Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 86 for “"Viral Vectors"”.
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Improving Viral Vectors for Gene Targeting in Gene Therapy
… have already been many clinical trials using viral vehicles for gene delivery. One problem with using a virus for gene therapy is the low titer associated with some types of virus, in particular, lentivirus. In the first part of this dissertation, this problem is addressed by showing that the …
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Developing Adeno-Associated Viral Vectors As A Gene Editing Platform
The success of adeno-associated viral (AAV) vectors for gene replacement applications has spurred interest to repurpose AAV into a platform for CRISPR/Cas9 gene editing. Yet, AAV vector behaviors that are critical for gene editing have been unexplored. To safely and effectively combine AAV and …
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Role of viral vectors in candidate HIV vaccine-induced immune responses
… in our knowledge of host responses to vaccine vectors in HIV vaccination. Using PBMC collected from participants in the RV144 and HVTN204 clinical trials who received an ALVAC- or Ad5-vectored vaccine, respectively, we show that ALVAC-specific CD4 T cells are significantly less susceptible to …
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Factors affecting transduction efficiency of pseudotyped viral vectors incorporating alphaviral glycoproteins
… used for delivering this gene are known as vectors. Retroviruses are popularly used gene therapy/transfer vectors. However, retroviruses are limited in the range of cells they can enter and infect. The range of cells targeted by the viral vector can be either expanded or narrowed by …
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On the Rational Design of Adeno-Associated Viral Vectors as Precision Tools in Neuroscience
Contains fulltext : 309684.pdf (Publisher’s version ) (Open Access)
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An investigation into the high level production of proteins in tobacco using transgenic plants or viral vectors
… inserted between the two UTRs. This artificial viral cDNA (5'cat3') was cloned immediately downstream of the cauliflower mosaic virus 35 S promoter at the transcription initiation site to make a DNA vector. An RNA vector construct was made by placing the 5'cat3' segment under the control of a T7 …
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Investigation of barriers to non-viral gene delivery and design of novel polymer-based gene delivery systems
… significant challenge facing gene therapy today. Viral vectors remain the dominant approach for addressing the delivery problem; however, concerns regarding the safety of viral vectors have resulted in an increasing interest in non-viral vectors. Non-viral vectors offer the promise of improved …
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Structure-Property-Transfection Relationships in Polycation-mediated Non-viral DNA Delivery
Non-viral gene delivery agents, such as cationic polyelectrolytes, are attractive replacements to viruses due to the absence of potential immunogenic risk and the ability to tune their macromolecular structure. Although non-viral vectors possess numerous design advantages, several investigators …
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Analysis of the role of lipids in retrovirus transduction
… in gene therapy protocols are mammalian virus vectors. Specifically, retroviruses are one of the most common viral vectors used since they are able to permanently integrate their transgene into the host cell genome, providing, in principal, to a long-term cure. The potential applications of …
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Quantitative analysis of non-viral gene therapy in primary liver culture systems
… novel combination therapies for cancers and viral infections, and offer a new and effective platform for next generation vaccines. However, after more than three decades of research and development efforts, clinical success has yet to be realized. Successful delivery of DNA is a crucial first …
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Hybrid Gene Therapy Vectors Comprised of Synthetic Polymer and Noninfectious Virus -Like Particles
While the hybrid vectors have combined some of the beneficial traits of the polymer and viral components the efficiency of the vector is low, in comparison to conventional viral vectors. Based on the changes in gene expression over time and the effect of chloroquine, the limiting step of hybrid …
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Making In Utero Gene Therapy Safer and More Efficient: A First Step Towards Clinical Realization
… an effort to improve the efficacy and safety of viral vectors. A two-vector delivery system was constructed to perform cell-specific in utero gene therapy, and I showed proof-of-principle that the system functions in vitro in human and sheep cells. An Ad5 serotype adenoviral vector (Ad5) acts as …
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The effect of nfa1 accination against Naeglearia fowleri infection
… of N. fowleri. In this study, the retroviral vector (pQCXIN) and the lentiviral vector (pCDH) cloned with the egfp-nfa1 gene was constructed and characterized for nfa1 DNA vaccination in mice. The expression of nfa1 gene in Chinese hamster ovary cell and human primary nasal epithelial …
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Cationic, α-helical polypeptides for cell penetration and non-viral gene delivery
… The development of effective and safe delivery vectors are quite crucial towards gene therapy. Compared with viral vectors, non-viral gene delivery vectors allow the safe delivery of genetic materials with less inherent adverse reactions such as immunogenicity, unexpected viral replication and …
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STUDYING THE INTERACTION OF NANO- & MICROPLASTICS WITH ENTEROVIRUS AND THEIR CYTOTOXICITY ON HUMAN LUNG CELL LINE
… charged surfaces, resulting in significant viral reduction. PMMA fibres containing 5 mg cetyltrimethylammonium bromide (CTAB) achieved the greatest reduction but retained infectivity, suggesting a possible role as viral vectors. Long-term exposure studies demonstrated rapid nanoplastic …
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Development of Extrachromosomal Genetic Technologies for Persistent and Tunable Expression of Therapeutic Payloads
… editing (e.g., CRISPR\Cas9) or integrating viral vectors (e.g., retrovirus, lentivirus). Integration-based approaches risk insertional mutagenesis and oncogene activation, yielding unpredictable, irreversible consequences. Inspired by latent DNA viruses with genomes persisting as episomes, …
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Axon/dendrite Targeting of Metabotropic Glutamate Receptors by Their Cytoplasmic Carboxy Terminal Domains
… targeted when expressed from defective viral vectors in cultured hippocampal neurons: group I mGluR1a and group II mGluR2 are targeted to dendrites and excluded from axons, whereas group III mGluR7 is targeted to axons and dendrites. Chimeras and deletions revealed that axon exclusion of …
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LIVER FIBROSIS IMPAIRS HEPATOCYTE TRANSDUCTION BY AAV VECTORS
Adeno-associated viral vectors (AAVs) are the most promising tools for liver directed gene therapy. However, integrity of hepatic architecture has been considered pre-requisite for efficient gene delivery and clinical studies have been addressed toward patients with no or negligible hepatic damage …
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Improving the delivery and efficacy of molecular medicine via extracellular matrix modulation : insights from intravital microscopy
… vector, we demonstrated that the spread of these viral vectors within tumors is limited by the fibrillar collagen in the extracellular matrix. Matrix modification via the introduction of bacterial collagenase along with the initial virus injection resulted in a significant improvement in the range …
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Engineering Viruses for Gene Therapy: Isolating and Characterizing Murine Leukemia Virus With Improved Stability
… widely used gene therapy vector. Such engineered viral vectors may prove useful for future gene therapies.
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