to identify novel ACK …