Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"WRN"”.
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Biochemical and Structural Insights into VCP Unfoldase and WRN Helicase Complexes
… the biochemical and structural properties of WRN helicase in complex with DNA substrates. I describe multiple efforts toward structure determination of WRN-DNA assemblies and additional studies where I examined the mechanism of action for a clinical-stage small molecule WRN inhibitor. While I …
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THE FUNCTION OF MRN (MRE11-RAD50-NBS1) COMPLEX DURING WRN (WERNER) FACILITATED ATM (ATAXIA-TELANGIECTASIA MUTATED) ACTIVATION
WRN (Werner) protein is a member of the RecQ family showing helicase and exonuclease activity. WRN protein may lose function upon mutation and causes Werner syndrome (WS) which is an autosomal recessive, cancer-prone and premature aging disease. ATM (Ataxia-Telangiectasia mutated) protein initiates …
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Investigating the regulation of DNA non-homologous end-joining through Ku70/80 interacting factors
… I investigate the role of the RecQ helicase WRN, whose precise roles in the DDR are unclear. As an interactor of both HR and NHEJ proteins, WRN may affect the regulation of both pathways. WRN knockout cells were generated and a CRISPR-Cas9 screen performed to identify suppressors of WRN …
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Effect of Werner and Bloom Helicase Deficiencies On Dna Double-Strand Break Repair
… help maintain genomic stability and integrity. WRN and BLM are helicases in the RecQ helicase family. Mutations in the genes for each helicase result in a severe genetic disease. Werner syndrome is caused by a mutation in the WRN gene. Patients with this disease show signs of premature aging and …
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Functions of Human DNA2 and its Protein Partners in DNA End Resection
… for our assays. Further I produced DNA2 partners WRN and BLM helicases and various single-stranded DNA binding proteins such as the replication protein A (RPA). In my experiments, I could recapitulate DNA2’s nuclease activity and its regulation by RPA. While in the absence of RPA DNA2 degrades 3’ …
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Harnessing CRISPR-Cas9 screens to identify functional genetic interactions
… that can drive resistance or hypersensitivity to WRN-depletion. This work amplifies the evidence for WRN-depletion as a promising new avenue for chemotherapy in cancer models with MSI. Altogether, this work demonstrates the potential of CRISPR-Cas9 screening technologies in identifying functional …
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DNA repair and sister chromatid exchange
… the role of HR by investigating the Werner (WRN), Bloom (BLM), and FANCD2 proteins. I also investigated the role of non-homologous end joining (NHEJ) by examining the DNA-dependent protein kinase (DNA-PKcs), both the Ku70/80 heterodimer and the catalytic subunit (DNA-PKcs), and Artemis. ERCC1 …