Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Vitelline Membrane"”.
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Investigation of the role of VML in the establishment of Drosophila embryonic dorsal-ventral polarity
… groups to several protein components of the vitelline membrane layer of the eggshell, including Vitelline Membrane-Like protein (VML). These sulfated proteins represent a ventral cue embedded in the eggshell, which, during embryogenesis, leads to the spatially-restricted activation of a …
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An Investigation of the Genesis of Lumbosacral Myeloschisis in the Chick Embryo
… had been induced by the removal of the vitelline membrane over the blastoderm at the 13-, 14-, 15- or 16-somite stages. Defective embryos were examined histologically at 3-hour intervals from the time of treatment to 30 hours of reincubation and thereafter at 12-hour intervals until 66 …
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Vasculogenic Scaffolds: How Cell-Cell and Cell-Matrix Interactions Regulate Vascular Differentiation and Morphogenesis
… available collagen cell carrier (CCC) and the vitelline membrane (VM) from a chick egg. Two cell types with vasculogenic potential were used in this study, human umbilical vein endothelial cells (HUVECs) and rat bone marrow stem cells (BMSCs). The vaculogenesis as well as levels of SMαA and vWF …
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Investigating the calcium wave and actin dynamics at Drosophila egg activation
… of maternal mRNAs and cross-linking of the vitelline membrane. While some aspects of egg activation, such as initiation factors in mammals and environmental cues in sea animals, have been well-documented, the mechanics of egg activation in many animals are still not well understood. This is …
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The oocyte-to-embryo transition : regulation of oocyte maturation and egg activation in Drosophila
… in DNA replication, ROS/RNS redox pathways, and vitelline membrane crosslinking are explored. Furthermore, current research into the role of Ca²+ as a regulator of Drosophila egg activation is summarized. Recent studies have suggested that Sarah (Sra), a regulator of Calcineurin (CN), is required …
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Nicarbazin for contraception of Canada geese: laboratory and field studies
… mg/kg body weight group. Degeneration of the vitelline membrane was evident at all treatment levels, with severity being dose-related and greater in the outer vitelline membrane than the inner vitelline membrane. No significant health effects were observed for birds treated with NCZ. The …
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Studies on Fertilization in Vitro in Mammals
… the sperm appeared to be embedded into the egg vitelline membrane however total incorporation of the sperm into the egg was not observed. Mouse thymocytes underwent premature chromosome condensation when artificially fused to unactivated mouse eggs. When sperm and thymocytes were fused to …
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Analysis of metazoan DNA replication initiation using Drosophila gene amplification as a model system
… cells. Vm34Ca is a structural component of the vitelline membrane but is expressed prior to the onset of gene amplification. CG16956 is expressed in amplification stages but only in a small subset of follicle cells. Like the previously characterized DAFC-62D, DAFC-34B displays origin firing at …
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Mechanical mapping of lineage boundaries in the developing Xenopus laevis embryo
… First, I determined that the presence of the vitelline membrane obscures the mechanical properties of the embryo below, and so must be removed, and that the AFM could reliably measure embryonic tissue up to an angle of 21° from horizontal. I then measured the stiffness and viscosity of three …
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Modeling human Usher syndrome during Drosophila melanogaster development
… microvillar protrusions of the apical membrane of inner ear hair cells, are organized into coherent bundles. This precise organization is critical for mechanosensing, i.e. for hearing. Mutation in any of the five known Usher syndrome genes is sufficient to alter the precise organization …
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Calcium and actin coordinate egg activation and the metaphase-anaphase transition in Drosophila oocytes
… that calcium enters the oocyte from the peri- vitelline space (between the oolemma and vitelline membrane) through Trpm in the form of a wave. I next ask what mechanisms regulate calcium entry through Trpm channels. I provide detailed visualisation of the actin population in the mature oocyte …
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Optimising light-sheet microscopy for high resolution live imaging of the early development of chick embryos
… and a labelled structure such as the cell membrane, simultaneously.<br/><br/>Further changes on the microscope were implemented in the camera operation included the use of confocal line detection, which greatly reduced background signals and increased image contrast. This improvement …