Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 102 for “"Viral genome"”.
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Widespread Phosphorylation of H2AX by Adenovirus Requires Viral Genome Replication and the E1B-55k or E4orf3 Proteins
… DNA damage response and repair pathways. The viral E1B-55K and E4orf3 proteins prevent recognition of viral genomes as a substrate for DNA repair by targeting members of the sensor complex composed of Mre11/Rad50/NBS1 for degradation and relocalization, as well as targeting the effector …
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Characterization of an Amphipathic Alpha-Helix in the Membrane Targeting and Viral Genome Replication of Brome Mosaic Virus
… organelle membranes of host cells to establish viral replication complexes. The replication protein 1a of brome mosaic virus associates strongly with the nuclear endoplasmic reticulum (ER) membranes, invaginates membranes into the lumen, and recruits various host proteins to establish …
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Strucutre-function studies of the venezuelan equine encephalitis virus 5'UTR promoter element and its role in attenuation of the virus
… elements play critical roles in several viral processes. An \r\nattempt to elucidate the role of one such RNA structural element encoded by the 5’ \r\nUntranslated Region (5’UTR), in regulating virus replication and attenuation of \r\nVenezuelan equine encephalitis virus (VEEV) is …
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Structural and Functional Requirements for Homotypic and Heterotypic Interference by Indiana Serotype Defective Interfering Particles of Vesicular Stomatitis Virus
… interference. In the case of DI particles whose genomes mapped at the 5' end of the virion RNA, this target size corresponded to the entire DI particle RNA molecule regardless of whether it amounted to 10%, 30%, or 50% of the viral genome. These data were interpreted as demonstrating that both …
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Super-resolved volumetric optical imaging of virus-infected cells
… of SARS-CoV-2 accumulates at the membrane of the viral replication organelles, strongly suggesting that this is where the encapsidation of the viral genome by the nucleocapsid protein occurs. Imaging the assembly of the viral genome of an optimal and suboptimal LAIV virus in A549 cells highlighted …
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Construction of an <i>Escherichia coli lac</i> Repressor-Based System to Study Human Cytomegalovirus Gene Expression
… of human cytomegalovirus (HCMV) genes during viral replication. To this end, a recombinant HCMV expressing the <em>lac</em> repressor was generated (RV<em>lac</em>), and an HCMV-specific promoter was targeted for conditional expression by inserting the <em>lac</em> operator sequence. The …
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Mechanistic analysis of the Zika virus translation-replication switch
The genomes of positive-sense RNA viruses are required for both translation and replication during infection. These two processes are antagonistic in nature, each requiring the RNA template in opposite directions. Thus, the balance between these processes during infection must be tightly regulated. …
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FUNCTIONAL ANALYSIS OF THE UPSTREAM PSEUDOKNOT DOMAIN AND ITS SUBSTITUTION WITH A POLY(A) TRACT IN TOBACCO MOSAIC VIRUS (TMV)
… DOMAIN (UPD) OF TOBACCO MOSAIC VIRUS (TMV) GENOME PLAYS AN IMPORTANT ROLE DURING VIRUS REPLICATION AND TRANSLATION. THE FUNCTIONS OF UPD WERE ANALYZED USING A SUBSTITUTED MUTANT TMV-43A IN WHICH AN INTRODUCED 43 NT POLY(A) TRACT REPLACED THE UPD IN TMV GENOME. TMV-43A DISPLAYED REDUCED …
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The mechanism of component recognition of bipartite geminiviruses: Genetic studies of the replication of squash leaf curl virus, and, Genetic and functional studies of squash leaf curl virus coat protein AR1
… geminiviruses encode functions essential for viral infection: the A component replicates the viral genome and the B component systemically spreads the virus throughout the host. Thus, correct recognition between the two components is required to ensure a constant co-presence of the two viral …
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Modelling the Evolution of HIV-1 Protein-Coding Sequences with Particular focus on the early stages of Infection
… Thandi Wood, February 2010 The evolution of the viral genome sequence over the course of HIV-1 infection is of interest for vaccine and drug design, and for the development of effective treatment strategies. Characteristics of the transmitted viral genome that could render the virus more …
