Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Viral assembly"”.
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C-terminus of Hepatitis B virus RNase H Domain is Important for HBV replication
… (RT), and RNase H, plays critical roles in viral assembly and replication. RNase H domain is required for HBV DNA replication, however critical motif or amino acid residues in the RNase H domain for the HBV replication has not been extrensively demonstrated yet. In the present study, several …
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Human coronavirus NL63 envelope protein-protein interactions: role in pathogenesis
… despite its critical functions in viral assembly and release, as well as its role as a major contributor to viral pathogenesis. Its contribution to viral pathogenesis has been strongly linked to a C-terminal PDZ-binding motif (PBM) that interacts with host post synaptic density …
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Single Molecule Imaging of Human Immunodeficiency Virus Type-1 (HIV-1) Envelope Glycoprotein Dynamics Identifies Viral Elements Contributing to Infectious Particle Assembly
… process in the life cycle of the virus: the assembly of new infectious virion, which propagate the infection within a host. Though it is known that the viral spike glycoprotein, Envelope (Env), responsible for viral entry into a host immune cell, is essential in propagation of infection, the …
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Early-stage drug discovery of HIV-1 frameshifting modulators
… Despite the success of highly active antiretroviral therapy (HAART), drug resistant viruses and adverse drug reactions raise the need for new classes of antiretroviral drugs. During HIV-1 replication, the viral frameshifting element orchestrates expression of a specific ratio of the essential …
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Optimized Microbial Recombinant Production of HIV-1 Anti-Envelope Antibody Fragments with Applications to Single Particle Tracking of Virus Assembly
… type 1 (HIV-1) envelope (Env) protein during HIV viral assembly. There remains insufficient knowledge about the molecular dynamics and interactions of HIV-1 Env protein with its counterpart, Gag, on the inner host cell surface during assembly of a mature virus particle. There also remains an …
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Structural and Biochemical Characterization of N-Terminal Protease of Classical Swine Fever Virus
… (Ser169). Npro is essential for initiation of viral polyprotein processing and generation of a viable core protein for viral assembly. The predicted catalytic triad of Npro is Glu22, His49 and Cys69, which differs from the known catalytic triads in either serine or cysteine proteases. Due to …
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Characterizing Roles of Host Cell Trafficking Factors in HIV Assembly and Release
… immunodeficiency syndrome (AIDS). Antiretroviral therapy (ART) can suppress HIV-1 replication, but there is still no vaccine or cure. Here, we have addressed open questions about two key steps of the HIV-1 life cycle which are not targeted by current ART and which rely crucially on host cell …
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A Study of the Membrane Binding Properties of the Matrix Protein from Human Respiratory Syncytial Virus
… structure of filamentous RSV virions with the viral envelope removed. These helical assemblies appeared to grow from liposomes, and demonstrate the inherent ability of RSV M to self assemble into arrays of this nature. Samples of purified protein were provided to Dr Victoria Money who was able …
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Studying viral-like particles using fluorescence fluctuation spectroscopy
… interactions is necessary to understand viral assembly, but it is notoriously difficult. Fluorescence fluctuation spectroscopy (FFS) is a promising experimental technique that determines the interactions of proteins and other biomolecules from fluorescence signal fluctuations with single …
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Causes and Consequences of Genetic Robustness and Fragility in HIV-1 Proteins
… However, the compact nature of the HIV-1 viral genome, in which small or overlapping proteins are often multifunctional and essential, often necessitates a strong pressure to conserve functional roles, which is at opposition with immunological pressures to diversify. Under such conditions, …
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Visualizing Protease Activation, Retrochmp3 Activity, and VPR Recruitment During HIV-1 Assembly
… covers my contributions to the field of HIV-1 assembly and the field of science communication. Over the course of my studies, I have determined when the HIV-1 protease becomes active during the assembly of new virions, elucidated the mechanism by which the protein retroCHMP3 has an antiviral …
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Relating the structure of the HSV-1 UL25 DNA packaging protein to its function
… that is essential for packaging the full-length viral genome into preformed precursor capsid. It is also important in virus entry and recently has been implicated in the egress of the virus from the cell (Coller et al., 2007, Preston et al., 2008). The crystallographic structure of an …
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COMPUTATIONAL MULTISCALE INVESTIGATIONS OF BIOLOGICAL MOLECULES
… insights into the dynamics and behavior of the viral components. The first set of simulations investigates the coarse-grained folding and relaxation of the RNA2 viral single-stranded RNA (ssRNA) fragment. The simulations start with a free, rod-like polymer chain configuration, and the subsequent …