Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"Viral capsid"”.
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The Role of Linker 2 (L2) Region in RhTRIM5α Assembly and HIV-1 Restriction
… Several models have been proposed for retroviral restriction by TRIM5 proteins (TRIM5alpha and TRIMCyp). These models collectively suggest that TRIM5 proteins mediate restriction by recognizing specific determinants in the viral capsid and directly binding the capsid. Following binding, the …
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Immunochemical studies with tryptic peptides of tobacco mosaic virus protein
… and direct binding experiments. The viral subunit has been found to possess six antigenic determinants. Two of these, situated in tryptic peptide I (residues 1-41), were found to be a cryptotope (i.e. an antigenic determinant absent from the outer surface of the assembled viral …
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Medical Engineering and Medical Physics: Metagenomic Sequencing for Viral Diagnostics and Discovery
… with encephalitis with clinical suspicion of viral infection. These methods were used to interrogate secondary coinfections in patient cohorts with primary HIV and Lassa infection. Metagenomic sequencing can also be utilized to perform large scale screening for the directed evolution of viral …
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Intracellular sensor spatial multiplexing via RNA scaffolds
… Each sensor is fused to an orthogonal viral capsid protein that binds to a long, repetitive strand of its corresponding RNA sequence. All sensors fluoresce green and are indistinguishable during recording but are identified with post-hoc antibody or FISH staining for each …
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Host Proteins Interact with the Hiv-1 Core to Facilitate and Restrict
… termed restriction factors, which inhibit viral replication at various stages of the viral life cycle, determine the species-specific tropism of numerous retroviruses. Many members of the TRIM family of proteins act as viral restriction factors. One well-characterized example is the ability …
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Biotemplated resin and carbon nanomaterials for energy and environmental applications
… focused on genetically engineering the M13 viral capsid for interaction with inorganic materials, such as metals and oxides. In this thesis, the utility of the M13 toolkit was extended to the synthesis of organic and carbonaceous materials. Biotemplating of phenolic resins was extensively …
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Surface plasmon enhanced fluorescence for biological imaging : from visible to short-wave infrared
… nanoparticles and visible dye molecules on the viral capsid. Practical factors controlling fluorescence enhancement, such as nanoparticle size and dye-to-nanoparticle distance, are studied in this project. Lastly, the highly fluorescent probe is applied for in vitro staining of E.
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The Tradeoffs for a Viral Mutant with Enhanced Replication Speed
… speed. We found that a single mutation in a viral capsid protein, VP1-F106L, was sufficient for the fast-replication phenotype. Characterization of this mutant revealed quicker genome release during entry compared to wild-type virus, highlighting a previously unappreciated infection barrier. …
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UV light inactivation of adenovirus, coxsackievirus and murine norovirus
… genome of the virus, but also proteins in the viral capsid that may disturb the ability of the virus to attach to the host cell and therefore its ability to complete its infection cycle. The conditions of the water used for the experimental work were modeled after those encountered at the …
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New Biological Pathways
… For information retrieval, I propose repurposing viral capsid proteins to perform non-destructive transcriptomic measurements. We demonstrate that this approach allows for live-cell transcriptomics, and we longitudinally measure the transcriptional responses of the same living human cells after …
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Systems Engineering for Viral Vector Manufacturing
… of rAAVs. However, current state-of-the-art viral vector manufacturing methods still fall short of meeting current and future demands. Transient transfection via triple transfection of plasmids is the most common manufacturing platform for the clinical stage due to its flexibility in changing …
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Interfacial behavior of RNA-free MS2 bacteriophage particles
… material on the effective surface charge a viral capsid was investigated in this study. The RNA of bacteriophage MS2 was removed and the empty capsid characterized and compared to intact MS2. Transmission electron microscopy was used to ensure that the MS2 capsid was intact and RNA removed. …
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Molecular Identification of Plant Pathogens Affecting Cannabis sativa Crops in Nevada
… species based on rDNA sequences and analyzing viral capsid protein gene sequences, which enabled the successful identification of every encountered organism to a species level. The pathogens identified in this study include five Fusarium species: F. oxysporum, F. solani, F. equiseti, F. …
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Molecular Mechanisms of Protein Self-Assembly and Aggregation
… information on the microscopic mechanisms of viral capsid self-assembly from experimental data, synthesising the results from several different systems to draw general evolutionary conclusions about the assembly mechanism. Finally, we model the oligomerisation of Hsp70 thermodynamically and …
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Simple Physical Approaches to Complex Biological Systems
… the electrostatic potential at the surface of a viral capsid that is nearly half a million atoms in size. Next, a model of the nucleosome using simplified geometry is presented. This system is a complex of protein and DNA and acts as the first level of DNA compaction inside the nucleus of …
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Characterization of human astrovirus in pediatric patients with diarrhea from rural communities of Limpopo South Africa
… analysis of results were captured. The RIDA®GENE Viral Stool Panel I (PG1325) multiplex real-time RT-PCR assay was used for the detection of Astrovirus. Positive Astrovirus extracts were amplified by one-step ahead RT-PCR (cat no: 220213, (QIAGEN)) and one-step RT-PCR kit (cat no: 210212, …
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Novel intranasal GNRs-DNA vaccines against human papillomavirus infection
… HPV type 16 L1 gene for expression ofthe viral capsid-like particles in host cells. GNRs were functionalized with either PEIor PDDAC cationic polymers for high affinity to negatively charged DNAs. TheDNA is constructed in the backbone of a mammalian expression vector, plasmidpcDNA3 YFP of …
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Genetic and Biochemical Analyses of the Flaviviridae Capsid Proteins
… including hepatitis C, yellow fever, and bovine viral diarrhea. Efficient dissemination of these viruses is dependent on the production of infectious particles, thought to arise by budding of the capsid protein and associated genomic RNA through a host cell-derived lipid membrane outfitted with …
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Norovirus Translation and Replication
… currently there are no licensed vaccines or antivirals. The understanding of norovirus biology has been hampered by the inability to cultivate HuNoV in cell culture. To establish a tissue culture system, infectious HuNoVs were purified from clinical stool samples. HuNoV replication was tested in …
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