Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Virus growth"”.
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HEPATITIS A VIRUS: GROWTH CHARACTERISTICS, PURIFICATION, AND CAPSID GENE ORDER (PEPTIDES, IMMUNOREACTIVITIES, POLYPEPTIDES).
A human isolate of hepatitis A virus (HAV) strain HAS-15 was adapted to rapid growth FRhK-4 cells and a one-step growth curve was determined. Detectable virion production was absent for approximately 20 h post-infection (p.i.) and was followed by a 4-day logarithmic phase of virus production. A …
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The development of penguinpox virus (PEPV) as a vaccine vector : transfer vector construction and rescue of virus growth in rabbit kidney cells (RK-13) by vaccinia virus K1
… the ability of a dual protein and Canarypox virus recombinant to protect humans against HIV-1 infections. Because poxviruses are being developed as vaccine vectors against a number of diseases, it is important to continue the search for novel poxvirus vectors, in particular, those that do not …
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Design of a Tetracycline Operon Inducible System for the Control of Vaccinia Virus Replication: Implications for Vaccine Development
<p>The use of vaccinia virus (VACV) as a vaccine resulted in the eradication of smallpox in 1979. Characteristics that contribute to the effectiveness of VACV as a vaccine and viral vector include its ability to elicit strong, long-lived humoral and cell-mediated immune responses as a …
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The interaction between NS1B protein of influenza B virus and the ubiquitin-like modifier ISG15 : insights into a unique species specific property of the virus
Influenza B virus causes a respiratory disease in people with a compromised immune system. The NS1B protein of influenza B virus is essential for virus growth and plays a crucial role in inhibiting the anti-viral responses mounted by the infected host cell. The N terminal 104 amino acids of NS1B …
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Construction of an <i>Escherichia coli lac</i> Repressor-Based System to Study Human Cytomegalovirus Gene Expression
… developed to study the roles of human cytomegalovirus (HCMV) genes during viral replication. To this end, a recombinant HCMV expressing the <em>lac</em> repressor was generated (RV<em>lac</em>), and an HCMV-specific promoter was targeted for conditional expression by inserting the <em>lac</em> …
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Generation and Characterization of Flavivirus Receptor-Binding Domain Mutants and Chimeras
Flaviviruses are positive-sense, single-stranded RNA viruses responsible for millions of infections annually. Primarily transmitted by ticks and mosquitoes, their associated diseases range from mild febrile infections to encephalitis and hemorrhagic fever. The molecular basis of the differences in …
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Reverse genetics system and fucntion of NSM protein of Rift Valley fever virus (family Bunyaviridae, genus phlebovirus)
Rift Valley fever virus (RVFV) (genus Phlebovirus, family Bunyaviridae) has a tripartite negative-strand genome, causes a mosquito-borne disease that is endemic in sub-Saharan African countries and results in large epidemics among humans and livestock. Furthermore, it has potential as a …
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The Diversion of Macromolecular Synthesis in L-Cells Towards Ends Dictated by Mengovirus
… biochemical and physiological studies on animal virus multiplication. Work of this kind with bacterial viruses had begun in the early 1950's and provided much information before research on animal viruses was initiated. The delay in the study of animal systems was due to the great difficulties …
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Comparative analysis of African and Asian/American Zika virus strains at the transcriptional and translational level
Zika virus (ZIKV) is an emerging mosquito-borne flavivirus. Initially, ZIKV was considered of low importance as infection was usually asymptomatic or caused mild illness. However, the emergence of the Asian/American lineage, in contrast to the African lineage, was considered a public health threat. …
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The role of the herpes simplex virus type 1 UL33 protein in DNA packaging
The UL33 gene of herpes simplex virus type 1 (HSV-1) encodes a 130 amino acid (aa) protein that is essential for the cleavage of concatemeric viral DNA into monomeric genomes and their packaging into preformed capsids. Several lines of evidence have suggested that UL33, along with the UL15 and UL28 …
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Transcriptome activity of human cytomegalovirus (strain Merlin) in fibroblasts, epithelial cells and astrocytes
Global human cytomegalovirus (HCMV) gene expression was investigated during replication in three permissive human cell types in tissue culture; human foetal foreskin fibroblasts (HFFF-2), human retinal pigmented epithelial (RPE) cells, and human astrocytoma cells (U373Mg). A custom HCMV DNA …
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Poliovirus Replication: Interactions with Cytoplasmic Membranes and with Compounds Which Affect Virus Biosynthesis
<p>Picornavirus multiplication is intimately associated with intracellular membranes and affects their production and distribution within the cell. We have shown that the density distribution of cytoplasmic membranes separated by isopycnic centrifugation in discontinuous sucrose gradients is …
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Relating the structure of the HSV-1 UL25 DNA packaging protein to its function
The herpes simplex virus type 1 (HSV-1) UL25 protein (pUL25) is a minor capsid protein that is essential for packaging the full-length viral genome into preformed precursor capsid. It is also important in virus entry and recently has been implicated in the egress of the virus from the cell (Coller …