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Showing 1 to 20 of 20 for “"Vitelline"”.
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The influence of blood flow on angiogenesis in the 3-day chick embryo
… of the anterior cardinal vein, anterior vitelline vein, vitelline artery, and vitelline vein were not statistically different from sham and control values. Eye diameter increased equally in the presence and absence of blood flow. These results demonstrate that blood flow had no influence …
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Investigation of the role of VML in the establishment of Drosophila embryonic dorsal-ventral polarity
… groups to several protein components of the vitelline membrane layer of the eggshell, including Vitelline Membrane-Like protein (VML). These sulfated proteins represent a ventral cue embedded in the eggshell, which, during embryogenesis, leads to the spatially-restricted activation of a …
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An Investigation of the Genesis of Lumbosacral Myeloschisis in the Chick Embryo
… had been induced by the removal of the vitelline membrane over the blastoderm at the 13-, 14-, 15- or 16-somite stages. Defective embryos were examined histologically at 3-hour intervals from the time of treatment to 30 hours of reincubation and thereafter at 12-hour intervals until 66 …
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Post-translational modifications of SEL24K from salmon eggs and ZPA from Xenopus laevis eggs
… the glycosylation of a glycoprotein ZPA from the vitelline envelope of Xenopus laevis was determined by applying a strategy of general proteolysis coupled with mass spectrometry. The vitelline envelope glycoproteins were first separated through SDS-PAGE. A nonspecific in-gel pronase digestion was …
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Vasculogenic Scaffolds: How Cell-Cell and Cell-Matrix Interactions Regulate Vascular Differentiation and Morphogenesis
… available collagen cell carrier (CCC) and the vitelline membrane (VM) from a chick egg. Two cell types with vasculogenic potential were used in this study, human umbilical vein endothelial cells (HUVECs) and rat bone marrow stem cells (BMSCs). The vaculogenesis as well as levels of SMαA and vWF …
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An ultrastructural study of gametogenesis and early development in the sea anemone <i>Actinia fragecea</i> (Cnidaria : Anthozoa)
… three occasions. Spawned eggs do not possess a vitelline coat, and do not undergo a cortical reaction. Gastrulae may take up numerous supernumary sperm.
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Novel Approaches to Thalassaemia Gene Therapy
… utero delivery model system. Injection via the vitelline vessel at E14 and El6 showed little difference with respect to marking of erythroid precursors. However, there appeared to be a higher level of transduced myeloid cells from injections conducted at El6. Following this, we performed …
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Calcium and actin coordinate egg activation and the metaphase-anaphase transition in Drosophila oocytes
… that calcium enters the oocyte from the peri- vitelline space (between the oolemma and vitelline membrane) through Trpm in the form of a wave. I next ask what mechanisms regulate calcium entry through Trpm channels. I provide detailed visualisation of the actin population in the mature oocyte …
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Comportamento reprodutivo de Mussismilia hispida (Verril, 1902) (Cnidaria, Anthozoa, Scleractinia)
… which characteristically had a large amount of vitelline material and a peripheral germ vesicle.
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The Effects of Salinity on Zootechnical Performance of Spotted Seatrout (<i>Cynoscion nebulosus</i>) in Recirculating Aquaculture Systems
… post-hatch did not differ between treatments. Vitelline reserves were utilized more slowly in the lower salinity treatment groups than in the high salinity treatment.</p> <p>Juveniles from two age groups (42 and 210 days old) were cultured in parallel for 267 days at four salinities (10, 15, …
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Investigating the calcium wave and actin dynamics at Drosophila egg activation
… of maternal mRNAs and cross-linking of the vitelline membrane. While some aspects of egg activation, such as initiation factors in mammals and environmental cues in sea animals, have been well-documented, the mechanics of egg activation in many animals are still not well understood. This is …
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The oocyte-to-embryo transition : regulation of oocyte maturation and egg activation in Drosophila
… in DNA replication, ROS/RNS redox pathways, and vitelline membrane crosslinking are explored. Furthermore, current research into the role of Ca²+ as a regulator of Drosophila egg activation is summarized. Recent studies have suggested that Sarah (Sra), a regulator of Calcineurin (CN), is required …
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Nicarbazin for contraception of Canada geese: laboratory and field studies
… mg/kg body weight group. Degeneration of the vitelline membrane was evident at all treatment levels, with severity being dose-related and greater in the outer vitelline membrane than the inner vitelline membrane. No significant health effects were observed for birds treated with NCZ. The …
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Studies on Fertilization in Vitro in Mammals
… the sperm appeared to be embedded into the egg vitelline membrane however total incorporation of the sperm into the egg was not observed. Mouse thymocytes underwent premature chromosome condensation when artificially fused to unactivated mouse eggs. When sperm and thymocytes were fused to …
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Analysis of metazoan DNA replication initiation using Drosophila gene amplification as a model system
… cells. Vm34Ca is a structural component of the vitelline membrane but is expressed prior to the onset of gene amplification. CG16956 is expressed in amplification stages but only in a small subset of follicle cells. Like the previously characterized DAFC-62D, DAFC-34B displays origin firing at …
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The Effects of Toluene on the Ontogenetic Development of the Fish, Oryzias latipes, the Japanese Medaka
… blastomeres, (2) malformation of the heart and vitelline circulatory systems, (3) deformation of the eyes, (4) malformation of the tail musculature as indicated by a bending of the tail, and (5) a failure of some of the visceral organs to develop properly. Tissue of a mesodermal origin in whole …
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Mechanical mapping of lineage boundaries in the developing Xenopus laevis embryo
… First, I determined that the presence of the vitelline membrane obscures the mechanical properties of the embryo below, and so must be removed, and that the AFM could reliably measure embryonic tissue up to an angle of 21° from horizontal. I then measured the stiffness and viscosity of three …
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The transfer of antibody from mother to foetus in the guinea-pig
… to be via the yolksac splanchnopleur and the vitelline circulation of the foetus (Barnes, 1957). The present work was undertaken to investigate by the technique of intra-uterine injections of antitoxic sera used by Brambell and colleagues the selectivity of the yolk-sac splanchnopleur of the …
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Modeling human Usher syndrome during Drosophila melanogaster development
… associates with defective deposition of the vitelline membrane, an extracellular matrix that protects the embryo from osmotic stresses. These findings suggest that microvilli are normally required for the even deposition of the extracellular matrix. In order to test whether Cad99C is involved …
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Optimising light-sheet microscopy for high resolution live imaging of the early development of chick embryos
… epiblast in the space between the embryo and the vitelline membrane. These vesicles were especially prominent in the region where the mesendoderm precursor cells ingress. Confocal line detection allowed closer observation of vesicles formation process, suggesting that the vesicles were released …