Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Virus assembly"”.
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Statistical thermodynamics of virus assembly
… Three problems in understanding the capsid assembly for a retrovirus are studied: First, the way in which the viral membrane affects the structure of in vivo assembled HIV-1 capsid is studied. We show that conical and cylindrical capsids have similar energy at high surface tension of the …
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Optimized Microbial Recombinant Production of HIV-1 Anti-Envelope Antibody Fragments with Applications to Single Particle Tracking of Virus Assembly
… labeling and study of the human immunodeficiency virus type 1 (HIV-1) envelope (Env) protein during HIV viral assembly. There remains insufficient knowledge about the molecular dynamics and interactions of HIV-1 Env protein with its counterpart, Gag, on the inner host cell surface during assembly …
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Virus-enabled synthesis and 2D/3D assembly of nanowire networks
… tunable porosity. In this thesis, we explore the virus-enabled synthesis and two/three-dimensional assembly of metallic and semiconducting nanowire networks for future applications including batteries and solar cells. In Chapter 2, we describe the virus-enabled synthesis of titanium oxide …
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Migration of Nucleocapsids in Vesicular Stomatitis Virus-Infected Cells is Dependent on Microtubules and Actin Filaments
… of cytoskeletal motors and adapter proteins. Viruses hijack these pathways to distribute their proteins and genetic material. Vesicular stomatitis virus (VSV) is a prototype Rhabdovirus that has been used to study transport within the cell. We developed a new method of analysis of fluorescence …
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Relating the structure of the HSV-1 UL25 DNA packaging protein to its function
The herpes simplex virus type 1 (HSV-1) UL25 protein (pUL25) is a minor capsid protein that is essential for packaging the full-length viral genome into preformed precursor capsid. It is also important in virus entry and recently has been implicated in the egress of the virus from the cell (Coller …
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Site-Specific Labelling of the Flavivirus Envelope Protein using Genetic Code Expansion
Flaviviruses are arthropod-borne viruses that constitute a significant global public health burden. The envelope protein (E) orchestrates the processes of virus assembly, budding, and entry into cells and is the primary target of neutralising antibodies. The E proteins arranged on the surface of …
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Structural and functional studies of human ADAM 12 in myoblast fusion and Ebola virus VP40 in assembly
… II:<br></br>The Ebola virus matrix protein, VP40, is targeted to the plasma membrane where it is thought to induce assembly and budding of virions. Ebola virus VP40 is a monomer in solution consisting of two domains. Cellular localisation studies ofdifferent VP40 mutants …
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Investigating the Role of Stem-Loop 1 in the Assembly Process of HIV-1
… infectious HIV-1 particles is maturation of the virus core. This process is completed by cleavage of the capsid (CA) domain of Gag, from its precursor, CA-SP1, by the viral protease. Large deletions in stem-loop 1 (SL1) in the 5’ untranslated region (UTR) of HIV-1 genomic RNA (gRNA) delay CA-SP1 …
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Progress Towards Integrated Models of HCV Dynamics
<p>Hepatitis C virus is a small, enveloped positive-strand RNA member of the Flaviviridae and the etiological agent of a global epidemic of chronic hepatitis C. One of the salient features of HCV is a complex regulatory scheme involving numerous viral and cellular components and which may be the …
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Biogenesis, Assembly and Intracellular Trafficking of Junin Arenavirus Envelope Glycoprotein Complex
An unusual feature in the arenavirus envelope glycoprotein complex (GP-C) is the presence of a myristoylated stable signal peptide (SSP) in addition to the receptor binding subunit G1 and the transmembrane fusion subunit G2. Genetic studies were employed to understand the structure-function of the …
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Biogenesis, Assembly and Intracellular Trafficking of Junin Arenavirus Envelope Glycoprotein Complex
An unusual feature in the arenavirus envelope glycoprotein complex (GP-C) is the presence of a myristoylated stable signal peptide (SSP) in addition to the receptor binding subunit G1 and the transmembrane fusion subunit G2. Genetic studies were employed to understand the structure-function of the …
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Exploring the roles of key lipids in the influenza virus lifecycle
The influenza virus is a common respiratory pathogen with the capacity to cause global pandemics affecting millions of people, contributing substantially to overall mortality and morbidity. Thus, considerable research focuses on the prevention and treatment of influenza virus infection. Despite the …
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Foamy Virus Enzymes - Activity, Regulation and Resistance
Foamy viruses or spumaretroviruses belong to the family of retroviridae but differ in several aspects from other retroviruses (orthoretroviruses). Viral particles contain DNA not RNA. The Pol protein, the precursor of the viral enzymes, is translated from a separate mRNA independently of the capsid …
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Multi-modal imaging of herpesvirus infection
Herpes simplex virus (HSV)-1 is a large, enveloped DNA virus that undergoes a complex assembly pathway in the cell. Capsids assemble in the nucleus, cross the nuclear envelope into the cytoplasm by budding and fusion through the nuclear membranes, and acquire a proteinaceous ‘tegument’ layer in the …
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The contribution of viral and host cell factors to replication of the hepatitis C virus RNA genome
Studies on the hepatitis C virus (HCV) life cycle have been aided by the development of in vitro systems that permit replication of the viral RNA genome and virus particle production. However, the exact functions of the viral proteins, particularly those engaged in RNA synthesis, are poorly …
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Host specific gene expression of the baculovirus, Spodoptera frugiperda nuclear polyhedrosis virus, in insect cell lines
Baculoviruses (family: Baculoviridae) infect insects with high lethality and specificity and are experimental insecticides for suppressing pest populations in the field. Recently, recombinant DNA constructions of baculoviruses have been successfully utilized for the expression of foreign genes. In …
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Mechanisms of cell adhesion regulation by herpes simplex virus
Herpes simplex virus (HSV)-1 is a highly prevalent human pathogen that establishes a life-long infection. HSV-1 promotes its replication and spread by expressing multi-functional proteins that extensively remodel the host cell. Three such proteins are pUL21, pUL7 and pUL51. pUL21 is a viral …
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Statistical Mechanical Models Of Virus Capsid Assembly
Viruses have become an increasingly popular subject of physics investigation, particularly in the last decade. Advances in imaging of virus capsids-the protective protein shells-in a wide variety of stages of assembly have encouraged physical assembly models at a similarly wide variety of scales, …
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Synthesis and Microscopic Characterizations of Virus Nanoparticle-Based Composite Materials
<p>Plant viruses and virus-like protein cages (VLPs) have shown significant promise for a wide range of biomedical applications. In contrast to the artificial nanomaterials, VLPs exhibit unique properties including their well-organized three dimensional (3D) structures, monodispersity, …
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The Role Of The Membrane Proximal Region Of The M2 Cytoplasmic Tail In Virus Replication
Influenza A virus encodes M2, a proton channel that has been shown to be important during virus entry and assembly. The primary aim of this thesis was to investigate the role of the membrane proximal region, residues 46-69, of the M2 cytoplasmic tail during virus replication. A cholesterol …
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