Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 55 for “"Viral Vector"”.
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Systems Engineering for Viral Vector Manufacturing
… of rAAVs. However, current state-of-the-art viral vector manufacturing methods still fall short of meeting current and future demands. Transient transfection via triple transfection of plasmids is the most common manufacturing platform for the clinical stage due to its flexibility in changing …
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Developing SINrep5 as a Viral Vector Platform to Test SARS-CoV-2 Vaccine Candidates
… and immunization in the arms race against novel viral agents. SARS-CoV-2 vaccines target the mutation-prone Spike Protein, leading to the rise of viral variants, and warranting additional research into the potential of diversifying vaccine target candidates, as well as vaccine options. One …
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Heterologous Prime-Boost strategies using live-attenuated and viral vector vaccines to combat pneumonic plague
… utilizing either 2-doses of an adenovirus vector (Ad5-YFV) or a heterologous strategy utilizing Ad5-YFV and a live-attenuated (LMA) vaccine were evaluated for protective efficacy against pneumonic plague. A single immunization event giving both Ad5-YFV and LMA at once was also tested as a …
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Heterologous Prime-Boost strategies using live-attenuated and viral vector vaccines to combat pneumonic plague
… utilizing either 2-doses of an adenovirus vector (Ad5-YFV) or a heterologous strategy utilizing Ad5-YFV and a live-attenuated (LMA) vaccine were evaluated for protective efficacy against pneumonic plague. A single immunization event giving both Ad5-YFV and LMA at once was also tested as a …
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Correction of AB Variant GM2 Gangliosidoses Using AAV9 Viral Vector Gene Therapy in a Mouse Model
… treatment of single stranded (ss) AAV9/GM2A viral vector therapy at a dose of 1 x 1014 vector genomes per kilogram of mouse and to see at least biochemical reduction in GM2 ganglioside storage in both the short- and long-term treated cohorts. ssAAV9/GM2A plasmid was created and tested in …
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Characterization and Development of a Novel Cytomegalovirus for Evaluation as an HIV Vaccine Viral Vector in a Non-Human Primate Model
… there is a real need to diversify the pool of vectors undergoing testing and to utilize vectors that can induce durable immunity. Herpesviruses, such as cytomegalovirus (CMV), show much promise as viral vectors, as they establish lifelong latency with periodic reactivation, enabling persistent …
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The effect of nfa1 accination against Naeglearia fowleri infection
… of N. fowleri. In this study, the retroviral vector (pQCXIN) and the lentiviral vector (pCDH) cloned with the egfp-nfa1 gene was constructed and characterized for nfa1 DNA vaccination in mice. The expression of nfa1 gene in Chinese hamster ovary cell and human primary nasal epithelial …
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Grapevine Fanleaf Virus: Biology, Biotechnology And Resistance
… on its satellite RNA, (2) engineer GFLV into a viral vector for grapevine functional genomics, and (3) discover a source of resistance to GFLV. This author addressed GFLV biology by studying the least understood aspect of GFLV: its satellite RNA. This author sequenced a new GFLV satellite RNA …
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Factors affecting transduction efficiency of pseudotyped viral vectors incorporating alphaviral glycoproteins
… used for delivering this gene are known as vectors. Retroviruses are popularly used gene therapy/transfer vectors. However, retroviruses are limited in the range of cells they can enter and infect. The range of cells targeted by the viral vector can be either expanded or narrowed by …
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Gene therapy for epilepsy: resculpturing synaptic transmission with neuropeptides
… has established a proof-of-principle for viral vector-mediated transfer of gene and expression of NPY in epileptogenic regions of the brain, providing effective suppression of both acute and chronic seizures in animal models of epilepsy. Therefore, NPY gene therapy strategies are currently …
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Design of a Tetracycline Operon Inducible System for the Control of Vaccinia Virus Replication: Implications for Vaccine Development
… to the effectiveness of VACV as a vaccine and viral vector include its ability to elicit strong, long-lived humoral and cell-mediated immune responses as a live-replicating virus and to accept large inserts of DNA into its genome. However, adverse events associated with its use as the smallpox …
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Quantitative analysis of adenoviral vector modification of a cytokine-mediated cell death decision
… viruses are used extensively as delivery vectors in clinical gene therapy and in molecular biology, but little is known about how the viral carrier itself contributes to cellular responses. In this thesis, we explored the link between viral vector modifications of signaling networks to …
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Studies of plasticity after neuronal injury
… of inhibitory extracellular matrix molecules. Viral vector technology was used to optimise the delivery of ChABC by gene transfer to host spinal cord cells. A lentiviral vector expressing the ChABC gene (LV-ChABC) was delivered to the adult rat spinal cord to achieve long-term delivery of …
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Targeted expression of the anti-HIV microbicide lectin griffithsin in maize and tobacco
… experimentation with different host systems, vector systems, codon optimisations and subcellular targeting. (Maclean et al., 2007; Yang et al., 2005). The latter sometimes results in unexpected locations which might reflect on an inherent property of the protein itself or specifically be …
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Generation of a FHV-1 Viral Vaccine Against Gonadotropin Releasing Hormone for Immunocontraception of Felines
… ovaries in felines, is the target of our vaccine vector. Expression of EGFP will allow tracking of the viral vector. The expression of the fusion protein (FliC-EGFP-GnRH) is expected to stimulate an antibody and cell mediated immune response directed towards feline GnRH, which will provide an …
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Cxcr2 Macromolecular Complex In Pancreatic Cancer: A Potential Therapeutic Target In Tumor Growth
… the PDZ motif) by adeno-associated virus type 2 viral vector potently suppresses human pancreatic tumor growth in immunodeficient mice. In summary, our results suggest the existence of a physical and functional coupling of CXCR2 and PLC-β3 mediated through NHERF1, forming a macromolecular complex …
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The toxicity and mutagenicity of the aflatoxin B₁ formamidopyrimidine DNA adduct
… adduct in a site specifically modified M13 viral vector transfected into E. coli. Four major results were concluded from this work. First, one of the rotamers of AFBI-FAPY is a strong block to DNA replication, even when bypass polymerases are employed by the cell.
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Mitochondrial response to axonal injury
… mitochondria in the axonal response to injury. A viral vector (AAV) containing a mitochondrially targeted fluorescent protein (mitoDsRed) as well as fluorescently tagged LC3 (GFP-LC3), an autophagosomal marker, was injected into primary motor cortex, to label the corticospinal tract (CST), of …
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Investigation of barriers to non-viral gene delivery and design of novel polymer-based gene delivery systems
… significant challenge facing gene therapy today. Viral vectors remain the dominant approach for addressing the delivery problem; however, concerns regarding the safety of viral vectors have resulted in an increasing interest in non-viral vectors. Non-viral vectors offer the promise of improved …
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