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Integrative analysis of transcriptional activity and genome architecture changes upon viral infections
… architecture and transcriptional activity during viral infections, I employed a genome-wide chromosome conformation capture approach (Hi-C) on infected murine and human cells and further enriched those libraries for genomic loci of interest and the viral genomes with biotinylated RNA baits. In …
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Decoding aberrant RNA Synthesis by the Influenza A Virus
… transcribing and replicating each segment of the viral genome. In addition to the full- length vRNAs and cRNAs, the FluPolA synthesizes aberrant RNA products, such as defective interfering RNAs (diRNAs) and mini viral RNAs (mvRNAs). The innate immune system serves as the body's primary defence …
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Phylogenetic Analysis of Human Cytomegalovirus pUS27 and pUS28: Ascertaining an Independent or Linked Evolutionary History
… co-evolution involves the acquisition of four virally encoded GPCR chemokine receptor homologs, products of the US27, US28, UL33 and UL78 genes. G protein-coupled receptors (GPCR) are the largest family of cell surface proteins, found in organisms from yeast to humans. In this research, …
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Characteristics of an Endonuclease Associated with Adenovirus Types 2 and 12
… cellular DNA which contains some integrated viral DNA. The species which cosediments with DNA extracted from the mature virions is homogeneously distributed. This species represents the intact viral genome. The species of DNA which sediments slowly is homogeneously distributed hybridizes only …
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Investigating the Role of Viral Long Non-Coding RNAs During Human Cytomegalovirus Latency
… RNAs that are amongst the most highly expressed viral genes during latency. Long non-coding RNAs are defined as RNAs exceeding 200 nucleotides in length that have little or no protein-coding potential. In this thesis, I have investigated the roles of these two long non-coding RNAs, RNA4.9 and …
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Quasivirus Infection Model Identifies a Novel Role for Protein Arginine Methyltransferase 1 in the HPV Lifecycle
… are non-enveloped viruses with histone wrapped genomes. To date over 700 PVs have been identified in a wide range of hosts including mammals, birds, reptiles, and fish. Over 400 HPVs have been identified and these viruses fall into 5 genera termed Alpha-papillomaviruses, Beta-papillomaviruses, …
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Structural Basis For Poliovirus Cloverleaf-Mediated Regulation Of Genome Replication
… short, positive-sense single-stranded RNA genomes. During the viral replication cycle, it is critical that the virus generates the appropriate amounts of viral proteins and genomic RNA. Upon entry in the cell, the viral genome is first used as a template for translation of viral genes and …
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Description of interactions of Ebola virus with a putative reservoir species using next-generation sequencing
… small RNAs, and the characterization of entire viral populations at incredible levels of detail. For this work, I exploited this technology to explore two aspects of the bat/virus nexus; namely the small RNA profile of infection, and the evolution of the virus in bat cells. The biology of the …
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Investigations into the viral molecular determinants of lethality of Punta Toro virus (Bunyaviridae, phlebovirus) in the Syrian hamster.
… induced illness, pathogenic mechanism and the viral ecology with respect to animal reservoirs and distribution. This dissertation addresses questions regarding viral genetics and their affect on virulence in the hamster model of Phlebovirus pathogenesis. This study reports that PTV strains …
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Understanding the determinants for substrate recognition, regulation of enzymatic activity and the development of broad-spectrum inhibitors of coronavirus 3-chymotrypsin-like proteases
… for the development of broad-spectrum anti-coronaviral therapeutics. Viral encoded 3-Chymotrypsin-like protease (3CLpro) is essential for viral polyprotein processing to release non-structural proteins that form the replicase complex machinery for viral genome replication. Due to its indispensable …
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